Tackling of Renal Carcinogenesis in Wistar Rats by Silybum marianum Total Extract, Silymarin, and Silibinin via Modulation of Oxidative Stress, Apoptosis, Nrf2, PPARγ, NF‐κB, and PI3K/Akt Signaling Pathways

Yassin, Nour Y. S.; AbouZid, Sameh; El-Kalaawy, Asmaa M.; Ali, Tarek M.; Elesawy, Basem H.; Ahmed, Osama M.

doi:10.1155/2021/7665169

Cited by 26 publications

(12 citation statements)

References 101 publications

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“…To explore the mechanism of silibinin inhibiting oxidative stress, we tested Nrf2, a key factor regulating oxidative stress homeostasis, and ADH3 and ALDH2, two key enzymes regulating formaldehyde metabolism. In the literature related, silibinin activated Nrf2 signaling to modulated oxidative stress [ 64 , 65 ], and we showed similar results that silibinin increased Nrf2 nuclear translocation and transcription of downstream genes in FA-treated mice. Moreover, Nrf2 regulates the ALDH2 expression, and interestingly, ALDH2 can increase the Nrf2 expression, suggesting an NRF2-ALDH2 feedback loop [ 66 – 68 ].…”

Section: Discussionsupporting

confidence: 82%

Silibinin Ameliorates Formaldehyde-Induced Cognitive Impairment by Inhibiting Oxidative Stress

Wei

Wang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Silibinin is a flavonoid extracted from the medicinal plant Silybum marianum (milk thistle), traditionally used to treat liver disease. Recent studies have shown that the antioxidative stress and anti-inflammatory effects of milk thistle are used in the treatment of neurological diseases. Silibinin has antioxidative stress and antiapoptotic effects and reduces cognitive impairment in models of Alzheimer’s disease (AD). However, the underlying mechanism of silibinin related to improvement of cognition remains poorly understood. In this study, we used the model of lateral ventricle injection of formaldehyde to examine the related mechanism of silibinin in improving cognitive impairment disorders. Oral administration of silibinin for three weeks significantly attenuated the cognitive deficits of formaldehyde-induced mice in a Y -maze test and Morris water maze test. Y -maze results show that silibinin increases the rate of spontaneous response alternation in FA-induced mice. Silibinin increases the target quadrant spending time and decreases escape latency in the Morris water maze test. We examined the effect of silibinin on the NRF2 signaling pathway, and silibinin promoted the nuclear transfer of NRF2 and increased the expression of HO-1 but did not significantly increase the protein expression of NRF2 in the hippocampus. Well, silibinin reduces the content of DHE and decreases the levels of apoptosis of mature neuron cells. We investigated the effect of silibinin on the content of formaldehyde degrading enzymes; biochemical analyses revealed that silibinin increased GSH and ALDH2 in formaldehyde-induced mice. In addition, as one of the pathological changes of AD, TAU protein is also hyperphosphorylated in FA model mice. Silibinin inhibits the expression of GSK-3β in model mice, thereby reducing the phosphorylation of TAU proteins ser396 and ser404 mediated by GSK3β. Based on our findings, we verified that the mechanism of silibinin improving cognitive impairment may be antioxidative stress, and silibinin is one of the potentially promising drugs to prevent formaldehyde-induced cognitive impairment.

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Section: Discussionsupporting

confidence: 82%

Silibinin Ameliorates Formaldehyde-Induced Cognitive Impairment by Inhibiting Oxidative Stress

Wei

Wang

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…43 In various studies conducted, it was reported that silibinin was effective in treating arsenic-induced nephrotoxicity by eliminating oxidative stress, inflammation, and apoptosis in rats, and that silibinin also activated GST enzyme activity. 44 In the study conducted by Yassin et al (2021), in which they examined the effectiveness of silymarin and silibinin against experimentally induced renal carcinogenesis in male Wistar rats, it was reported that silymarin and silibinin application weakened toxicity indicators in serum, decreased lipid peroxidation, considerably strengthened renal antioxidant arsenal, as well as increased GST enzyme activity and decreased GR enzyme activity. 45 In vitro experiments showed that silibinin nanoparticles protect liver cells and reduce cellular damage.…”

