T24 HRAS transformed NIH/3T3 mouse cells (GhrasT-NIH/3T3) in serial tumorigenic in vitro/in vivo passages give rise to increasingly aggressive tumorigenic cell lines T1-A and T2-A and metastatic cell lines T3-HA and T4-PA

Ray, Durwood B.; Merrill, Gerald A.; Brenner, Frederic J.; Lytle, Laurie S.; Lam, Tan; McElhinney, Aaron; Anders, Joel; Rock, Tara Tauber; Lyker, Jennifer Kier; Barcus, S.; Leslie, Kara Hust; Kramer, Jill M.; Rubenstein, Eric M.; Schanz, Karen Pryor; Parkhurst, Amy J.; Peck, Michelle A.; Good, Kimberly; Granath, Kristi Lemke; Cifra, Nicole; Detweiler, Jessalee Wantz; Stevens, Laura E.; Albertson, Richard; Deir, Rachael; Stewart, E.A; Wingard, Katherine; Richardson, Micah Rose; Blizard, Sarah; Gillespie, Lauren E.; Kriley, Charles E.; Rzewnicki, Daniel; Jones, David H.

doi:10.1016/j.yexcr.2015.07.029

Cited by 3 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These cells were kindly provided by Durwood B. Ray and their origins, growth properties, morphologies, and tumorigenic capabilities have been described previously. The following description of cell lines and methodology was based on Ray et al (2015). The NIH Swiss mouse embryonic non-transformed cells were produced as described by Todaro and Green (1963) by establishing primary cultures from eight whole 17 to 19 day old NIH Swiss mouse embryos.…”

Section: Nih Swiss Mouse Embryonic Cellsmentioning

confidence: 99%

Effect of Mansoa alliacea (Bignonaceae) leaf extract on embryonic and tumorigenic mouse cell lines

Towne¹,

Dudt²,

Ray³

2015

J. Med. Plants Res.

View full text Add to dashboard Cite

Mansoa alliacea contains high concentrations of chemicals thought to be associated with the suppression of tumor growth. Additionally, this plant has been reported to possess analgesic, antifungal, and anti-bacterial properties, thereby providing other potential benefits for cancer patients. Low doses of a water extract of M. alliacea were applied to a cancerous and non-cancerous cell line. Doses between 1.254 to 10.04 mg/ml of extract applied to T3-HA cancer cells inhibited cell growth, but higher doses of 29.92 to 89.6 mg/ml destroyed colonies of the cancer cells. Application of the extract to NIH Swiss mouse cell cultures resulted in the inhibition of growth at higher concentrations, but at a concentration of 10.14 mg/ml, cell growth began to increase after three days. However, cell death was less at lower concentrations than that of T3-HA cancer cells, thereby confirming that lower concentrations of Ajo de Monte will inhibit cancer cell growth as well as initially inhibit non-cancer cells. Thus, M. alliacea extract selectively targets T3-HA mouse cancer cells but not NIH Swiss embryonic mouse cells. Future research may consider the use of this plant for human cancer patients.

show abstract

Section: Nih Swiss Mouse Embryonic Cellsmentioning

confidence: 99%

Effect of Mansoa alliacea (Bignonaceae) leaf extract on embryonic and tumorigenic mouse cell lines

Towne¹,

Dudt²,

Ray³

2015

J. Med. Plants Res.

View full text Add to dashboard Cite

show abstract

Embedded 3D Printing of Ultrasound‐Compatible Arterial Phantoms with Biomimetic Elasticity

Chee

Golzar

et al. 2022

Adv Funct Materials

View full text Add to dashboard Cite

Here, a class of ink materials and an embedded 3D printing strategy for the fabrication of macroscale elastic tissue‐mimetic constructs are presented. Novel inks composed of 10 wt% glycidyl methacrylated poly(vinyl alcohol) (PVAGMA) with different degrees of substitution (DOS) and 4 wt% cellulose nanocrystals (CNCs) (PVAGMA(DOS)/CNC) with strong shear‐thinning property are developed. By controlling the DOS of PVAGMA, hydrogels with desired mechanical stiffness mimicking that of healthy and diseased artery are designed to construct vascular phantoms. Cyclic tensile tests and in vitro hemodynamic study performed on phantoms demonstrate their excellent mechanical stability and low hysteresis. The burst pressure is found to be about 273 mmHg for PVAGMA2/CNC and 102 mmHg for PVAGMA4/CNC and the printed vascular phantoms are able to withstand 863K cycles over 10 days. Further, their suitability for ultrasonic imaging is demonstrated. Via B‐mode imaging, it is found that the vessel strains under a pulsatile flow are 11.7 ± 1.0% and 6.5 ± 1.5% for PVAGMA2/CNC and PVAGMA4/CNC vessels, respectively, perfectly matching the behaviors of healthy and atherosclerotic arteries. It exemplifies that the ink materials and printing strategy can have broad applications in biomedical research, especially for the fabrication of complex elastic tissue phantoms.

show abstract

In vitro Characterization of a novel murine model of cancerous progression

Scahill

Sherman

Guidry

et al. 2023

Advances in Cancer Biology - Metastasis

View full text Add to dashboard Cite

T24 HRAS transformed NIH/3T3 mouse cells (GhrasT-NIH/3T3) in serial tumorigenic in vitro/in vivo passages give rise to increasingly aggressive tumorigenic cell lines T1-A and T2-A and metastatic cell lines T3-HA and T4-PA

Abstract: T24 HRAS transformed NIH/3T3 mouse Cells (GhrasT-NIH/3T3) in serial tumorigenic in vitro/in vivo Passages give rise to Increasingly aggressive tumorigenic cell Lines T1-A and T2-A and metastatic cell Lines T3-HA and T4-PA.,

Cited by 3 publications

References 36 publications

Effect of Mansoa alliacea (Bignonaceae) leaf extract on embryonic and tumorigenic mouse cell lines

Effect of Mansoa alliacea (Bignonaceae) leaf extract on embryonic and tumorigenic mouse cell lines

Embedded 3D Printing of Ultrasound‐Compatible Arterial Phantoms with Biomimetic Elasticity

In vitro Characterization of a novel murine model of cancerous progression

Contact Info

Product

Resources

About