Systemic transcriptome comparison between early‐ And late‐onset pre‐eclampsia shows distinct pathology and novel biomarkers

Guo, Fang; Bao, Zhenan; Yang, Hao; Fu, Yixi; Wang, Yaning; Huang, Jianming; Cheng, Min; Li, Xiaobo; Shen, Zhuo; Li, Li; He, Ping; Xiang, Andy Peng; Wang, Shuaiyu

doi:10.1111/cpr.12968

Cited by 36 publications

(24 citation statements)

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Section: Discussionmentioning

confidence: 99%

“…All above makes DEAPLOG a reliable and attractive approach in scRNA data analysis. Indeed, we have successfully applied it in large-scale human embryonic limb development data analysis and several other applications [37][38][39] . Despite using a completely different framework to identify DEGs, DEAPLOG still returns inflated p-values like many existing methods.…”

Section: Deaplog Can Be Used In Identifying Degs In Spatial Transcrip...mentioning

confidence: 99%

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A method for differential expression analysis and pseudo-temporal locating and ordering of genes in single-cell transcriptomic data

Zhang

2022

Preprint

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Identification of differentially expressed genes (DEGs) is a pivotal step in single-cell RNA sequencing (scRNA-seq) data analysis. The sparsity and multi-model distribution of scRNA-seq data decides that the traditional tools designed for bulk RNA-seq have several limitations when applied to single-cell data. On the other hand, tools specifically for DEGs analysis of scRNA-seq data normally does not consider the high dimensionality of the data. To this end, we present DEAPLOG, a method for differential expression analysis and pseudo-temporal locating and ordering of genes in single-cell transcriptomic data. We show that DEAPLOG has higher accurate and efficient in DEGs identification when compared with existing methods in both artificial and real datasets. Additionally, DEAPLOG can infer pseudo-time and embedding coordinates of genes, therefore is useful in identifying regulators in trajectory of cell fate decision.

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Section: Discussionmentioning

confidence: 99%

Section: Deaplog Can Be Used In Identifying Degs In Spatial Transcrip...mentioning

confidence: 99%

A method for differential expression analysis and pseudo-temporal locating and ordering of genes in single-cell transcriptomic data

Zhang

2022

Preprint

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“…Vitamin D supplementation improves the compliance, elasticity and thickness of the media and intima of vascular structures 44 . Finally, vitamin D deficiency could also be responsible for an increase in blood pressure by acting on the renin angiotensin aldosterone system 45 . Our study might suggest a role for vitamin D, particularly at the end of pregnancy, through the pathophysiological pathways described above.…”

Section: Discussionmentioning

confidence: 99%

“…Vitamin D could especially regulate the maintenance of placental functions. As placental functions are thought to be impaired through a primary involvement of placental cells during early-onset PMCs, but through a secondary involvement due to peripheral blood and vascular cells dysfunctions in late-onset PMCS, vitamin D could mainly act through its systemic, not directly placental effects 45 .…”

Section: Discussionmentioning

confidence: 99%

Vitamin D deficiency during late pregnancy mediates placenta-associated complications

Raia-Barjat

Sarkis

Rançon

et al. 2021

Sci Rep

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During pregnancy, maternal vitamin D insufficiency could increase the risk of preeclampsia. Aim of the study was to evaluate the relationship between vitamin D status and the occurrence of placenta-mediated complications (PMCs) in a population at high risk. A prospective multicenter cohort study of 200 pregnant patients was conducted. The vitamin D level of patients with placenta-mediated complications was lower at 32 weeks compared to uncomplicated pregnancies (P = 0.001). At 32 weeks, the risk of occurrence of PMCs was five times higher in patients with vitamin D deficiency (RR: 5.14 95% CI (1.50–17.55)) compared to patients with normal vitamin D levels. There was a strong, inverse relationship between serum 25(OH)D levels at 32 weeks and the subsequent risk of PMCs (P = 0.001). At 32 weeks, the vitamin D level of patients with late-onset PMCs was lower than the one of patients with early-onset PMCs and of patients without PMCs (P < 0.0001). These results suggest a role of vitamin D in the maintenance of placental performance and therefore in the prevention of the onset of late PMC.

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“…An array of unique fetal cell types derived from the early embryo, including villous cytotrophoblast cells, syncytiotrophoblast cells, extravillous trophoblast cells and maternal immune cells, have been detected [ 98 ]. Taking advantage of this discovery, Guo et al [ 99 ] further compared both placental and peripheral blood transcriptomes to distinguish the fetal and maternal differences between women diagnosed with early- and late-onset PE. Interestingly, classical biomarkers such as sFlt-1, sEng and PAPP-A were only upregulated in the early-onset PE while the maternal blood-derived factors EBI3, IGF2, ORMDL3, GATA2 and KIR2DL4 were identified as new biomarkers for the late-onset PE.…”

Section: Novel Biochemical Markers With Pe Occurrencementioning

confidence: 99%

Diagnostic biomolecules and combination therapy for pre-eclampsia

Chen

et al. 2022

Reprod Biol Endocrinol

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Pre-eclampsia (PE), associated with placental malperfusion, is the primary reason for maternal and perinatal mortality and morbidity that can cause vascular endothelial injury and multi-organ injury. Despite considerable research efforts, no pharmaceutical has been shown to stop disease progression. If women precisely diagnosed with PE can achieve treatment at early gestation, the maternal and fetal outcomes can be maximally optimized by expectant management. Current diagnostic approaches applying maternal characteristics or biophysical markers, including blood test, urine analysis and biophysical profile, possess limitations in the precise diagnosis of PE. Biochemical factor research associated with PE development has generated ambitious diagnostic targets based on PE pathogenesis and dissecting molecular phenotypes. This review focuses on current developments in biochemical prediction of PE and the corresponding interventions to ameliorate disease progression, aiming to provide references for clinical diagnoses and treatments.

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Systemic transcriptome comparison between early‐ And late‐onset pre‐eclampsia shows distinct pathology and novel biomarkers

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 36 publications

References 50 publications

A method for differential expression analysis and pseudo-temporal locating and ordering of genes in single-cell transcriptomic data

A method for differential expression analysis and pseudo-temporal locating and ordering of genes in single-cell transcriptomic data

Vitamin D deficiency during late pregnancy mediates placenta-associated complications

Diagnostic biomolecules and combination therapy for pre-eclampsia

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