Systematic Postoperative Assessment of a Minimally-Invasive Sheep Model for the Treatment of Osteochondral Defects

Xin, Long; Mika, Joerg; Horbert, Victoria; Bischoff, Sabine; Schubert, Harald; Borowski, J; Maenz, Stefan; Huber, René; Sachse, A.; Illerhaus, Bernhard; Kinne, Raimund W.

doi:10.3390/life10120332

Cited by 1 publication

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“…In the transversal direction, maximal cartilage thickness was localized in the center of the MFC (points 5-7), suggesting that the central ridge of the MFC in its maximally accessible longitudinal extension during minimally invasive surgery may be the best location for intraoperatively created experimental cartilage defects. 14 …”

Section: Discussionmentioning

confidence: 99%

“…Thus, the present study aimed at a systematic comparison of the cartilage thickness on these stifle joint locations to identify the sites most suitable for experimental surgery with a minimally invasive, medial parapatellar approach. 14 In particular, the goal was to provide a detailed 3-dimensional mapping of the thickness at these implant sites, since previous studies had assessed specific selected spots in different large animals, however, without comprehensive mapping of the entire tibiofemoral joint surface. 12 , 15 - 22 …”

Section: Introductionmentioning

confidence: 99%

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Thickness of the Stifle Joint Articular Cartilage in Different Large Animal Models of Cartilage Repair and Regeneration

et al. 2020

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Objective Regulatory guidelines for preclinical cartilage repair studies suggest large animal models (e.g., sheep, goat, [mini]-pig, or horse) to obtain results representative for humans. However, information about the 3-dimensional thickness of articular cartilage at different implantation sites in these models is limited. Design To identify the most suitable site for experimental surgery, cartilage thickness at the medial femoral condyle (MFC), lateral femoral condyle (LFC), and trochlea in ovine, caprine, and porcine cadaver stifle joints was systematically measured using hematoxylin-eosin staining of 6 µm paraffin sections and software-based image analysis. Results Regarding all ventral-dorsal regions of the MFC, goat showed the thickest articular cartilage (maximal mean thickness: 1299 µm), followed by sheep (1096 µm) and mini-pig (604 µm), with the highest values in the most ventral and dorsal regions. Also for the LFC, the most ventral regions showed the thickest cartilage in goat (maximal mean thickness: 1118 µm), followed by sheep (678 µm) and mini-pig (607 µm). Except for the mini-pig, however, the cartilage thickness on the LFC was consistently lower than that on the MFC. The 3 species also differed along the transversal measuring points on the MFC and LFC. In contrast, there were no consistent differences for the regional cartilage thickness of the trochlea among goat and sheep (≥780 µm) and mini-pig (≤500 µm). Conclusions Based on their cartilage thickness, experimental defects on goat and sheep MFC may be viable options for preclinical cartilage repair studies, in addition to well-established horse models.

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Section: Discussionmentioning

confidence: 99%