T‐cell requirement for establishment of the IgG‐dominant B‐cell lesion in periodontitis

Okada, Hiroshi; Itô, Haruo; Harada, Yasushi

doi:10.1111/j.1600-0765.1987.tb01564.x

Cited by 28 publications

(21 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the cells, Tlymphocytes are known to play important roles in modulating local immune responses at periodontal lesions (Okada et al, 1987(Okada et al, , 1983Ito et al, 1988). Recently, it has been demonstrated that immunocompetent cells express a number of cell adhesion molecules facilitating adhesive cellcell and cell-extracellular matrix (ECM) interactions, and that lymphocyte migration and retention in inflammatory sites are regulated by these molecules (Springer, 1990).…”

Section: Introductionmentioning

confidence: 98%

CD44-Hyaluronate Interaction Participates in the Adherence of T-lymphocytes to Gingival Fibroblasts

et al. 1996

View full text Add to dashboard Cite

It has already been clarified that peripheral blood T-lymphocytes which had been activated with phorbol 12-myristate 13-acetate (PMA) acquired the ability to bind to human gingival fibroblasts (HGF) and that the adherence was mediated by VLA integrins. However, these studies also raised the possibility that molecules other than VLA integrins should be involved in the adherence between T-lymphocytes and HGF. In this study, the possible involvement of CD44, a hyaluronate receptor, in heterotypic cell-cell interactions was investigated. It was confirmed that PMA-activated T-lymphocytes strongly adhered to plate-coated hyaluronate and that the hyaluronate binding was clearly inhibited by the addition of OS/37, a newly established mAb specific for the hyaluronate-binding epitope on CD44. Interestingly, OS/37 also blocked the HGF binding of the activated T-lymphocytes when the adherence to HGF was assessed at 4 degrees C, at which temperature the adhesion of integrin molecules diminished, while that of CD44 functioned normally. Immunofluorescence staining revealed that hyaluronate was anchored along the cell surface of HGF. Furthermore, the binding of activated T-lymphocytes to HGF was significantly inhibited by the treatment of HGF with hyaluronidase. These results clearly demonstrated that CD44-hyaluronate interactions participated at least in part in the adhesiveness of T-lymphocytes to HGF.

show abstract

Section: Introductionmentioning

confidence: 98%

CD44-Hyaluronate Interaction Participates in the Adherence of T-lymphocytes to Gingival Fibroblasts

et al. 1996

View full text Add to dashboard Cite

show abstract

“…B cells and T cells accumulate in large numbers in the periodontal tissues, although until recently we have had little information on cellular synthetic activity and proliferation of these cells. Tissue culture experiments have shown that T cells are involved in the modulation of immunoglobulin synthesis [3–5]. However, the results of these studies do not easily extrapolate to the in vivo situation where complex interactions between a variety of cells of the inflammatory infiltrate occur.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory cytokine IL-10 and T cell cytokine profile in periodontitis granulation tissue

Lappin

Macleod

Kerr

et al. 2001

Clinical and Experimental Immunology

131

111

View full text Add to dashboard Cite

SUMMARYTh2 cells are more abundant than Th1 cells in periodontitis lesions, but the relative importance of the Th1 and Th2 subsets in periodontal disease is not understood. In addition, the role of proinflammatory and anti-inflammatory cytokines in this disease process is unclear. Biopsies were obtained from 10 patients with early onset periodontitis (EOP) and 10 patients with adult periodontitis (AP). From all of the patients in the AP group we were able to obtain and section the gingival tissue to serve as controls. We used polyclonal monospecific antibodies to detect cells expressing IL-2, IL-4, IL-6, IL-10 and IL-15, tumour necrosis factor (TNF-a ) and interferon-gamma (IFN-g) in formalin-fixed, paraffin-embedded sections of granulation tissue from periodontitis lesions. We also employed a series of oligonucleotide probes to detect cells expressing the cytokine transcripts in the same tissue biopsies. Cells that expressed IL-4 or IL-6 were more numerous than cells expressing either IL-2 or IFN-g. Th2 cells were more numerous in EOP and AP tissues. IL-15 substitutes for IL-2 in a number of biological activities related to the Th1 immune response, and interestingly, in periodontal lesions the IL-15-expressing cells outnumbered IL-2-expressing cells, suggesting that this is the pattern of immune regulation by T cells in the periodontium. The functional balance in the T cell subsets detected by their cytokine profiles underlies the importance of the anti-inflammatory mechanisms taking place in the diseased tissue. The numbers of inflammatory leucocytes that express the anti-inflammatory cytokine IL-10 are much more widely distributed than those that express the proinflammatory cytokines IL-6 and TNF-a . This study suggests that large numbers of infiltrating inflammatory cells as well as accessory cells are involved in the down-regulation of the inflammatory and immune response in periodontitis.

show abstract

“…In recent years it has been shown that macrophages and lymphocytes are not a homologous cell population but can be differentiated into biologically active subpopulations. Therefore, using monoclonal antibodies, different subsets of T cells could be identified in the inflammatory infiltrate of gingivitis (5,6) and periodontitis lesions (7,8,9,10,11).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic dynamics of macrophage subpopulations during human experimental gingivitis

Topoll

Zwadlo

Lange

et al. 1989

J of Periodontal Research

View full text Add to dashboard Cite

The purpose of the present study was to investigate whether functionally different macrophages are present in clinically healthy gingiva and during human experimental gingivitis. Eight male probands were introduced to an oral hygiene program until all reached mean Plaque and Gingival Index scores approaching zero. During the following 19 days all oral hygiene was abandoned. At d -14, 0, 2, 4, 7, 11 and 19 clinical indices and gingival biopsies were taken. Cryostat sections were incubated with monoclonal antibodies against mature macrophages (25F9), inflammatory macrophages (27E10) and anti-inflammatory macrophages (RM 3/1). Positive cells were counted in the inflammatory infiltrate (IF) and the connective tissue (CT). At d -14 elevated numbers of 27E10-positive cells were observed which decreased significantly at d 0 (p less than 0.018) and increased again at d 19 (p less than 0.026). Significant differences in the number of RM 3/1-positive cells were found between d 0 and d -14, 2, 4 and 7 (p less than 0.05) while no differences in the number of 25F9-positive cells were observed throughout this study. It was concluded that experimental gingival inflammation is characterized by the appearance and disappearance of functionally different macrophage subpopulations.

show abstract

T‐cell requirement for establishment of the IgG‐dominant B‐cell lesion in periodontitis

Cited by 28 publications

References 9 publications

CD44-Hyaluronate Interaction Participates in the Adherence of T-lymphocytes to Gingival Fibroblasts

CD44-Hyaluronate Interaction Participates in the Adherence of T-lymphocytes to Gingival Fibroblasts

Anti-inflammatory cytokine IL-10 and T cell cytokine profile in periodontitis granulation tissue

Phenotypic dynamics of macrophage subpopulations during human experimental gingivitis

Contact Info

Product

Resources

About