Tailoring a multifunctional magnetic cationic metal–organic framework composite for synchronous enrichment of phosphopeptides/glycopeptides

Abstract

Herein, for the first time, a multifunctional magnetic cationic MOF composite (Fe3O4@ILI-01@Ti4+) was successfully prepared for the synchronous enrichment of phosphopeptides/glycopeptides. The as-prepared Fe3O4@ILI-01@Ti4+ bears attractive properties like abundant surface...

Hydrophilic glutathione-modified flower-like hollow covalent organic frameworks for highly efficient capture of N-linked glycopeptides

“…The Au element with an atomic ratio of 3.11% was successfully embedded, which was much higher than those of Au-based materials reported before. 20,22,23,34 As shown in the high-angle annular dark-field scanning TEM (HAADF-STEM) image of the HFH-COFs@Au@GSH (Fig. 1(G)), the elements including C, N, O, Au, and S were simultaneously discovered in the full hollow structure, which affirmed the successful loading of Au NPs and immobilization of GSH.…”

“…3(D)), indicating the excellent enrichment performance for N-glycopeptides which was better than those obtained in the previous reports based on Au NPs and hydrophilic monomers. 16,22,23,[34][35][36] This may be due to the large surface area, the abundance of accessibility sites, and the inherent high chemical stability of the flower-like hollow structure, which enables the loading of large amounts of Au NPs and subsequent hydrophilic GSH. Table S2 (ESI †) lists detailed information about the identified N-glycopeptides.…”

“…On the other hand, a HILIC-based strategy has received increasing popularity in glycoproteomic research, showing favorable reproducibility, easy operation, and unbiased enrichment toward different N -glycopeptides. 13,14 In recent years, a large number of materials involving graphene, 15,16 mesoporous silica, 17,18 polymer 19 magnetic substrates 20,21 and metal-organic frameworks (MOFs) 22,23 have been widely applied as the substrate for N -glycopeptide enrichment based on hydrophilic interaction. However, these HILIC-based materials always suffered from drawbacks such as relatively low surface area, limited affinity sites, great steric hindrance, and low density of hydrophilic functional molecules, which caused limited enrichment efficiency for N -glycopeptides.…”

Superhydrophilic nanocomposite adsorbents modified via nitrogen-rich phosphonate-functionalized ionic liquid linkers: enhanced phosphopeptide enrichment and phosphoproteome analysis of tau phosphorylation in the hippocampal lysate of Alzheimer's transgenic mice

“…18 Very recently, Jia group has reported the first example to synchronously enrich phosphopeptides and glycopeptides using the magnetic composite adsorbent modified by carboxylate-functionalized imidazolium bromide. 19 In this study, we report our results of new strategy to direct materials design, fabrication of nanocomposites based on mesoporous silica coated graphene, and enrichment evaluation with digested standard proteins and applications in real biosamples (Scheme 1). With the combination of polyethyleneimine (PEI) and the phosphonate-functionalized ionic liquid chelator (PFIL), the resulting adsorbent (G@mSiO 2 @PEI-PFIL-Ti 4+ ) showed desirable superhydrophilicity (zero degree), high contents of phosphonate groups and metal ions, which are key features for the enrichment performance of phosphopeptides with superior specificity, ultralow detection limits and the excellent exclusion effect of biomacromolecular interferents.…”

“…18 Very recently, Jia group has reported the first example to synchronously enrich phosphopeptides and glycopeptides using the magnetic composite adsorbent modified by carboxylate-functionalized imidazolium bromide. 19…”