Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Abstract

Carbon dioxide capture, conversion, and utilization with carbonic anhydrase (CA) as a green and sustainable method still faces the challenges of complex and costly purification, relatively low yield, and poor catalytic performances and reusability. Herein, we proposed an all-in-one strategy to solve these problems by self-assembling nanosized CA oligomeric particles (nCAOPs) into micrometer-sized CA supraparticles (mCASPs) with well-designed tags. The preparation of the purified mCASPs was simple and effective by an easy scalable low-speed centrifugation method with 78% activity recovery. The obtained mCASPs with a yield of 870 mg/L was the reported highest yield of CAs. Interestingly, mCASPs could serve as carrier-free immobilized CAs and retain over 90% original activity after 15 reuse cycles. More encouragingly, mCASPs could redissolve and form nCAOPs, which had excellent catalytic performances. The hydrated activity of nCAOPs was 1.05 times that of free CAs. Also, the k cat/K m value was 1.74 times that of free CAs, and the half-life at 40 °C was 3.83 times that of free CAs. Due to the simplicity of purification and immobilization, high yield, and excellent enzymatic properties, mCASPs and nCAOPs are considered novel bioactive materials, which offer the feasibility of CAs for sustainable CO2 capture to achieve the target of carbon neutrality.

Cost-Effective Preparation of Carbonic Anhydrase with Superior Performance for Assisting Amine and Amino Acid Ionic Liquid Blends in CO2 Absorption and Desorption

“…Considering the simple purification process, high purity, and low cost of DvCA8.0-F, it will have great potentials for large-scale preparation. By staining on the micron-scale DvCA8.0-F with crystal violet, rod-shaped and ellipsoidal morphologies (Figure S4d) with sizes of about 1–2 μm were detected under an optical microscope, which was similar to some previous studies. ,− The formation of micron-sized supramolecules may be attributed to the complex internal and external forces, such as the hydrophobic or hydrophilic interactions, as well as electrostatic interactions between DvCA8.0 and ferritin subunits. , Additionally, high protein concentration in the cells also increases the risk of protein–protein interactions and aggregation …”

“…Finally, the DvCA8.0-F with 95% purity (Figure 1b) was obtained with a yield of 800 mg/L. According to cost of the medium 57 based on a laboratory scale, it required a mere ¥22.7 (about $3.1) for the production of 1 g of DvCA8.0-F. Considering the simple purification process, high purity, and low cost of DvCA8.0-F, it will have great potentials for large-scale preparation.…”

“…In our previous study, ferritin was employed as a tag to realize the efficient preparation of bovine CAs (bCAs, α-class, monomeric) and Sulfurihydrogenibium azorense CAs (SazCA, α-class, dimeric). We hope that this method can be applied to more types of CAs, such as β-class CAs, or CAs with more subunits.…”

Templated Droplet Evaporation‐Based Supraparticles in Environmental Applications

“…The development of catalytic SPs is a promising strategy for enhanced CO 2 reduction ability of materials. Liu et al [120] recently developed SPs with tailored properties of self-assembled carbonic anhydrase SPs for CO 2 capture. They developed SPs with simple purification and immobilization method with high yield, and excellent enzymatic properties of mCASPs and nCAOPs for sustainable CO 2 capture.…”

A review of CO2 catalytic regeneration research based on MEA solution

“…by using chemical or physical methods (Li et al, 2016;Fu et al, 2022). It mainly includes adsorption, lowtemperature distillation, membrane, biomimetic, physical, and chemical absorption technology (Davy, 2009;McGurk et al, 2017;Yousef et al, 2017;Luis Míguez et al, 2018;Liu et al, 2022). Among these methods, the chemical absorption is applied widely in industries because of its large absorption capacity, fast absorption rate (Shakerian et al, 2015), ability to handle large amounts of gas, and applicability to low CO 2 partial pressure gases (Muchan et al, 2022).…”