Tailoring Celluclast® Cocktail’s Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass

Karnaouri, Anthi; Μάτσακας, Λεωνίδας; Bühler, Saskja; Muraleedharan, Madhu Nair; Christakopoulos, Paul

doi:10.3390/catal9110897

Cited by 18 publications

(9 citation statements)

References 37 publications

(49 reference statements)

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“…For example, Barbosa et al [10] employed a combination of different endo-glucanases and with lactose and copper as additives, with hydrothermally pretreated sugarcane straw, and obtained almost a 100% of OS selectivity but just a 6.0% of OSY. Karnaouri et al [13] purposed a fine-tuning of the commercially available enzyme mixture Celluclast through pH modification, multi-stage hydrolysis with buffer exchange, and addition of β-glucosidase inhibitor. They achieved a cellobiose-rich product with a high cellobiose to glucose ratio (37.5) and a cellulose conversion to cellobiose of 9.78% by utilizing organosolv-pre-treated birch biomass; they were able to obtained higher conversions (up to 35.2%), although with a much lower cellobiose to glucose ratio (3.3).…”

Section: Multi-stage Enzymatic Hydrolysis Of Pre-treated Bppmentioning

confidence: 99%

“…There is a limited number of studies in the literature addressing cello-OS production from lignocellulosic materials, and these mainly focus on sugarcane straw [10], corncob residue [8], and forest biomass residues [11][12][13]. On the other hand, there are some references on the application of EH to the untreated and pre-treated banana pseudostem material [14][15][16][17][18][19], although these studies aim at the production of fermentable sugars, and no attention was paid to the possibility of obtaining OS, an aspect that is addressed in the present study.…”

Section: Introductionmentioning

confidence: 99%

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Oligosaccharides production by enzymatic hydrolysis of banana pseudostem pulp

Díaz

Ortega

Benítez

et al. 2021

Biomass Conv. Bioref.

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Banana production generates significant amounts of agricultural wastes, being fiber extraction one of the most relevant alternatives for their valorization. This process produces banana’s pseudostem pulp (BPP) as a byproduct, which shows an interesting composition for the biorefinery’s biochemical platform, with high polysaccharides (68%) and low lignin contents. This work deals with the enzymatic hydrolysis (EH) of raw and hydrothermally pre-treated BPP, focusing on the production of oligosaccharides (OS). Raw BPP hydrolysis with cellulase at different dosages rendered only 3.2% OS yields (OSY). Pectinase addition has not affected EH performance. On the other hand, EH of hydrothermally pre-treated BPP at 150 °C and 170 °C (P150 and P170) allowed to increase OSY up to 28% (P150, 1 FPU of cellulase/g dry biomass, 12 h), being 72% of the solubilized sugars in the form of cello-oligosaccharides. This last condition was subjected to a multi-stage EH strategy without improvements in OSY. An endo-glucanase was also tested, but obtained OSY were lower than cellulase results. Finally, obtained OS demonstrated to stimulate the growth of two Lactobacilli strains. The results show that BPP pre-treated under mild operational conditions is a good candidate for cello-oligosaccharides production by EH using 1 FPU/g DB of cellulase with a simple strategy. Graphical abstract

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Section: Multi-stage Enzymatic Hydrolysis Of Pre-treated Bppmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oligosaccharides production by enzymatic hydrolysis of banana pseudostem pulp

Díaz

Ortega

Benítez

et al. 2021

Biomass Conv. Bioref.

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“…To expand the product range beyond bioethanol, cellooligosaccharides (COS), linear oligomers of β-1,4 linked glucose residues, could also potentially be derived from the cellulose of lignocellulosic materials, and these have attracted attention as higher-value products for the pharmaceutical, chemical, food and feed industries ( Ávila et al, 2021). Water soluble COS up to a degree of polymerisation (DP) of 6 glucose units fall into the category of non-digestible oligosaccharides (NDOs) that are fermentable by a number of gut microbiota including various Lactobacillus and Bifidobacterium species (Karnaouri et al, 2019;Jiao et al, 2014;Roberfroid and Slavin, 2000). As such they have been explored as potential prebiotic compounds that can be added to enrich dietary products and animal feed to support gastrointestinal health and function (Otsuka et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…During conventional cellulose hydrolysis with most commercial enzyme preparations, COS only exist as transitory intermediates. Nevertheless, through modification of commercial enzyme cocktails to reduce β-glucosidase activity, including chromatographic fractionation and the addition of inhibitors, as well as splitting the standard singlestep hydrolysis reaction into multiple stages, COS recovery has been demonstrated (Chu et al, 2014;Karnaouri et al, 2019). However, typically these still generate a significant glucose fraction and currently the only COS that can be produced in significant quantities via this method is cellobiose (DP2).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic generation of short chain cello-oligosaccharides from Miscanthus using different pretreatments

