SZSJ protects against insomnia by a decrease in ADMA level and an improvement in DDAH production in sleep-deprived rats

Xiao, Hong-Bo; Wang, Yishan; Luo, Zhigang; Lu, Xiangyang

doi:10.1016/j.lfs.2018.07.044

Cited by 23 publications

(10 citation statements)

References 20 publications

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“…Exogenous treatment of human monocytoid cells THP-1 with micromolar concentrations of ADMA significantly upregulates the levels of IL-8, TNF-α, MCP-1, chemokine receptors CCR 2 and CXCR 2 , activation of NF-κB, and promotes monocytic binding to human umbilical vein endothelial cells [57]. ADMA could also be a biomarker for insulin resistance [102] and a sleep disorder caused by misregulation of DDAH expression [103].…”

Section: Adma—the Small Molecule Metabolite Product Of Arginine Mementioning

confidence: 99%

The Biological Axis of Protein Arginine Methylation and Asymmetric Dimethylarginine

Fulton

Brown

Zheng

2019

IJMS

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Protein post-translational modifications (PTMs) in eukaryotic cells play important roles in the regulation of functionalities of the proteome and in the tempo-spatial control of cellular processes. Most PTMs enact their regulatory functions by affecting the biochemical properties of substrate proteins such as altering structural conformation, protein–protein interaction, and protein–nucleic acid interaction. Amid various PTMs, arginine methylation is widespread in all eukaryotic organisms, from yeasts to humans. Arginine methylation in many situations can drastically or subtly affect the interactions of substrate proteins with their partnering proteins or nucleic acids, thus impacting major cellular programs. Recently, arginine methylation has become an important regulator of the formation of membrane-less organelles inside cells, a phenomenon of liquid–liquid phase separation (LLPS), through altering π-cation interactions. Another unique feature of arginine methylation lies in its impact on cellular physiology through its downstream amino acid product, asymmetric dimethylarginine (ADMA). Accumulation of ADMA in cells and in the circulating bloodstream is connected with endothelial dysfunction and a variety of syndromes of cardiovascular diseases. Herein, we review the current knowledge and understanding of protein arginine methylation in regards to its canonical function in direct protein regulation, as well as the biological axis of protein arginine methylation and ADMA biology.

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Section: Adma—the Small Molecule Metabolite Product Of Arginine Mementioning

confidence: 99%

The Biological Axis of Protein Arginine Methylation and Asymmetric Dimethylarginine

Fulton

Brown

Zheng

2019

IJMS

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“…Ziziphi Spinosae Semen (ZSS), known as suan zao ren in China, has been widely used to manage insomnia and palpitations in Traditional Chinese Medicine (Yeung et al, 2012;Rodríguez Villanueva and Rodríguez Villanueva, 2017;Shergis et al, 2017;Zhou et al, 2018). Modern pharmacological research indicates that ZSS exhibits good sedative and hypnotic effects on the central nervous system (Xiao et al, 2018). There are many prescriptions containing ZSS as a raw medical material in the Chinese Pharmacopoeia; these are commonly used to treat palpitations and insomnia.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms Underlying the Action of Ziziphi Spinosae Semen in the Treatment of Insomnia: A Study Involving Network Pharmacology and Experimental Validation