Section: Discussionmentioning

confidence: 99%

“…44 In the study conducted by Yassin et al (2021), in which they examined the effectiveness of silymarin and silibinin against experimentally induced renal carcinogenesis in male Wistar rats, it was reported that silymarin and silibinin application weakened toxicity indicators in serum, decreased lipid peroxidation, considerably strengthened renal antioxidant arsenal, as well as increased GST enzyme activity and decreased GR enzyme activity. 45 In vitro experiments showed that silibinin nanoparticles protect liver cells and reduce cellular damage. This was shown by decreased serum ALP, ALT, and AST levels.…”

Section: Discussionmentioning

confidence: 99%

In Vivo and in Vitro Regulatory Effect of Silibinin on Some Metabolic Enzyme Activities against Cobalt Induced Toxicity in Rats: A Biochemical Approach

Temel,

Akkoyun,

Akkoyun

et al. 2023

ACS Omega

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“…Milk thistle accumulates essential biological elements, namely selenium and cuprum, which -together with vitamin E -stimulate the formation of antibodies and strengthen the immune system of the organism, and also activate the system of antioxidative protection (Yassin et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Effect of liposomal drug based on interferon and extract from Silybum marianum on antioxidative status of bulls against the background of contamination of fodders by cadmium and plumbum

Gutyj

Martyshuk

Парченко

et al. 2022

Regul. Mech. Biosyst.

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The balance between anti- and prooxidants in animal organisms in general and in each cell in particular is responsible for the regulation of many metabolic processes that provide immunocompetence, growth, development and protection of animals from oxidative stress, related to inflow of cadmium and plumbum. Therefore, the objective of our study was the influence of a liposomal drug based on interferon and milk thistle (Silybum marianum (L.) Gaertn.) on the antioxidative status of the organism of bulls in the conditions of cadmium and plumbum loading. The experiments were performed on six-months-old Black Motley dairy cattle. The fodders in the farm were determined to contain high levels of plumbum and cadmium. The liposomal drug Lipointersyl inhibited the lipid peroxidation processes in the bulls. The drug components promoted the decrease in the level of intermediate and end products of lipid peroxidation, in particular 22% decrease in the level of diene conjugates and 20% decrease in TBA-active products. Intramuscular injection of the liposomal drug to bulls of the experimental group strengthened the antioxidant protection of their organism. On the 30th day of the experiment, blood from experimental group animals was seen to have a 9.8% increase in reduced glutathione. Assay of the enzymatic link of the glutathione system revealed that the activity of glutathione peroxidase and glutathione reductase in the blood of the animals that had been injected the liposomal drug Lipointensyl had increased by 24.0% and 27.7% respectively by the 30th day of the experiment. The experiments conducted on young cattle demonstrated that intermuscularly injected the Lipointensyl liposomal drug – against the background of cadmium and plumbum loading – promoted the activation of the glutathione system of antioxidant protection as a result of increase in the activity of its enzymatic and non-enzymatic links. The study of catalase and superoxide dismutase activities revealed that on the 30th and 40th days of the experiment, the activity of those enzymes varied within the physiological norms. Therefore, the analyzed Silybum marianum-based liposomal drug has antioxidant properties, it is recommended for young cattle in the conditions of contamination with heavy metals in order to prevent the development of oxidative stress.

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Tackling of Renal Carcinogenesis in Wistar Rats by Silybum marianum Total Extract, Silymarin, and Silibinin via Modulation of Oxidative Stress, Apoptosis, Nrf2, PPARγ, NF‐κB, and PI3K/Akt Signaling Pathways

Cited by 26 publications

References 101 publications

Silibinin Ameliorates Formaldehyde-Induced Cognitive Impairment by Inhibiting Oxidative Stress

Silibinin Ameliorates Formaldehyde-Induced Cognitive Impairment by Inhibiting Oxidative Stress

In Vivo and in Vitro Regulatory Effect of Silibinin on Some Metabolic Enzyme Activities against Cobalt Induced Toxicity in Rats: A Biochemical Approach

Effect of liposomal drug based on interferon and extract from Silybum marianum on antioxidative status of bulls against the background of contamination of fodders by cadmium and plumbum

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