Systemic infection in European perch with thermoadapted virulent <i>Aeromonas salmonicida</i> (<i>Perca fluviatilis</i>)

Rupp, Mélanie; Pilo, Paola; Müller, Barbara; Knüsel, R.; Siebenthal, Beat von; Frey, Joachim; Sindilariu, Paul-Daniel; Schmidt‐Posthaus, Heike

doi:10.1111/jfd.12970

Cited by 11 publications

(6 citation statements)

References 31 publications

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“…EPC cell rounding and rapid apoptosis were detected upon infection with RZ6S‐1 strain (Figure 5a). Consistently, the T3SS‐dependent cell rounding was reported in bluegill fry cells and EPC cells infected with A. salmonicida (Rupp et al., 2019; Vanden & Frey, 2013). However, our study provides sound evidence on the cell apoptosis by detection of cleaved caspase‐3 from infected EPC.…”

Section: Discussionsupporting

confidence: 64%

“…Lesions can range from whitish skin discoloration to deep skin ulcerations up to muscle necrosis (Noga, 2010). In recent years, atypical A. salmonicida strains are being recovered from an increasingly wide range of aquaculture fish, for example black rockfish ( Sebastes schlegeli ) (Han et al., 2011), crucian carp ( Carassius carassius ) (Han et al., 2013), common carp ( Cyprinus carpio ) (Yamin et al., 2017), lumpfish ( Cyclopterus lumpus ) (Rouleau, Vincent, & Charette, 2018) and European perch ( Perca fluviatilis ) (Rupp et al., 2019). Meanwhile, their pathogenesis is still poorly understood and needs further investigation.…”

Section: Introductionmentioning

confidence: 99%

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Pathogenic characterization of Aeromonas salmonicida subsp. masoucida turbot isolate from China

Wang

et al. 2020

Journal of Fish Diseases

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Aeromonas salmonicida is a non-motile gram-negative bacterium that is ubiquitous in aquatic environments. Of its five subspecies, A. salmonicida subsp. salmonicida is known as typical A. salmonicida, which is the most often detected subspecies in diseased fish (Daher et al., 2011). It causes severe septicaemia and acute mortality in susceptible salmonid hosts, whereas the subacute or chronic form of the disease is characterized by the furuncles in the musculature (the sign of furunculosis) (Austin & Austin, 2012). The other four subspecies are referred to as atypical A. salmonicida, and they are subsp. masoucida, subsp. achromogenes, subsp. smithia and subsp. pectinolytica. Their infection leads to atypical furunculosis, except subsp. pectinolytica, which is not a fish pathogen (Wiklund & Dalsgaard, 1998). The primary sign of an atypical A. salmonicida infection is skin ulceration. Lesions can range from whitish skin discoloration to deep skin ulcerations up to muscle necrosis (Noga, 2010). In recent years, atypical A. salmonicida strains are being recovered from an increasingly wide range of aquaculture fish,

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Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Pathogenic characterization of Aeromonas salmonicida subsp. masoucida turbot isolate from China

Wang

et al. 2020

Journal of Fish Diseases

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“…Cell line THP-1 was infected with each of the 24 Aeromonas strains (Table 1) using overnight cultures (18 h) in DMEM without FBS and P/S, at a multiplicity of infection (MOI) of 10 and 20, i.e., the ratio between the number of bacteria and the number of cells targeted (49). The control strain of V. vulnificus (CECT 4999) was used at MOI 5, as done in a previous study (32).…”

Section: Infectionmentioning

confidence: 99%

Immune Response of the Monocytic Cell Line THP-1 Against Six Aeromonas spp.

Fernández-Bravo

Figueras

2022

Front. Immunol.