et al. 2021

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Purpose: This study aimed to investigate the potential mechanisms and related bioactive components of ZSS for the treatment of insomnia.Method: The insomnia model of rat induced by PCPA was established. After oral administration of ZSS extract, the general morphological observation, pentobarbital sodium-induced sleep test and histopathological evaluation were carried out. Network pharmacology, assisted by UHPLC-Q-Exactive-MS/MS analysis, was developed to identify the targets of ZSS in the treatment of insomnia, as well as the corresponding signaling pathways. In addition, we validated the identified targets and pathways by RT-qPCR and immunohistochemical analysis.Results: The pentobarbital sodium-induced sleep test, determination of 5-HT and GABA levles in hypothalamic tissues and HE staining showed that ZSS extract was an effective treatment for insomnia. Network pharmacology analysis identified a total of 19 candidate bioactive ingredients in ZSS extract, along with 433 potentially related targets. Next, we performed protein-protein interaction (PPI), MCODE clustering analysis, GO functional enrichment analysis, KEGG pathway enrichment analysis, and ingredient-target-pathway (I-T-P) sub-networks analysis. These methods allowed us to investigate the synergistic therapeutic effects of crucial pathways, including the serotonergic and GABAergic synapse pathways. Our analyses revealed that palmitic acid, coclaurine, jujuboside A, N-nornuciferine, caaverine, magnoflorine, jujuboside B, and betulinic acid, all played key roles in the regulation of these crucial pathways. Finally, we used the PCPA-induced insomnia in rats to validate the data generated by network pharmacology; these in vivo experiments clearly showed that pathways associated with the serotonergic and GABAergic system were activated in the rats model. Furthermore, ZSS treatment significantly suppressed high levels of HTR1A, GABRA1, and GABRG2 expression in the hypothalamus and reduced the expression levels of HTR2A.Conclusion: Based on the combination of comprehensive network pharmacology and in vivo experiments, we successfully identified the potential pharmacological mechanisms underlying the action of ZSS in the treatment of insomnia. The results provide a theoretical basis for further development and utilization of ZSS, and also provide support for the development of innovative drugs for the treatment of insomnia.

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“…There are studies that show that three MADS-box genes (PrMADS1, PrMADS2 and PrMADS3), LEAFY/FLORICAULA and NEEDLY (NLY) were highly participated in the early stages of initiation and differentiation of P. radiata male and female cone buds, as well as vegetative buds [58]. In another study, a transcriptome data analysis showed that the enhanced transcriptional activity of MADS-box transcription factors was also closely related to the formation of early cones, in particular the process of sex reversal [59,60]. Thus, it can be also speculated that MADS-box transcription factors are a key gene family in the molecular mechanism of conifer flowering and seed production.…”

Section: Molecular Mechanismsmentioning

confidence: 96%

An Overview of the Practices and Management Methods for Enhancing Seed Production in Conifer Plantations for Commercial Use

Zhao

et al. 2021

Horticulturae

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Flowering, the beginning of the reproductive growth, is a significant stage in the growth and development of plants. Conifers are economically and ecologically important, characterized by straight trunks and a good wood quality and, thus, conifer plantations are widely distributed around the world. In addition, conifer species have a good tolerance to biotic and abiotic stress, and a stronger survival ability. Seeds of some conifer species, such as Pinus koraiensis, are rich in vitamins, amino acids, mineral elements and other nutrients, which are used for food and medicine. Although conifers are the largest (giant sequoia) and oldest living plants (bristlecone pine), their growth cycle is relatively long, and the seed yield is unstable. In the present work, we reviewed selected literature and provide a comprehensive overview on the most influential factors and on the methods and techniques that can be adopted in order to improve flowering and seed production in conifers species. The review revealed that flowering and seed yields in conifers are affected by a variety of factors, such as pollen, temperature, light, water availability, nutrients, etc., and a number of management techniques, including topping off, pruning, fertilization, hormone treatment, supplementary pollination, etc. has been developed for improving cone yields. Furthermore, several flowering-related genes (FT, Flowering locus T and MADS-box, MCMI, AGAMOUS, DEFICIENCES and SRF) that play a crucial role in flowering in coniferous trees were identified. The results of this study can be useful for forest managers and for enhancing seed yields in conifer plantations for commercial use.

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SZSJ protects against insomnia by a decrease in ADMA level and an improvement in DDAH production in sleep-deprived rats

Cited by 23 publications

References 20 publications

The Biological Axis of Protein Arginine Methylation and Asymmetric Dimethylarginine

The Biological Axis of Protein Arginine Methylation and Asymmetric Dimethylarginine

Mechanisms Underlying the Action of Ziziphi Spinosae Semen in the Treatment of Insomnia: A Study Involving Network Pharmacology and Experimental Validation

An Overview of the Practices and Management Methods for Enhancing Seed Production in Conifer Plantations for Commercial Use

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