Systematic review of islet cryopreservation

Kojayan, Greg Garo; Alexander, Michael P.; Imagawa, David K.; Lakey, Jonathan R. T.

doi:10.1080/19382014.2017.1405202

Cited by 33 publications

(24 citation statements)

References 59 publications

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“…In addition, cell-based therapy does not prevent the possibility of whole-organ transplantation later. However, using mature cells for this therapy is limited due to the inability of these cells to proliferate in vitro, due to the metabolic damages induced by cryopreservation and to organ shortage as well [27,28,29].…”

Section: Cell-based Therapymentioning

confidence: 99%

Thrombogenic Risk Induced by Intravascular Mesenchymal Stem Cell Therapy: Current Status and Future Perspectives

Coppin

Sokal

Stéphenne

2019

Cells

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Mesenchymal stem cells (MSCs) are currently studied and used in numerous clinical trials. Nevertheless, some concerns have been raised regarding the safety of these infusions and the thrombogenic risk they induce. MSCs express procoagulant activity (PCA) linked to the expression of tissue factor (TF) that, when in contact with blood, initiates coagulation. Some even describe a dual activation of both the coagulation and the complement pathway, called Instant Blood-Mediated Inflammatory Reaction (IBMIR), explaining the disappointing results and low engraftment rates in clinical trials. However, nowadays, different approaches to modulate the PCA of MSCs and thus control the thrombogenic risk after cell infusion are being studied. This review summarizes both in vitro and in vivo studies on the PCA of MSC of various origins. It further emphasizes the crucial role of TF linked to the PCA of MSCs. Furthermore, optimization of MSC therapy protocols using different methods to control the PCA of MSCs are described.

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Section: Cell-based Therapymentioning

confidence: 99%

Thrombogenic Risk Induced by Intravascular Mesenchymal Stem Cell Therapy: Current Status and Future Perspectives

Coppin

Sokal

Stéphenne

2019

Cells

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“…Since β cells are easier available and capable of secreting insulin, their encapsulation in hydrogel could thus mimic real islet to regulate blood glucose. Different from the traditional islet cryopreservation methods including a high concentration of permeable cryoprotectants (pCPAs, like dimethyl sulfoxide, glycerol, ethylene glycol and propylene glycol) to prevent ice crystal damages, recent preservation methods enable efficient storage of various cells with hydrogel encapsulation, electromagnetic fields, nanoparticles, etc . Researches have been carried out and showed that by using droplet microfluidics numerous functional materials based on hydrogels could be fabricated .…”

Section: Introductionmentioning

confidence: 99%

Cold‐Responsive Nanocapsules Enable the Sole‐Cryoprotectant‐Trehalose Cryopreservation of β Cell–Laden Hydrogels for Diabetes Treatment

Cheng

Zhang

et al. 2019

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Islet transplantation has been one promising strategy in diabetes treatment, which can maintain patient's insulin level long‐term and avoid periodical insulin injections. However, donor shortage and temporal mismatch between donors and recipients has limited its widespread use. Therefore, searching for islet substitutes and developing efficient cryopreservation technology (providing potential islet bank for transplantation on demand) is in great need. Herein, a novel cryopreservation method is developed for islet β cells by combining microfluidic encapsulation and cold‐responsive nanocapsules (CR‐NCs). The cryopreserved cell‐laden hydrogels (calcium alginate hydrogel, CAH) can be transplanted for diabetes treatment. During the freezing process, trehalose is released inside β cells through the CR‐NCs and serves as the sole cryoprotectant (CPA). Additionally, CAH helps cells to survive the freeze–thaw process and provide cells with a natural immune barrier in vivo. Different from traditional cryopreservation methods, this method combining the CR‐NCs and hydrogel encapsulation replaces the toxic CPAs with natural trehalose. Great preservation results are obtained and transplantation experiments of diabetic rats further prove the excellent glucose regulation ability of such β cell–laden hydrogels post cryopreservation. This novel cryopreservation method helps to establish a reliable and ready‐to‐use bank of biological samples for transplantation therapy and other biomedical applications.

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“…The cryopreservation of stem cells and embryos has been successful (10,11). The cryopreservation of rat testicular tissue (12), rat, pig and human islet tissue (13)(14)(15)(16) and liver tissues of mice, rats, dogs, monkeys and humans has also been successful (17). Cryopreservation is not only conducive to the accumulation of donor tissue, but also significantly reduces its immunity (18).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effects of three cryoprotectants on the cryopreservation of mouse subcutaneous tissue under different conditions

et al. 2020

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The subcutaneous tissue of animals contains different cell types, and different cells have different requirements for cryopreservation. This establishes obstacles that need to be overcome in the clinical application of tissue preservation. In the present study, the effects of different freezing rates and various concentrations of cryoprotectants on the cryopreservation of subcutaneous tissue of mice were compared, and these results provided basic research data that can be used to explore the optimal cryopreservation method for tissue. The effects of three cryoprotectants, dimethyl sulfoxide, glycerinum and 1,2-propanediol, and their concentrations on the cryopreservation of subcutaneous tissue of mice were compared with slow and rapid freezing rates. The results revealed that under various cryopreservation conditions, the percentage of fibroblasts that grow from the tissue following slow cryopreservation (19.8%) was significantly higher than that following rapid freezing (6.7%) at osmotic equilibrium for 10-20 min (P<0.05). After 19 days of culture, under the conditions of slow freezing, with 10, 20 and 30% glycerinum as a cryoprotectant, respectively, fibroblasts grew from 26.0, 16.7 and 16.7% of the tissues, respectively. No fibroblasts were indicated in the tissue mass cultured in any other tissue blocks treated with cryopreservation solutions. Under the condition of rapid freezing, fibroblasts grew from 6.7 and 6.7% tissue blocks of 20% DMSO and 10% glycerinum, respectively, following 19 days of culture. No fibroblasts were identified in the tissue mass cultured in the other tissue blocks treated with cryopreservation solutions, and no fibroblasts were identified in the tissue blocks without osmotic balance before freezing.

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Systematic review of islet cryopreservation

Cited by 33 publications

References 59 publications

Thrombogenic Risk Induced by Intravascular Mesenchymal Stem Cell Therapy: Current Status and Future Perspectives

Thrombogenic Risk Induced by Intravascular Mesenchymal Stem Cell Therapy: Current Status and Future Perspectives

Cold‐Responsive Nanocapsules Enable the Sole‐Cryoprotectant‐Trehalose Cryopreservation of β Cell–Laden Hydrogels for Diabetes Treatment

Comparison of the effects of three cryoprotectants on the cryopreservation of mouse subcutaneous tissue under different conditions

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