Tailoring the Surface of a Gene Delivery Vector with Carboxymethylated Dextran: A Systematic Analysis

Abstract

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Two Complementary Approaches for the Controlled Release of Biomolecules Immobilized via Coiled-Coil Interactions: Peptide Core Mutations and Multivalent Presentation

“…20-kDa technical grade dextran was first carboxymethylated as previously described . Briefly, dextrans were dissolved in water with NaOH, and the reaction was initiated by adding a solution of monobromoacetic acid (final concentrations: 35 mg/mL dextran, 2.63 M NaOH and 0.95 M monobromoacetic acid).…”

“…The preparations of PEC with various E5 surface densities were quantified by fluorescence readings. The nanoparticles were further evaluated by dynamic light scattering for hydrodynamic diameter and zeta potential measurements on a ZetaSizer Nano ZS from Malvern Instruments (Montreal, QC), as previously described …”

“…20-kDa technical grade dextran was first carboxymethylated as previously described. 30 Briefly, dextrans were dissolved in water with NaOH, and the reaction was initiated by adding a solution of monobromoacetic acid (final concentrations: 35 mg/mL dextran, 2.63 M NaOH and 0.95 M monobromoacetic acid). The reaction was carried out at room temperature under constant stirring, then quenched with HCl (final concentration: 1.95 M) after either 30 or 120 min to obtain CMD20− 30 and CMD20−120, respectively.…”

“…The nanoparticles were further evaluated by dynamic light scattering for hydrodynamic diameter and zeta potential measurements on a ZetaSizer Nano ZS from Malvern Instruments (Montreal, QC), as previously described. 30 SPR-Based Assays. Real-time kinetic studies were performed at 25 °C on a Biacore T100 biosensor, using HBS-EP as running buffer.…”

Adequate Reducing Conditions Enable Conjugation of Oxidized Peptides to Polymers by One-Pot Thiol Click Chemistry

“…Dextran carboxymethylation and characterization were performed as previously described . Briefly, 400 mg of dextran was dissolved in 10 mL of a solution containing 3 M NaOH and 1 M monochloroacetic acid.…”