Systematic revelation of the protective effect and mechanism of Cordycep sinensis on diethylnitrosamine-induced rat hepatocellular carcinoma with proteomics

Abstract

Cordyceps sinensis (C. sinensis) has been reported to treat liver diseases. Here, we investigated the inhibitory effect of C. sinensis on hepatocarcinoma in a diethylnitrosamine (DEN)-induced rat model with functional proteome tools.In the DEN-exposed group, levels of serum alanine aminotransferase and aspartate aminotransferase were increased while C. sinensis application remarkably inhibited the activities of these enzymes. Histopathological analysis also indicated that C. sinensis could substantially restore hypertrophic hepatocytes caused by DEN, suggesting that C. sinensis is effective in preventing DEN-induced hepatocarcinogenesis.We therefore comprehensively delineated the global protein alterations using a proteome platform. The most meaningful changes were found among proteins involved in oxidative stress and detoxification. Meanwhile, C. sinensis application could attenuate the carbonylation level of several enzymes as well as chaperone proteins. Network analysis implied that C. sinensis could obviously alleviate hepatocarcinoma via modulating redox imbalance, protein ubiquitination and tumor growth–associated transcription factors.Our findings provide new insight into the potential effects of C. sinensis in preventing carcinogenesis and might help in developing novel therapeutic strategies against chemical-induced hepatocarcinoma.

Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid

“…HCC encompasses a spectrum of hepatic pathology. Similar to previous study 31 , levels of serum ALT and AST were increased in DEN group. CaA or ChA treatment remarkably inhibit the activities of these enzymes, and these effects could be confirmed by histopathological observation.…”

Red Raspberry Extract Protects the Skin against UVB-Induced Damage with Antioxidative and Anti-inflammatory Properties

“…A high-performance liquid chromatographic (Shimadzu SCL-10A VP) coupled with an SPD-M10A VP diode array detector was performed for the qualitative determination of compounds in the RBE [24].…”

Proteomic Research on the Antitumor Properties of Medicinal Mushrooms

“…This included a significant changes in expression of several ROS-related proteins, including catalase, DHE3 (glutamate dehydrogenase 1), PRDX1 (peroxiredoxin-1), GSTP (glutathione S-transferase P), GSTM1, and GSTM2 (glutathione S-transferase Mu 1 and 2). From these results, it is clear that C. sinensis eliminates ROS, resulting in a significant reduction of the level of redox state-correlated proteins [137]. 2-DE oxyblot analysis demonstrated that C. sinensis could reverse protein carbonylation induced by DEN.…”

“…Wang et al [137] analyzed the effects of Cordyceps sinensis treatment on the liver proteome of rats in which the hepatocellular carcinoma was induced by diethylnitrosamine (DEN). Cordyceps sinensis powder was used to prepare an extract that was analyzed with HPLC.…”