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Aeromonas are autochthonous bacteria of aquatic environments that are considered to be emerging pathogens to humans, producing diarrhea, bacteremia, and wound infections. Genetic identification shows that 95.4% of the strains associated with clinical cases correspond to the species Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). However, few studies have investigated the human immune response against some Aeromonas spp. such as A. hydrophila, Aeromonas salmonicida, and A. veronii. The present study aimed to increase the knowledge about the innate human immune response against six Aeromonas species, using, for the first time, an in vitro infection model with the monocytic human cell line THP-1, and to evaluate the intracellular survival, the cell damage, and the expression of 11 immune-related genes (TLR4, TNF-α, CCL2, CCL20, JUN, RELA, BAX, TP53, CASP3, NLRP3, and IL-1β). Transcriptional analysis showed an upregulated expression of a variety of the monocytic immune-related genes, with a variable response depending upon the Aeromonas species. The species that produced the highest cell damage, independently of the strain origin, coincidentally induced a higher expression of immune-related genes and corresponded to the more prevalent clinical species A. dhakensis, A. veronii, and A. caviae. Additionally, monocytic cells showed an overexpression of the apoptotic and pyroptotic genes involved in cell death after A. dhakensis, A. caviae, and Aeromonas media infection. However, the apoptosis route seemed to be the only way of producing cell damage and death in the case of the species Aeromonas piscicola and Aeromonas jandaei, while A. veronii apparently only used the pyroptosis route.

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“…Salmonicida (known as typical) , masoucida, achromogenes, pectinolytica , and smithia ( Austin et al., 2007 ; Merino and Tomás, 2016 ; He et al., 2022 ). A. salmonicida has a wide range of hosts, infecting not only infecting salmon and trout ( Du et al., 2015 ), but also Cyprinus carpio ( Maurice and Tinman, 2000 ), Anoplopoma fimbria ( Vasquez et al., 2020 ), Gadusmorhua ( Soto-Dávila et al., 2019 ), Scophthalmus maximus ( Xu et al., 2021 ), and Percafluviatilis ( Rupp et al., 2019 ). The symptoms of infection are mainly “furunculosis” (skin ulcers) and ‘septicemia’ in salmons ( Salomón et al., 2021 ) and C. carpio ( Bhat et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The temperature-dependent expression of type II secretion system controls extracellular product secretion and virulence in mesophilic Aeromonas salmonida SRW-OG1

Chen

Cai

et al. 2022

Front. Cell. Infect. Microbiol.

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Aeromonas salmonicida is a typical cold water bacterial pathogen that causes furunculosis in many freshwater and marine fish species worldwide. In our previous study, the pathogenic A. salmonicida (SRW-OG1) was isolated from a warm water fish, Epinephelus coioides was genomics and transcriptomics analyzed. Type II secretion system was found in the genome of A. salmonicida SRW-OG1, while the expressions of tatA, tatB, and tatC were significantly affected by temperature stress. Also, sequence alignment analysis, homology analysis and protein secondary structure function analysis showed that tatA, tatB, and tatC were highly conservative, indicating their biological significance. In this study, by constructing the mutants of tatA, tatB, and tatC, we investigated the mechanisms underlying temperature-dependent virulence regulation in mesophilic A. salmonida SRW-OG1. According to our results, tatA, tatB, and tatC mutants presented a distinct reduction in adhesion, hemolysis, biofilm formation and motility. Compared to wild-type strain, inhibition of the expression of tatA, tatB, and tatC resulted in a decrease in biofilm formation by about 23.66%, 19.63% and 40.13%, and a decrease in adhesion ability by approximately 77.69%, 80.41% and 62.14% compared with that of the wild-type strain. Furthermore, tatA, tatB, and tatC mutants also showed evidently reduced extracellular enzymatic activities, including amylase, protease, lipase, hemolysis and lecithinase. The genes affecting amylase, protease, lipase, hemolysis, and lecithinase of A. salmonicida SRW-OG1 were identified as cyoE, ahhh1, lipA, lipB, pulA, HED66_RS01350, HED66_RS19960, aspA, fabD, and gpsA, which were notably affected by temperature stress and mutant of tatA, tatB, and tatC. All above, tatA, tatB and tatC regulate the virulence of A. salmonicida SRW-OG1 by affecting biofilm formation, adhesion, and enzymatic activity of extracellular products, and are simultaneously engaged in temperature-dependent pathogenicity.

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Systemic infection in European perch with thermoadapted virulent Aeromonas salmonicida (Perca fluviatilis)

Cited by 11 publications

References 31 publications

Pathogenic characterization of Aeromonas salmonicida subsp. masoucida turbot isolate from China

Pathogenic characterization of Aeromonas salmonicida subsp. masoucida turbot isolate from China

Immune Response of the Monocytic Cell Line THP-1 Against Six Aeromonas spp.

The temperature-dependent expression of type II secretion system controls extracellular product secretion and virulence in mesophilic Aeromonas salmonida SRW-OG1

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