Systematics and phylogeography of the Dactylorhiza maculata complex (Orchidaceae) in Scandinavia: insights from cytological, morphological and molecular data

Ståhlberg, David; Hedrén, Mikael

doi:10.1007/s00606-008-0035-x

Cited by 33 publications

(43 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An origin during the Holocene accords with the present distributions of diploid and tetraploid populations: tetraploid populations of ssp. fuchsii are rare outside Central Europe (Ståhlberg, 2007; Fig. 1).…”

Section: Polyploid Evolution and Phylogeny Of The Dactylorhiza Maculamentioning

confidence: 99%

See 1 more Smart Citation

Evolutionary history of the Dactylorhiza maculata polyploid complex (Orchidaceae)

Ståhlberg¹,

Hedrén²

2010

Biological Journal of the Linnean Society

Self Cite

View full text Add to dashboard Cite

Taxonomic complexity may be associated with migration history and polyploidy. We used plastid and nuclear DNA markers to investigate the evolutionary history of the systematically challenging Dactylorhiza maculata polyploid complex. A total of 1833 individuals from 298 populations from throughout Europe were analysed. We found that gene flow was limited between the two major taxa, diploid ssp. fuchsii (including ssp. saccifera) and tetraploid ssp. maculata. A minimum of three autotetraploid lineages were discerned: (1) southern/western ssp. maculata;(2) northern/eastern ssp. maculata; and (3) Central European ssp. fuchsii. The two ssp. maculata lineages, which probably pre-date the last glaciation, form a contact zone with high genetic diversity in central Scandinavia. Intermediate plastid haplotypes in the contact zone hint at recombination. Central Europe may have been a source area for the postglacial migration for the southern/western lineage of ssp. maculata, as well as for ssp. fuchsii. The northern/eastern lineage of ssp. maculata may have survived the LGM in central Russia west of the Urals. The tetraploid lineage of ssp. fuchsii is indistinguishable from diploid ssp. fuchsii, and is probably of postglacial origin. The Mediterranean region and the Caucasus have not contributed to the northward migration of either ssp. fuchsii or ssp. maculata.

show abstract

Section: Polyploid Evolution and Phylogeny Of The Dactylorhiza Maculamentioning

confidence: 99%

“…fuchsii are common in Central Europe, whereas ssp. maculata is rare or is absent from the same region (Ståhlberg, 2007;Fig. 1).…”

mentioning

confidence: 97%

Evolutionary history of the Dactylorhiza maculata polyploid complex (Orchidaceae)

Ståhlberg¹,

Hedrén²

2010

Biological Journal of the Linnean Society

Self Cite

View full text Add to dashboard Cite

show abstract

“…The number of migrants per generation (N m ) between each pair of populations and population groups was estimated based on the method proposed by Barton and Slatkin () in GENEPOP ver. 4.0 (Rousset ). To investigate the population structure and assign individuals to populations, the Bayesian clustering method was used, as implemented in STRUCTURE ver.…”

Section: Methodsmentioning

confidence: 99%

“…and D. maculata (L.) Soó s.l. (Hedrén , 2003, Hedrén et al , Ståhlberg and Hedrén ). The allotetraploid D. majalis subsp.…”

Section: Introductionmentioning

confidence: 98%

Genetic diversity in Dactylorhiza majalis subsp. majalis populations (Orchidaceae) of northern Poland

Naczk

Ziętara

2019

Nordic Journal of Botany

View full text Add to dashboard Cite

We analysed 16 populations of Dactylorhiza majalis subsp. majalis from northern Poland, simultaneously utilizing both morphological and molecular data. Genetic differentiation was examined using five microsatellite loci, and morphological variation was assessed for 23 characters. At the species level, our results showed a moderate level of genetic diversity (A = 6.00; Ae = 1.86; Ho = 0.387; FIS = 0.139) which varied between the studied populations (A = 2.60–4.20; Ae = 1.68–2.39; Ho = 0.270–0.523; FIS = −0.064–0.355). A significant excess of homozygotes was detected in five population, while excess of heterozygotes was observed in four populations, but the latter values were statistically insignificant. Moderate, but clear between population genetic differentiation was found (FST = 0.101; p < 0.001). Considering pairwise‐FST and number of migrants among populations, we recognized three population groups (I, II, III), where the first could be further divided into two subgroups (Ia, Ib). These three groups differed with respect to gene flow values (Nm = 0.39–1.12). The highest number of migrants per generation was noticed among populations of subgroup Ia (8.58), indicative of a central panmictic population with free gene flow surrounded by peripatric local populations (Ib) with more limited gene flow. Geographic isolation, habitat fragmentation and limited seed dispersal are inferred to have caused limitations to gene flow among the three indicated population groups. There was a significant correlation between the morphological and genetic distance matrices. A weak but significant pattern of isolation by distance was also observed (r = 0.351; p < 0.05).

show abstract

“…and the autotetraploid D. maculata ssp. maculata (Hedrén, , b, c, , b, , ; Hedrén, Fay & Chase, ; Devos et al ., , ; Pillon et al ., ; Ståhlberg & Hedrén, ).…”

Section: Introductionmentioning

confidence: 99%

Plastid DNA haplotype diversity and morphological variation in theDactylorhiza incarnata/maculatacomplex (Orchidaceae) in northern Poland

et al. 2015

View full text Add to dashboard Cite

To gain an overview of the variation in the Dactylorhiza incarnata/maculata complex in northern Poland, ten plastid DNA regions (seven microsatellite and three indel loci) and 23 morphometric characters were used. In total, 972 and 480 samples from 64 and 31 populations were utilized for the genetic and morphometric analyses, respectively. One hundred and forty‐one haplotypes that have not been reported previously were recognized. The continuity of morphological characters between the studied species and the impact of post‐glacial colonization on the observed complexity in the Dactylorhiza incarnata/maculata complex were concluded. It was confirmed that the allotetraploid group of D. majalis s.s. has inherited its plastid genome from D. maculata s.l., specifically from D. maculata ssp. fuchsii. In addition, some of the haplotypes found in D. majalis s.s. were distinct and evidently not present in the preserved D. maculata s.l. Although possible gene flow and introgression between two subspecies of the D. maculata s.l. group were indicated, we suggest that they should be treated as separate evolutionary units. Both the common and rare haplotypes show a similar pattern of geographical distribution for all four taxa analysed, which suggests that hybridization took place relatively recently, shortly after the retreat of the ice sheet. © 2015 The Linnean Society of London, Botanical Journal of the Linnean Society, 2015, 178, 121–137.

show abstract

Systematics and phylogeography of the Dactylorhiza maculata complex (Orchidaceae) in Scandinavia: insights from cytological, morphological and molecular data

Cited by 33 publications

References 56 publications

Evolutionary history of the Dactylorhiza maculata polyploid complex (Orchidaceae)

Evolutionary history of the Dactylorhiza maculata polyploid complex (Orchidaceae)

Genetic diversity in Dactylorhiza majalis subsp. majalis populations (Orchidaceae) of northern Poland

Plastid DNA haplotype diversity and morphological variation in theDactylorhiza incarnata/maculatacomplex (Orchidaceae) in northern Poland

Contact Info

Product

Resources

About