Kendrick

Bhatia

Barbosa

et al. 2022

Bioresource Technology

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“…They are obtained from lignocellulosic sources by depolymerization, either by hydrolysis or by oxidative routes. Hydrolysis of residual cellulose can be achieved using endo-and exoglucanases with a reduce activity of β-glucosidases [19] or modified carbon catalysts [20]. Enzyme-driven lytic oxidation is performed with polysaccharide monooxygenases (LPMOs) [21], enzymes that are present nowadays Fermentation 2020, 6, 31 3 of 27in most up-to-date cellulolytic enzyme industrial preparations.…”

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confidence: 99%

Production of Oligosaccharides from Agrofood Wastes

et al. 2020

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The development of biorefinery processes to platform chemicals for most lignocellulosic substrates, results in side processes to intermediates such as oligosaccharides. Agrofood wastes are most amenable to produce such intermediates, in particular, cellooligo-saccharides (COS), pectooligosaccharides (POS), xylooligosaccharides (XOS) and other less abundant oligomers containing mannose, arabinose, galactose and several sugar acids. These compounds show a remarkable bioactivity as prebiotics, elicitors in plants, food complements, healthy coadyuvants in certain therapies and more. They are medium to high added-value compounds with an increasing impact in the pharmaceutical, nutraceutical, cosmetic and food industries. This review is focused on the main production processes: autohydrolysis, acid and basic catalysis and enzymatic saccharification. Autohydrolysis of food residues at 160-190 • C leads to oligomer yields in the 0.06-0.3 g/g dry solid range, while acid hydrolysis of pectin (80-120 • C) or cellulose (45-180 • C) yields up to 0.7 g/g dry polymer. Enzymatic hydrolysis at 40-50 • C of pure polysaccharides results in 0.06-0.35 g/g dry solid (DS), with values in the range 0.08-0.2 g/g DS for original food residues.Fermentation 2020, 6, 31 2 of 27 forestall engineering approaches, to name a few. Lignocellulosic biomass, of any origin is, therefore, a most promising raw material for biorefineries, considering such facilities as integrated refineries turning biomass into fuels, platform chemicals, food, feed and materials using integrated processes with an optimized used of resources [4]. This vision can be extended to resources of aquatic origin (seaweed, seagrass and microalgae) as well as residues from livestock [5,6]. In general, apart from forestall and energy crops biomass, most of it depends on the production of biomass and, in particular, food wastes [7,8]. While more than 100,000 M tons of biomass wastes are yearly produced [7], wastes strictly considered as food wastes (foods not consumed from any part of the food supply chain or any part of the food that is non edible and, therefore, becomes a residue) account for more than 1300 M tons each year [8]. The valorization of biomass wastes into a plethora of useful energy vectors, chemical compounds and ingredients receives a notable amount of interest from all stakeholders, including researchers and entrepreneurs. They are a source to several value-added products, such as monosaccharides (glucose, xylose, mannose, fructose, arabinose and more), oligosaccharides (fructoor FOS, xylo-or XOS, galacto-or GOS, galacturonic-or GALOS, lactosucrose, etc.), biofuels (ethanol, butanol, dimethylether -DME-, biodiesel, hydrogen), bioactive compounds (flavonoids, phenolic acids, terpenes, terpenoids, carotenoids), nanocellulose (bacterial, wood-related), lignin and its derivatives (a source of aromatics from biomass and prospective substitute of the aromatic or BTEX fraction produced in oil refineries) [8]. Oligomers from cellulose, hemicellulose, lignin, pectin and oth...

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Tailoring Celluclast® Cocktail’s Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass

Cited by 18 publications

References 37 publications

Oligosaccharides production by enzymatic hydrolysis of banana pseudostem pulp

Oligosaccharides production by enzymatic hydrolysis of banana pseudostem pulp

Enzymatic generation of short chain cello-oligosaccharides from Miscanthus using different pretreatments

Production of Oligosaccharides from Agrofood Wastes

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