Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides

Zhang, Xihao; Wang, Jiewen; He, Xiwen; Chen, Langxing; Zhang, Yukui

doi:10.1021/acsami.5b06445

Cited by 148 publications

(54 citation statements)

References 61 publications

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“…There were still some amino residues on the surface of the modified particles that will provide -B-N-'teamed affinity' to extend the application in a lower pH environment. 27,28 Overall, we could therefore draw the conclusion that DFFPBA-agarose gel particles were successfully synthesized.…”

Section: Preparation and Characterization Of Dffpba-agarose Gel Partimentioning

confidence: 89%

Boronic acid‐functionalized agarose affinity chromatography for isolation of tropomyosin in fishes

et al. 2019

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BACKGROUND Tropomyosin is now receiving increasing attention because of its significant allergenic activity in various fishery products but its simple and effective isolation still remains a challenging task. RESULTS An agarose‐based boronate affinity chromatography was produced for the first time to isolate tropomyosin in various fishery products using 3,5‐difluoro‐4‐formyl‐phenylboronic acid as the functional monomer, tris(2‐aminoethyl)amine as the multi‐branched ligand, and agarose gel particles as supporting materials. The agarose concentration, binding pH, and the concentration of elution buffers demonstrated significant effects on separation performance. Under optimized conditions, the purity of the isolated tropomyosin was higher than 90%, with the column adsorption capacity over 1.85 mg mL−1 and the enrichment efficiency over 65%. Such efficiency was also validated with different fish samples including Paralichthys olivaceus, Thunnusthynnus, Oreochromis spp., and Lophius litulon. CONCLUSION In comparison with conventional methods, the established affinity chromatography demonstrated excellent biocompatibility (without involving any organic solvent), better speed (from at least 1–2 days to 3–4 h), and simplicity (from at least five steps to three steps). This suggests that it is a novel and promising technique for the isolation of tropomyosin and other glycoproteins (including most allergens) in foodstuffs. © 2019 Society of Chemical Industry

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Section: Preparation and Characterization Of Dffpba-agarose Gel Partimentioning

confidence: 89%

Boronic acid‐functionalized agarose affinity chromatography for isolation of tropomyosin in fishes

et al. 2019

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“…Moreover, hydrophilic boronic acid nanomaterials were fabricated through the polymerization of boronic acid monomer and hydrophilic monomer, which was expected to exert synergistic effect that could enhance the capture performance. Zhang et al fabricated a mound of Fe 3 O 4 @P(AAPBA‐co‐monomer) nanomaterials via the copolymerization of 3‐acrylaminophenylboronic acid (AAPBA) and different hydrophilic monomers . The Fe 3 O 4 @P(AAPBA‐co‐monomer) showed relatively good performance due to the addition of hydrophilic monomer.…”

Section: Nanomaterials In Glycoproteomicsmentioning

confidence: 99%

Nanomaterials in Proteomics

Sun

Shen

et al. 2019

Adv Funct Materials

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For a deep understanding of the human proteome, the first crucial step is always the effective separation of targeted proteins/peptides from complex samples. In mass spectrometry‐based proteomics, nanomaterials have emerged as a highly effective means to separate targeted proteins/peptides and increase their relative abundance, which is beneficial to conduct mass spectrometric analysis. In particular, nanomaterials with different specific functionalities have received great attention in ordinary proteomics, phosphoproteomics, and glycoproteomics, for which the easily recognizable characteristics of these proteomes take the primary responsibility, such as the hydrophobicity, phosphate groups, cis–diol structure, and hydrophilicity. This review mainly focuses on the recent design and preparation of nanomaterials with specific recognition ability, as well as their application in the aforementioned three types of proteomics.

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“…Another alternative route for enzyme‐free glucose sensing is the use of synthetic boronic acid‐based receptors. A phenylboronic acid (PBA) unit reversibly binds to diols of glucose to form five‐ or six‐membered cyclic boronic esters . Boronic acid derivatives have thus been utilized as the biorecognition element in various sensor platforms involving optical, mechanical, or electromagnetic transducers, as an alternative to glucose oxidase.…”

Section: Introductionmentioning

confidence: 99%

Enzyme‐Free Detection of Glucose with a Hybrid Conductive Gel Electrode

Wustoni

Savva

Sun

et al. 2018

Adv Materials Inter

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Current assays for glucose monitoring rely predominantly on glucose oxidation-catalyzing enzymes because of the high specificity of enzyme-substrate interactions. Enzymes are however expensive, suffer from instability during fabrication, operation and storage, and necessitate complex procedures for integration with transducer materials. These challenges, rendering the enzyme-based sensors disadvantageous for routine glucose monitoring, can be overcome by non-enzymatic sensors. Here, for the enzyme-free detection of glucose, an electroactive gel is developed via one-pot polymerization. The functional material is a hybrid of the conducting polymer poly(3,4-ethylenedioxythiophene):polystyrenesulfonate and a polyacrylamide gel functionalized with phenylboronic acid. As an electrode, the gel exhibits a specific current response to glucose within the standard concentration range measured in the complex bloodlike medium. When integrated as the lateral, micrometer-scale gate electrode of an organic electrochemical transistor (OECT), the channel current is proven to be sensitive to the presence of glucose in the measurement solution. The advantage of the OECT based sensor compared to the amperometric electrode is its miniaturized form, amplified input signal as well the elimination of a reference electrode. Adaptable to different geometries, this conducting gel exhibits multifunctionality within its soft, gel-like architecture, that is, mixed ionic and electronic conductivity and glucose specific electrical response. 2 IntroductionGlucose is the primary nutrient of living systems to perform a variety of cellular metabolic processes including cellular respiration and biosynthesis. [1,2] In case of malfunction, cells may end up not taking up glucose sufficiently, which leads to abnormal levels of glucose build up in the bloodstream. The high concentration of glucose in blood is typically associated with the disease, diabetes. Diabetes is a pandemic disorder which was one of the top ten leading causes of death worldwide in 2015 and it is predicted to become the tenth most burdensome disease by 2030. [3][4][5] A good part of diabetes treatment as well as its early diagnosis involve self-monitoring of the amount of glucose in biological fluids. Thus, powerful, low-cost and easy-to-use glucose sensors are on high demand.The inherent specificity and electrochemical reversibility of enzymes poise them as the biorecognition element of choice for a wide range of metabolites. The majority of current glucose sensors are thus electronic and based on the redox enzyme, glucose oxidase that undergoes redox reactions with the analyte, glucose. In these platforms, the enzyme transforms glucose into gluconolactone and hydrogen peroxide (H2O2). H2O2 reacts with a metal electrode in its vicinity, resulting in a change in the flow of electrical current which is directly proportional to concentration of glucose that has reacted with the enzyme. The electrical potential required to oxidize H2O2 however matches that for the oxidation of several ...

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Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides

Cited by 148 publications

References 61 publications

Boronic acid‐functionalized agarose affinity chromatography for isolation of tropomyosin in fishes

Boronic acid‐functionalized agarose affinity chromatography for isolation of tropomyosin in fishes

Nanomaterials in Proteomics

Enzyme‐Free Detection of Glucose with a Hybrid Conductive Gel Electrode

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