Systemic polymicrobic infection involving <i>Shewanella putrefaciens</i> group in koi

Cocchi, Monia; Zan, Gabrita De; Giusto, Tiziana Di; Deotto, Silvia; Pretto, Tobia; Manfrin, Amedeo; Brunetta, Romina; Toffan, Anna

doi:10.1111/jfd.12892

Cited by 8 publications

(8 citation statements)

References 11 publications

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“…Some species of Shewanella spp. have been isolated frequently from healthy farmed, wild and ornamental fish as well as from diseased fish (Altun et al, 2014;Cocchi et al, 2018;El-Barbary, 2017;Esteve et al, 2017;Hu et al, 2016;Jung-Schroers et al, 2018;Korun et al, 2009;Kozińska & Pękala, 2004;Paździor et al, 2019a;Pękala et al, 2015;Qin et al, 2014;Rusev et al, 2016;Saeed et al, 1987;Saticioglu et al, 2021;Walczak et al, 2017). Skin ulcerative and necrotic lesions are the dominant symptoms of the disease (El-Barbary, 2017;Hu et al, 2016;Jung-Schroers et al, 2018;Korun et al, 2009;Pękala et al, 2015;Rusev et al, 2016;Saeed et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

“…In some cases, Shewanella spp. infections are associated with darkening of the skin, lethargy, mouth erosion, pale gills, lens atrophy and exophthalmia (Altun et al, 2014;Cocchi et al, 2018;Esteve et al, 2017;Korun et al, 2009;Kozińska & Pękala, 2004;Paździor et al, 2019a;Pękala et al, 2015;Pękala-Safińska, 2018;Qin et al, 2014;Saeed et al, 1987;Saticioglu et al, 2021). Internally, haemorrhage in the kidneys and spleen, ascites, pale liver and petechiae in the swim bladder are reported (Altun et al, 2014;El-Barbary, 2017;Esteve et al, 2017;Jung-Schroers et al, 2018;Paździor et al, 2019a;Pękala et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic analysis of Shewanella spp. isolated from fish

Paździor,

Bomba,

Tłuścik

et al. 2023

Journal of Fish Diseases

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Different species of Shewanella spp. widely inhabit freshwater and marine environments. Some of them are opportunistic fish pathogens. The application of high‐throughput sequencing enabled the characterization and taxonomic reclassification of many Shewanella spp. species. Still, some strains collected from fish need to be better recognized. The aim of the present study was to classify and determine the phylogenetic relationships of Shewanella spp. collected from fish. The complete genomes of 94 strains of Shewanella spp. from different fish species were sequenced using Illumina platform (MiSeq). The 16S rRNA gene, genomic features and whole‐genome relationships of those bacteria were comprehensively analysed in comparison to reference strains. Whole‐genome analysis showed that the tested Shewanella spp. strains were clustered into six groups similar to reference strains of S. xiamenensis, S. oneidensis, S. glacialipiscicola, S. hafniensis, S. baltica and S. oncorhynchi. Our study indicates that the whole‐genome sequence analysis enabled taxonomic classification and assessment of the diversity of the Shewanella spp. strains, as opposed to recently the gold standard method of 16S rRNA amplicon sequencing. The high genetic diversity and low similarity to the reference genome of S. oneidensis indicate that the group of strains may be a subspecies or even new species. Furthermore, we showed that the most frequent Shewanella spp. species occurring in freshwater fish in our study is the recently described species S. oncorhynchi.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of Shewanella spp. isolated from fish

Paździor,

Bomba,

Tłuścik

et al. 2023

Journal of Fish Diseases

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“…Shewanella spp ., gammaproteobacteria belonging to the Alteromonadaceae family, are widespread in marine and freshwater environments (Buller, ). Different Shewanella species were isolated from healthy fish as well as from fish exhibiting clinical signs of a disease (Al‐Harbi & Uddin, ; Cocchi et al, ; Esteve, Merchán, & Alcaide, ; Jung‐Schroers et al, ; Kim et al, ; Korun, Akgun‐Dar, & Yazici, ; Pakingking, Palma, & Usero, ; Pękala, Kozińska, Paździor, & Głowacka, ; Qin, Zhu, & Xu, ; Rusev, Rusenova, Simeonov, & Stratev, ; Saeed, Alamoudi, & Al‐Harbi, ). However, contemporary typing methods were not sufficient to determine genotypic diversity of Shewanella fish isolates.…”

