Systematic review of liposomes’ characterization methods

Kanásová, Mária; Nesměrák, Karel

doi:10.1007/s00706-017-1994-9

Cited by 47 publications

(25 citation statements)

References 156 publications

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“…The extent of current reduction and the frequency of the pulses are related to the particle size and concentration of the nanocarrier sample, respectively. Particles are driven either by electrophoresis and electroosmosis or by pressure generated from a pressure module [ 100 , 101 ]. SIOS is a useful method to analyse multiple parameters of nanocarriers on a particle-by-particle basis.…”

Section: Methods Of Analysismentioning

confidence: 99%

“…For instance, it is problematic to choose a suitable elastic pore for polydisperse samples to avoid detecting several particles at the same time. Moreover, it is still difficult to detect only one particle at the time, and the data acquired with different nanopore sizes cannot be compared in parallel [ 101 ].…”

Section: Methods Of Analysismentioning

confidence: 99%

“…FCM is a very quick, reliable, robust and reproducible method. However, when employing light scattering detection, its operation can be disturbed by noisy signals from buffers, optics or electronics [ 98 , 101 , 102 ].…”

Section: Methods Of Analysismentioning

confidence: 99%

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Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems

et al. 2018

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Lipid-based drug delivery systems, or lipidic carriers, are being extensively employed to enhance the bioavailability of poorly-soluble drugs. They have the ability to incorporate both lipophilic and hydrophilic molecules and protecting them against degradation in vitro and in vivo. There is a number of physical attributes of lipid-based nanocarriers that determine their safety, stability, efficacy, as well as their in vitro and in vivo behaviour. These include average particle size/diameter and the polydispersity index (PDI), which is an indication of their quality with respect to the size distribution. The suitability of nanocarrier formulations for a particular route of drug administration depends on their average diameter, PDI and size stability, among other parameters. Controlling and validating these parameters are of key importance for the effective clinical applications of nanocarrier formulations. This review highlights the significance of size and PDI in the successful design, formulation and development of nanosystems for pharmaceutical, nutraceutical and other applications. Liposomes, nanoliposomes, vesicular phospholipid gels, solid lipid nanoparticles, transfersomes and tocosomes are presented as frequently-used lipidic drug carriers. The advantages and limitations of a range of available analytical techniques used to characterize lipidic nanocarrier formulations are also covered.

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Section: Methods Of Analysismentioning

confidence: 99%

Section: Methods Of Analysismentioning

confidence: 99%

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Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems

et al. 2018

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“…DLS also provides information on zeta potential by assessing changes in the scattered light intensity caused by particle motion due to the applied electric field. The access to the microscopic observation (TEM and even better cryo-TEM) is important as it provides the direct visualization of the individual particles with an accurate determination of size, shape, and morphology [ 24 ]. For in vivo applications, much work has been devoted to cover the liposomal surface with long polyethylene glycol (PEG)-containing chains, with the aim of masking the charges that are responsible for the recognition (and elimination from circulation) from the reticulo-endothelial system (RES) [ 25 ].…”

Section: Generalities About Liposomesmentioning

confidence: 99%

Liposome-Based Bioassays

Sforzi

Palagi

Aime

2020

Biology

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This review highlights the potential of using liposomes in bioassays. Liposomes consist of nano- or micro-sized, synthetically constructed phospholipid vesicles. Liposomes can be loaded with a number of reporting molecules that allow a dramatic amplification of the detection threshold in bioassays. Liposome-based sensors bind or react with the biological components of targets through the introduction of properly tailored vectors anchored on their external surface. The use of liposome-based formulations allows the set-up of bioassays that are rapid, sensitive, and often suitable for in-field applications. Selected applications in the field of immunoassays, as well as recognition/assessment of corona proteins, nucleic acids, exosomes, bacteria, and viruses are surveyed. The role of magnetoliposomes is also highlighted as an additional tool in the armory of liposome-based systems for bioassays.

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“…Lipids with varying chain lengths and different degrees of saturation can be introduced to tune them for different applications. 1–5 They are readily prepared using techniques like extrusion, sonication and rapid ethanol injection. Each of these methods influences properties such as size (diameter), lamellarity (single bilayer or multi-bilayer), and polydispersity (range of sizes).…”

Section: Introductionmentioning

confidence: 99%

Investigation of DPPC liposomes reveals their capability to entrap Aroclor 1260, an emerging environmental pollutant

Lozano

Rieth

2019

Preprint

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32Persistent organic pollutants (POPs) are a class of organic compounds that can accumulate in biological 33 and ecological environments due to their resistive nature to chemical, thermal and photo degradation. 34Polychlorinated biphenyls (PCBs) are a class of man-made POPs that saw wide-spread use in commercial 35 and industrial infrastructure as both an insulator and coolant in electrical transformers and capacitors. 36 2,2',3,3',4,4'-hexachlorobiphenyl (HCBP) was one of the most widely produced PCBs. As these 37 mechanical structures fail or are decommissioned, PCBs are released into the soil, migrate to the water 38 table, and eventually spread to nearby ecosystems by rain and wind. The stability of POPs and specifically 39 PCBs leave few options for environmental waste removal. Conventionally, liposomes have been used for 40 their drug delivery capabilities, but here we have chosen to investigate their capability in removing this 41 class of emerging environmental pollutants. Liposomes are small, nonpolar lipid bi-layered aggregates 42 capable of capturing a wide variety of both polar and nonpolar compounds. 43 Dipalmitoylphosphatidylcholine (DPPC) is a well-characterized lipid that can be derived from natural 44 sources. It is a phospholipid typically found as a major component of pulmonary surfactant mixtures. 45Liposomes were prepared using probe-tip sonication for both direct and passive incorporation of the HCBP 46 compound. Assimilation was assessed using both differential scanning calorimetry and UV-Vis 47 spectroscopy. After direct incorporation of HCBP the phase transition temperature, Tm, decreased from 48 40.8 °C to 37.4 °C. A subsequent UV-Vis analysis of HCBP by both direct and passive incorporation 49 showed an increase in HCBP incorporation proportionate to the length of exposure time up to 24 hours and 50 relative to the initial quantity present during the direct incorporation. Together the decrease in Tm and 51 increase in absorbance are indicative of HCBP incorporation and further demonstrate the potential for their 52 use as a method of sustainable environmental cleanup. 53 54 55 56 57 according to the calculated ratios of HCBP:DPPC and summarized in Table 1. The dried lipid films were 131 stored long-term at -20 °C. 132

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Systematic review of liposomes’ characterization methods

Cited by 47 publications

References 156 publications

Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems

Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems

Liposome-Based Bioassays

Investigation of DPPC liposomes reveals their capability to entrap Aroclor 1260, an emerging environmental pollutant

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