T98G Cell Death Induced by Photothermal Treatment with Hollow Gold Nanoshell-Coupled Silica Microrods Prepared from Escherichia Coli

Abstract

As an alternative to traditional cancer treatment, photothermal therapy is a promising method with advantages such as noninvasiveness and high efficiency. Herein, we synthesized armored golden Escherichia coli (AGE) microrods as photothermal agents to evaluate the viability of cancer cell. The hollow gold nanoshell (HAuNS) was synthesized for photothermal effects under the near-infrared (NIR) region using unicellular E. coli as a framework. Coupling HAuNS onto the surface of E. coli@SiO2 enhanced temperature elevation and resulted in high conversion efficiency. The synthesized AGE microrods had excellent photothermal stability under NIR laser irradiation in the five-times recycling experiment. The temperature elevation of AGE microrod solution reached 43.7 °C, which induced hyperthermia-mediated killing of tumor cells. The results of the cytotoxicity test revealed the AGE microrod-induced T98G cell death mediated via apoptosis.

Photothermal-Mediated Catalytic Reduction of 4-Nitrophenol Using Poly(N-isopropylacrylamide-acrylamide) and Hollow Gold Nanoparticles

“…In this study, a core-satellite structure was prepared, rather than a core–shell structure, to enhance the photothermal-mediated catalytic performance of as-made materials with different interdistance between satellite particles (HAuNPs) by the LSPR-coupling effect. In our previous study, gold-spiked silica microrods based on Escherichia coli, which has a core-satellite structure, were prepared and used for photothermal therapy. , The core-satellite structure not only improved the photothermal performance but also allowed the adjustment of the interval between satellite particles . Because of thermoresponsive properties as the LCST-polymer, the core contracted and thus the distance between the satellite particles on the core was changed with increasing temperature.…”

“…HAuNPs were prepared by the galvanic replacement reaction using AgNPs and HAuCl 4 . AgNPs were prepared by mixing 10 mL of 25 mM silver nitrate and 10 mL of trisodium citrate (34 mM) with 80 mL of boiled DI water.…”

“…Metal nanoparticles (NPs), especially gold NPs (AuNPs), have received significant attention for driving chemical reactions owing to their excellent light absorption properties . This distinctive feature of AuNPs is their localized surface plasmon resonance (LSPR), which is induced by collective excitations of the free electrons in the AuNPs with light irradiation . In addition, LSPR-mediated photothermal heating under near-infrared (NIR) irradiation, which is a non-radiative property of AuNPs, has shown potential for photothermal therapy and for the enhancement of chemical reactions .…”

Inorganic Nanomaterials for Photothermal‐Based Cancer Theranostics

“…PCE is the parameter used to evaluate the photothermal performance of the inorganic NPs. [132] By applying this parameter, it is possible to compare the ability of different NPs to convert light into heat, if one has knowledge of the absorbance of their suspension at a specific wavelength. It is essential to highlight the fact that PCEs are generally not comparable at different wavelengths due to the nonlinear dependence of the absorption coefficient.…”

Role of Solvent in Electron‐Phonon Relaxation Dynamics in Core‐Shell Au−SiO2 Nanoparticles

“…Metal nanoparticles have generated significant interest in recent times because of their unique optical, electrical and photocatalytic properties. [1][2][3][4][5][6][7][8][9] They have a myriad of applications, e. g. in targeted drug delivery, [10][11][12] bio imaging, [13][14][15][16] biosensing, [17,18] radiative decay engineering, [19] directional fluorescence [20] as nanothermometers, [21][22][23][24] in control of DNA hybridization [25] and in enhancement of emission of biomolecules. [7,16,18,26] Dynamics of heat dissipation, electron dynamics and relaxation processes often play crucial roles in these applications.…”