T4 lysozyme fused with cellulose-binding module for antimicrobial cellulosic wound dressing materials

Abstract

The spread of antibiotic resistance requires innovative strategies for discovery and development of effective antimicrobial alternatives. This report presents a novel strategy for producing antimicrobial wound dressing materials.

Complete Genome Sequence of vB_EcoM-UFV13, a New Bacteriophage Able To Disrupt Trueperella pyogenes Biofilm

“…Notable is the presence of ORF 130, which encodes a glycoside hydrolase (family 24), an enzyme able to act in a Gram-positive biofilm and cause lysis in a Gram-negative bacteria. This property was also observed by the authors of reference (8). A gene cluster, without introns or pseudogenes, encoding 10 tRNAs (Gln, Leu, Gly, Pro, Ser, Thr, Met, Tyr, Asn, and Arg) was identified using tRNAscan-SE (5, 9).…”

A Review on Surface-Functionalized Cellulosic Nanostructures as Biocompatible Antibacterial Materials

“…Because of its efficiency and its specificity against harmful bacteria, the phage is a promising potential contender to replace traditional synthetic antibiotics [167,168]. Bacteriophages are usually negatively charged at their head and thus can bond with positively charged particles through electrostatic interaction [169][170][171][172][173]. In addition, the active groups on the phage such as amino and carboxylic acid groups allow for functionalization through covalent attachment, resulting in a much more durable attachment.…”

Sequence-function Relationships in Phage-encoded Bacterial Cell Wall Lytic Enzymes and their Implications for Phage-derived Products Design

“…Such strategies enable the design and production of tailor-made antimicrobials, based on the conjunction of diverse functions of interest into a single protein. Functions of interest may include, besides a catalytic activity against the peptidoglycan network (i.e., an antimicrobial activity), an increased stability in complex media (25) or, more typically, a certain tropism towards a specific element on the bacterial surface (26) or some other macromolecules like cellulose (27). The engineering approaches mentioned above have circumvented the alleged inability of lysins to cross the outer membrane (OM) of G− (28,29).…”