T lymphocytes increase the synthesis of esterified cholesterol in human monocyte-derived macrophages by activation of the scavenger pathway

Kotake, Hidetoshi; Oikawa, Shinichi; Naito, Takashi; Hayasaka, Kazuki; Toyota, Takayoshi

doi:10.1016/0925-4439(92)90011-b

Cited by 7 publications

(2 citation statements)

References 27 publications

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“…Taken together, those investigators suggest that T lymphocyte-macrophage interactions may reduce intracellular cholesterol accumulation in the atherosclerotic lesion. In contrast Kotake et al [19] have recently provided evidence that T lymphocytes activated by coculture with macrophages can produce a substance that increases the synthesis of esterified cholesterol in macrophages by activation of the scavenger pathway.…”

Section: Discussionmentioning

confidence: 96%

Participation of T lymphocytes in atherogenesis: sequential and quantitative observation of aortic lesions of rats with diet-induced hypercholesterolaemia using en face double immunostaining

Haraoka

Shimokama

Watanabe

1995

Vichows Archiv A Pathol Anat

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Using en face double immunostaining coupled with electron microscopy, we studied the temporal and spatial distribution of T lymphocytes and macrophages during the development of atherosclerosis in a diet-induced rat model fed an atherogenic diet for 2-40 weeks. T lymphocytes and macrophages adhered to the aortic surface by 2 weeks on the diet, with subsequent migration under the endothelium, and formed a fatty streak-like lesion. Analysis of the cellular components revealed that infiltration of T lymphocytes was most prominent in the incipient phase of lesion formation accounting for 60%, 29% and 34% of mononuclear cells appearing in 2-week lesions of the superior thoracic, inferior thoracic and abdominal segments of the aorta, respectively. After the incipient phase, the relative number of T lymphocytes in the three segments of the aorta showed a slow decline; the proportion of T lymphocytes to macrophages was approximately 1:3 to 1:4 in 10- to 20-week lesions. An overall view of the lesional cells often demonstrated direct cellular contact between T lymphocytes and macrophages. Further, OX6/ED1 double immunostaining demonstrated that Ia antigen was expressed on most macrophages. In later stages, breakdown of foamy macrophages occurred, and the extracellular accumulation of lipids and cell debris became prominent. The results demonstrated that in the diet-induced rat model, together with macrophages, large numbers of T lymphocytes participated in all stages of aortic lesions, initially adhering to the surface at prelesional stages and later as the principal component of the atherosclerotic lesion. It is possible that the method described here will provide a good tool for examining the role of T lymphocytes in atherogenesis.

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Section: Discussionmentioning

confidence: 96%

Participation of T lymphocytes in atherogenesis: sequential and quantitative observation of aortic lesions of rats with diet-induced hypercholesterolaemia using en face double immunostaining

Haraoka

Shimokama

Watanabe

1995

Vichows Archiv A Pathol Anat

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“…Observations by image analysis that intimal Mφs in rnu/rnu rats increased in size more rapidly imply that depletion of T cells accelerated foamy transformation of Mφs in the intima. To date in vitro studies suggest that products of activated T cells, such as interferon γ, could modulate Mφs function to accumulate modified (or oxidized) lipoproteins via scavenger receptors as Mφs differentiate into foam cells 4–6 . However, reports about the role of T cells in lipoprotein metabolism in atherosclerosis are still controversial, perhaps because of the use of different experimental conditions in the various studies in vitro.…”

Section: Discussionmentioning

confidence: 99%

Role of T Lymphocytes in the Pathogenesis of Atherosclerosis: Animal Studies Using Athymic Nude Rats

Haraoka

Shimokama

Watanabe

1997

Annals of the New York Academy of Sciences

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Serotonin-stimulated aortic endothelial cells secrete a novel T lymphocyte chemotactic and growth factor

Katz

Farber

Dodds-Stitt

et al. 1994

Journal of Leukocyte Biology

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Atherosclerotic lesions contain multiple cell types including smooth muscle cells, macrophages, and T lymphocytes. The development of an extralymphatic T lymphocyte focus of inflammation in this condition requires chemoattractant-induced cell migration and growth factor-induced cell activation. In a previous study, we described a novel 13-15-kDa T lymphocyte-specific chemotactic cytokine, endothelial cell-derived lymphocyte chemoattractant activity (ED-LCA), secreted by serotonin-stimulated bovine aortic endothelial cells that is distinct from previously identified endothelial cell-derived interleukins (IL) 1, 6, and 8. Because of the association between T lymphocyte chemotactic and growth factor activity, in the current study we investigated the effect of ED-LCA on T cell growth. We assessed its capacity to induce markers of the passage of T cells from the resting (G0) state into the G1 phase of the cell cycle, such as receptors for IL-2 (IL-2R) and transferrin (TFR) and class II major histocompatibility complex antigens (HLA-DR). Incubation of G0 freshly isolated human T lymphocytes for 48 h with chromatographically resolved, partially purified ED-LCA resulted in a threefold increase in expression of the p55 subunit of IL-2R, a threefold increase in TFR, and a twofold increase in HLA-DR. Passage into the G1 phase of the cell cycle was confirmed by cell cycle analysis employing acridine orange. Evaluation of CD4+ and CD8+ T cell subsets by double-antibody labeling demonstrated that the p55 subunit of IL-2R was induced in both T cell subsets. Although incubation of human T cells with ED-LCA alone did not induce proliferation, addition of exogenous IL-2 to T cells pulsed with ED-LCA for 24 h caused a proliferative response with a stimulation index of 3. By up-regulating functional cell surface receptors for IL-2, ED-LCA is a competence growth factor for T lymphocytes and primes them to respond to IL-2. By virtue of its effect on T cells, as a chemotactic and competence factor, this endothelial cell-derived mitoattractant could participate with other T cell growth factors like IL-2 in the recruitment and amplification of the extralymphatic T cell component of atherosclerosis.

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T lymphocytes increase the synthesis of esterified cholesterol in human monocyte-derived macrophages by activation of the scavenger pathway

Cited by 7 publications

References 27 publications

Participation of T lymphocytes in atherogenesis: sequential and quantitative observation of aortic lesions of rats with diet-induced hypercholesterolaemia using en face double immunostaining

Participation of T lymphocytes in atherogenesis: sequential and quantitative observation of aortic lesions of rats with diet-induced hypercholesterolaemia using en face double immunostaining

Role of T Lymphocytes in the Pathogenesis of Atherosclerosis: Animal Studies Using Athymic Nude Rats

Serotonin-stimulated aortic endothelial cells secrete a novel T lymphocyte chemotactic and growth factor

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