TAK1 of blunt snout bream promotes NF-κB activation via interaction with TAB1 in response to pathogenic bacteria

Molecular Characterization and Expression Analysis of TAK1, TAB1 and TAB2 of Golden Pompano (Trachinotus ovatus)

“…These domains are conserved in the large yellow croaker, human, emperor penguin and western painted turtle (Figure 2). Similar results were also observed in TAK1s of grass carp [24], Japanese flounder [30], blunt snout bream (Megalobrama amblycephala) [41] and mammals [42,43]. The S_TKc domain plays an important role in the interaction between TAK1 and TAB1 and is essential for the activation of IKK and MAPKs [44,45].…”

“…In mammals, TAK1 and its binding proteins (TAB1, TAB2 and TAB3), which are important signal transduction proteins in MyD88-dependent TLR signaling pathways, can activate the host immune response [61][62][63][64]. In addition, previous studies have shown that TAK1 is involved in the innate immune response and inducing the activity of NF-κB by forming a complex with TAB1 and TAB2 in teleost fishes [22,23,25,41]. In the present study, following LPS stimulation, the expression level of trTAK1 reached its highest level in the HK and spleen at 12 h. In contrast, trTAB1 reached its highest level in the HK and spleen at 12 h and 24 h, respectively, whereas the expression of trTAB2 in the HK and spleen peaked at 24 h. The changes in the expression patterns of these three pompano genes were consistent with those observed in previous studies of other fish.…”