Section: Introductionmentioning

confidence: 99%

Genotypic diversity among Shewanella spp. collected from freshwater fish

Paździor

Pękala‐Safińska

Wasyl

2019

Journal of Fish Diseases

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Different Shewanella species are isolated both from healthy and from diseased fish. To date, contemporary methods do not provide sufficient insight to determine species and detail differentiation between tested strains. Bacteria isolated from cultured (n = 33), wild (n = 12) and ornamental (n = 6) fish, as well as several reference strains, were tested by 16S rRNA gene sequencing, ERIC‐PCR and pulsed‐field gel electrophoresis (PFGE) assays. Our study indicates that isolates collected from freshwater fish were genetically diverse. Based on 16S rRNA gene sequences, bacteria were clustered into groups S. putrefaciens, S. xiamenensis and S. oneidensis. Some isolates were classified only to genus Shewanella; thus, 16S rRNA gene analyses were not enough to determine the species. ERIC‐PCR revealed 49 different genotype profiles indicating that the method might be useful for differentiation of Shewanella isolates irrespectively to species identification, contrary to PFGE which is not suitable for Shewanella typing.

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“…On the basis of the bacteriological results, the putative role of Shewanella spp. as a pathogen with a primary role in developing muscular changes was taken into consideration, as also suggested in koi carp showing generalized edema, among other clinical signs [ 40 ]. In this case, the authors supposed that the habitat of these bacteria is probably expanding to different freshwater ecosystems, including ornamental species.…”

Section: Discussionmentioning

confidence: 99%

Multifactorial Causes of Chronic Mortality in Juvenile Sturgeon (Huso huso)

Ciulli

Volpe

Sirri

et al. 2020

Animals

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This investigation focused on an episode of chronic mortality observed in juvenile Huso huso sturgeons. The examined subjects underwent pathological, microbiological, molecular, and chemical investigations. Grossly severe body shape deformities, epaxial muscle softening, and multifocal ulcerative dermatitis were the main observed findings. The more constant histopathologic findings were moderate to severe rarefaction and disorganization of the lymphohematopoietic lymphoid tissues, myofiber degeneration, atrophy and interstitial edema of skeletal epaxial muscles, and degeneration and atrophy of the gangliar neurons close to the myofibers. Chemical investigations showed a lower selenium concentration in affected animals, suggesting nutritional myopathy. Other manifestations were nephrocalcinosis and splenic vessel wall hyalinosis. Septicemia due to bacteria such as Aeromonas veronii, Shewanella putrefaciens, Citrobacter freundii, Chryseobacterium sp., and pigmented hyphae were found. No major sturgeon viral pathogens were detected by classical methods. Next-generation sequencing (NGS) analysis confirmed the absence of viral pathogens, with the exception of herpesvirus, at the order level; also, the presence of Aeromonas veronii and Shewanella putrefaciens was confirmed at the family level by the metagenomic classification of NGS data. In the absence of a primary yet undetected biological cause, it is supposed that environmental stressors, including nutritional imbalances, may have led to immune system impairment, facilitating the entry of opportunistic bacteria and mycotic hyphae.

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Systemic polymicrobic infection involving Shewanella putrefaciens group in koi

Cited by 8 publications

References 11 publications

Phylogenetic analysis of Shewanella spp. isolated from fish

Phylogenetic analysis of Shewanella spp. isolated from fish

Genotypic diversity among Shewanella spp. collected from freshwater fish

Multifactorial Causes of Chronic Mortality in Juvenile Sturgeon (Huso huso)

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