Systematic screening and structural characterization of dipeptides using offline 2D LC-LTQ-Orbitrap MS: A case study of Cordyceps sinensis

Li, J.; Yao, Changliang; Li, Yun; Qian, Zhengming; Wei, Wenlong; Li, Jiayuan; Bi, Qirui; Li, Wenjia; Cui, Yaokui; Guo, Dan

doi:10.1016/j.jpha.2021.07.007

Cited by 12 publications

(4 citation statements)

References 24 publications

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“…Therefore, the number of peptides detected by the Q-Exactive instrument is more than that detected by LTQ-Orbitrap. However, the peptides identified by LTQ-Orbitrap MS are generally longer than those identified by the Q-Exactive instrument [13,14]. In addition, the BLAST comparison revealed that target peptides were speciesspecific for the Bufo bufo gargarizans.…”

Section: Discussionmentioning

confidence: 98%

“…Species identification methods based on peptides have been established by researchers and applied in traditional Chinese medicine, such as deerskin [20], oil [21], and Dilaudid [22]. Considering the results of peptide identifications in the cinobufacini tablet and capsule, we determined four peptides as target peptides, including AVFPSIVGRPR (Pep1), PEPAKSAPAPK (Pep2), GIADALGKAYH (Pep3), and GVTIAQGGVLPNIQ (Pep4), which with high reproducibility and responsiveness [13,14]. The Q-Exactive MS showed high efficiency and quality [23], and was used to identify the peptides of cinobufacini injections in our research.…”

Section: Discussionmentioning

confidence: 99%

“…In the previous study, we used mass spectrometry to identify and analyze the peptides in the toad skin, cinobufacini capsule, and tablet. By combining qualitative analysis, reaction intensity, occurrence frequency, precursor protein function, and Basic Local Alignment Search Tool (BLAST) homology comparison results, four peptides with high reproducibility, response intensity, and species specificity were selected [13,14]. In this study, we will verify the existence of four peptides in cinobufacini injection, and establish a quantitative method by UHPLC-MS/MS to evaluate the content of target peptides in different batches of cinobufacini injections and toad skins from different localities.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of peptide components in cinobufacini injection and toad skin by ultra‐high‐performance liquid chromatography/triple quadrupole mass spectrometry

Zhang

Dong

et al. 2023

J of Separation Science

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Cinobufacini injection is commonly used in the clinical treatment of tumors and hepatitis B, but the quality is uneven. Currently, the main focus of its quality assessment is on steroids and alkaloids. Based on a previous study, we screened four peptides with high reproducibility, responsiveness, and specificity. This research was the first to develop an ultra‐high‐performance liquid chromatography/triple quadrupole mass spectrometry approach for evaluating the quality of cinobufacini preparations from the peptide perspective. In this study, we have identified 230 peptides in cinobufacini injection by Q‐Exactive mass spectrometry, which contains species‐specific peptides. Then, we used ultra‐high‐performance liquid chromatography/triple quadrupole mass spectrometry to establish a quantitative method for species‐specific peptides and carried out method validation. The result revealed that four peptides were linear in a specific range, and had great reproducibility, accuracy, and stability. Eventually, we evaluated the quality of eight batches of cinobufacini injections and 26 batches of toad skins using the total content of target peptides as the criterion. The outcomes demonstrated that the quality of cinobufacini injection is generally stable and the toad skin from Shandong is of the best quality. In conclusion, the quantitative approach that focuses on peptides will offer innovative perspectives on assessing the quality of cinobufacini preparations.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantification of peptide components in cinobufacini injection and toad skin by ultra‐high‐performance liquid chromatography/triple quadrupole mass spectrometry

Zhang

Dong

et al. 2023

J of Separation Science

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“…Yao et al [14] isolated and quantified the major P. notoginseng saponins such as Rg1 and Re, which are difficult to isolate, by 2D-LC. In current macromolecule assays, 2D-LC is mainly applied to proteins [18][19][20], and less of the literature deals with analyzing saccharide substances [21,22].…”

Section: Introductionmentioning

confidence: 99%

An Analysis of Polysaccharides from Eight Plants by a Novel Heart-Cutting Two-Dimensional Liquid Chromatography Method

Wang,

Jin,

Chai

et al. 2024

Foods

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Natural polysaccharides are important active biomolecules. However, the analysis and structural characterization of polysaccharides are challenging tasks that often require multiple techniques and maps to reflect their structural features. This study aimed to propose a new heart-cutting two-dimensional liquid chromatography (2D–LC) method for separating and analyzing polysaccharides to explore the multidimensional information of polysaccharide structure in a single map. That is, the first-dimension liquid chromatography (1D–LC) presents molecular-weight information, and the second-dimension liquid chromatography (2D–LC) shows the fingerprints of polysaccharides. In this 2D–LC system, the size-exclusion chromatography–hydrophilic interaction chromatography (SEC–HILIC) model was established. Coupling with a charged aerosol detector (CAD) eliminated the need for the derivatization of the polysaccharide sample, allowing the whole process to be completed within 80 min. The methods were all validated in terms of precision, linearity, stability, and repeatability. The capability of the new 2D-LC method was demonstrated in determining various species of natural polysaccharides. Our experimental data demonstrated the feasibility of the whole systematic approach, opening the door for further applications in the field of natural polysaccharide analysis.

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Green Chromatography Techniques

Goyal

Sharma

Singh³

et al. 2022

Green Chemical Analysis and Sample Preparations

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Systematic screening and structural characterization of dipeptides using offline 2D LC-LTQ-Orbitrap MS: A case study of Cordyceps sinensis

Cited by 12 publications

References 24 publications

Quantification of peptide components in cinobufacini injection and toad skin by ultra‐high‐performance liquid chromatography/triple quadrupole mass spectrometry

Quantification of peptide components in cinobufacini injection and toad skin by ultra‐high‐performance liquid chromatography/triple quadrupole mass spectrometry

An Analysis of Polysaccharides from Eight Plants by a Novel Heart-Cutting Two-Dimensional Liquid Chromatography Method

Green Chromatography Techniques

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