Systematic truncating of aptamers to create high-performance graphene oxide (GO)-based aptasensors for the multiplex detection of mycotoxins

Abstract

An aptamer-truncating strategy was utilized to create high-performance GO-based aptasensors for detection of multiple mycotoxins.

Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt

“… Detection system LOD Sample Ref. A fluorescence aptasensor based on semiconductor quantum dots and MoS 2 nanosheets 1 ng/mL Red wine ( Lu et al, 2017 ) A dual-channel fluorescence aptasensor based on Texas red-labelled P-OTA-36 and GO 15.17 ng/mL White wine ( Wang et al, 2019 ) Aptamer-based fluorescent sensor by quenching of gold nanoparticles 9.148 ng/mL Cornmeal, beer, coffee ( Lv et al, 2017 ) An aptasensor based on single-walled carbon nanohorn 6.932 ng/mL Red wine ( Lv et al, 2016 ) A fluorescence aptasensor based on a double-stranded DNA competition mode and GO nanosheets 0.011 ng/mL Malt This work …”

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

“…In this case, a conformational change induced by binding of the target to the aptamer results in an alteration of the fluorescent signal. Both fluorescently-labeled and label-free biosensing approaches have been reported with the possibility of on-site detection using portable analyzers [ 56 , 57 , 58 , 59 ]. The target of interest can range from small molecules such as the mycotoxins aflatoxin B1 [ 60 ] and ochratoxin A [ 61 ], bisphenol A [ 62 ], the antibiotic tobramycin [ 54 ] and the marine toxin okadaic acid [ 63 ], to larger entities such as lipopolysaccharides [ 64 ] and the cyclic heptapeptide microcystin-LR (a hepatotoxin produced by cyanobacteria) [ 65 ].…”

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

“…At present, fluorescent biosensors are the most popular optical sensors because of their high sensitivity, stable signal, and fast response. Various nano-materials such as magnetic nanoparticles (MNPs), quantum dots (QDs), carbon nanoparticles (CNPs), gold nanoparticles (AuNPs), fluorescent nanobeads, and graphene oxide (GO) have been widely applied in construction of fluorescent sensors [78][79][80]. Lanthanide-based luminescent materials such as upconverting nanoparticles (UCNPs) and time-resolved fluorescence materials (TRFMs) have also become popular in recent years [81,82].…”