Systematic purification of five glycosidases from Streptococcus (Diplococcus) pneumoniae

Glasgow, Lowrie R.; Paulson, James C.; Hill, Robert L.

doi:10.1016/s0021-9258(19)75265-x

Cited by 242 publications

(19 citation statements)

References 43 publications

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“…Cytosol used in transport assays was prepared from uninfected CHO wild-type cells as described previously (Beckers et al ., 1987;Beckers and Balch, 1989) . Endoglycosidase D (endo D) was obtained from Boehringer Mannheim Biochemicals (Indianapolis, IN) or prepared from the culture supernatant of Diplococcus pneumontae (Glasgow et al ., 1977) . Clones for rat rablb, and human rab 2, rab 3A, and rab 6 were obtained from A .…”

Section: Methodsmentioning

confidence: 99%

Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.

Plutner

Cox

Pind

et al. 1991

The Journal of Cell Biology

374

248

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Section: Methodsmentioning

confidence: 99%

Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.

Plutner

Cox

Pind

et al. 1991

The Journal of Cell Biology

374

248

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“…NaB2H4 and sialidase from Arthrobacter ureafaciens were obtained from Nacalai Tesque Co., Kyoto. Diplococcal /3-galactosidase and jd-A-acetylhexosaminidase were purified from culture fluid of Diplococcus pneumoniae according to the method of Glasgow et al (1977). Jack bean jd-A-acetylhexosaminidase and a-mannosidase were purified from jack bean meal (Sigma Chemical Co., St. Louis, MO)…”

Section: Methodsmentioning

confidence: 99%

Structural study of the sugar chains of human leukocyte common antigen CD45

Satô¹,

Furukawa²,

Autero³

et al. 1993

Biochemistry

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The leukocyte cell surface glycoprotein CD45 is a protein tyrosine phosphatase and is involved in signal transduction mediated by the T cell antigen receptor. The asparagine-linked sugar chains were released as oligosaccharides from purified CD45 by hydrazinolysis. Approximately 6 mol of sugar chains was released from 1 mol of CD45. These sugar chains were converted to radioactive oligosaccharides by reduction with NaB3H4 and separated into neutral and acidic fractions by paper electrophoresis. All of the acidic oligosaccharides were converted to neutral ones by digestion with sialidase, indicating that they are sialyl derivatives. Binding of the sialylated oligosaccharides to an SNA-agarose column as well as methylation analysis revealed that the oligosaccharides have only alpha-2,6-linked sialic acid residues. The neutral and sialidase-treated acidic oligosaccharides were fractionated by serial lectin column chromatography followed by Bio-Gel P-4 column chromatography. Structural studies of each oligosaccharide by sequential exo- and endoglycosidase digestion and by methylation analysis revealed that CD45 contains mainly bi-, tri-, and tetraantennary complex-type sugar chains. About 46% of the tetraantennary complex-type sugar chains had the poly(N-acetyllactosamine) groups and 18% of the 2,4-branched triantennary complex-type sugar chains had the fucosyl N-acetyllactosamine group. A portion of the bi- and 2,4-branched triantennary complex-type sugar chains were bisected. In addition to these sugar chains, a small amount of high mannose-type and hybrid-type sugar chains were detected.

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“…Trans asS-label ([35S]methionine and [35S]cysteine, >1,000 Ci/mmol) was purchased from ICN Biomedicals, Inc. (Irvine, CA). Endoglycosidase D (Endo D) t was either purchased from Boehringer-Mannheim Biochemicals (Indianapolis, IN), or prepared from the culture supernatant of Diplococcus pneumoniae (Glasgow et al, 1977). Nucleotide analogues were purchased in the form of their lithium salts from Boehringer-Mannbeim Biochemicals.…”

Section: Methodsmentioning

confidence: 99%

Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus.

Beckers

Balch

1989

The Journal of Cell Biology

345

162

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Abstract. Ca 2÷ and GTP hydrolysis are shown to be required for the transport of protein between the ER and the cis-Golgi compartment in semiintact cells, an in vitro system that reconstitutes transport between intact organelles. Transport was inhibited rapidly and irreversibly in the presence of micromolar concentrations of the nonhydrolyzable GTP analogue, GTP3,S. The transport block in the presence of GTP3,S was found to be distal to a post-ER, pre-Golgi compartment where proteins accumulate during incubation at 15°C. In addition, transport was completely inhibited in the absence of free Ca 2÷. A sharp peak defining optimal transport between the ER and the cis-Golgi was found to occur in the presence of 0.1 #M free Ca 2÷. Inhibition of transport in the absence of free Ca 2÷ was found to be fully reversible allowing the step inhibited by GTP'rS to be assigned to a position intermediate between the ER and the Ca 2÷ requiring step. The results suggest that GTP hydrolysis may trigger a switch to insure vectorial transport of protein along the ER/Golgi pathway, and that a free Ca 2÷ level similar to the physiological levels found in interphase cells is essential for a terminal step in vesicle delivery to the cis-Golgi compartment.

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Systematic purification of five glycosidases from Streptococcus (Diplococcus) pneumoniae

Cited by 242 publications

References 43 publications

Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.

Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.

Structural study of the sugar chains of human leukocyte common antigen CD45

Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus.

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