Tamoxifen citrate: a glimmer of hope for silicosis

Yoldaş, Ömer; Karaca, Turgut; Bilgin, Bülent Çağlar; Yılmaz, Ömer Hınç; Şimşek, Gülçin Güler; Alıcı, İbrahim Onur; Uzdogan, Andac; Karaca, Nihal; Akın, Tezcan; Yoldaş, Suna Sadıç; Akbıyık, Filiz

doi:10.1016/j.jss.2014.08.013

Cited by 8 publications

(12 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is an incurable but preventable disease. Pulmonary injury develops as a result of inhalation of crystalline silica nanoparticles and this results with progressive lung fibrosis (17). Oxidative stress is thought to be one of the main mechanisms in the development of fibrosis induced by silica.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Dynamic Disulphide/Thiol Homeostasis in Silica Exposed Workers

et al. 2017

View full text Add to dashboard Cite

Background:Oxidative stress is implicated as one of the main molecular mechanism underlying silicosis.Aims:In this study, our aim was to asses the redox status in occupationally silica-exposed workers, by evaluating the dynamic thiol-disulphide homeostasis.Study Design:Case-control study.Methods:Thirty-six male workers occupationally exposed to silica particles and 30 healthy volunteers, working as office workers were included to the study. Posteroanterior chest radiographs and pulmonary function tests of both groups were evaluated. Also serum thiol disulphide levels were measured using the spectrophotometric method described by Erel and Neşelioğlu.Results:Among the 36 workers that underwent pulmonary function tests 6 (17%) had obstructive, 7 (19%) had restrictive, 6 (17%) had obstructive and restrictive signs whereas 17 (47%) had no signs. The mean PFTs results of silica-exposed workers were significantly lower than control subjects. The serum disulphide levels of silica-exposed workers were significantly higher than control subjects (23.84±5.89 μmol/L and 21.18±3.44 μmol/L, respectively p=0.02).Conclusion:The serum disulphide levels, a biomarker of oxidative stress, are found to be higher in silica-exposed workers.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of Dynamic Disulphide/Thiol Homeostasis in Silica Exposed Workers

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Four-micrometer-thick radial sections (3 or 4 slices) were taken from the paraffin embedding which contains the region between primary and defective tissue zones with a microtome, and sections were stained with hematoxylin and eosin (H&E). Inflammation, vascular proliferation, and fibrosis were evaluated considering the prevalence and severity of inflammatory cells, new vessels, and fibroblasts in tissue specimens in microscopic evaluation of 40x high power field (HPF) [ 26 ]. Grade 0 indicated that there was no inflammation, vascular proliferation, or fibrosis.…”

Section: Methodsmentioning

confidence: 99%

“…In order to analyse the antibody expressions, immunohistochemical staining patterns were scored with the staining intensity and morphology, a combination of qualitative and quantitative information, by the same examiner [ 26 ]. Grades 0, 1, and 2 indicated no staining, minimal staining, and severe staining, respectively.…”

Section: Methodsmentioning

confidence: 99%

A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting

Can¹,

Çakmak

Çan

et al. 2016

Journal of Ophthalmology

View full text Add to dashboard Cite

Purpose. To investigate the effect of platelet-rich fibrin (PRF) membrane on wound healing. Methods. Twenty-four right eyes of 24 New Zealand rabbits equally divided into 2 groups for the study design. After the creation of 5 × 5 mm conjunctival damage, it was secured with PRF membrane, which was generated from the rabbit's whole blood samples in PRF membrane group, whereas damage was left unsutured in the control group. Three animals were sacrificed in each group on the 1st, 3rd, 7th, and 28th postoperative days. Immunohistochemical (IHC) stainings and biomicroscopic evaluation were performed and compared between groups. Results. PRF membrane generated significant expressions of vascular endothelial growth factor (VEGF), transforming growth factor-beta (TGF-β), and platelet-derived growth factor (PDGF) in the early postoperative period. However, the IHC evaluation allowed showing the excessive staining at day 28, in control group. Biomicroscopic evaluation revealed complete epithelialization in PRF membrane group, but none of the cases showed complete healing in the control group. Conclusions. This experimental study showed us the beneficial effects of the PRF membrane on conjunctival healing. Besides its chemical effects, it provides mechanical support as a scaffold for the migrating cells that are important for ocular surface regeneration. These overall results encourage us to apply autologous PRF membrane as a growth factor-enriched endogenous scaffold for ocular surface reconstruction.

show abstract

“…Tamoxifen has been shown to inhibit renal fibrosis (Lee et al, 2009;Delle et al, 2012), retroperitoneal fibrosis (Ozener et al, 1997;Huang et al, 2011;van Bommel et al, 2006), hypertrophic scarring (Mousavi et al, 2010) and Dupuytren disease (Degreef et al, 2014), as well as silicainduced lung fibrosis (Yoldas et al, 2015). In these studies, the effect of tamoxifen has been linked to a downregulation in the expression of TGF-b and other growth factors in affected tissues (Mikulec et al, 2001;Delle et al, 2012;Yoldas et al, 2015). Although we did not explore changes in the expression of autocrine growth factors in our experiments, we clearly demonstrate that tamoxifen prevents TGF-b-induced myofibroblast differentiation in cultured fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

“…Further, it would be worth exploring the potential benefits of tamoxifen in other cancers where activated fibroblasts are prominent. As mentioned above, many studies already point to tamoxifen having such an effect on activated fibroblasts in vivo in models of fibrosis (Degreef et al, 2014;Kim et al, 2014;Yoldas et al, 2015).…”

mentioning

confidence: 97%

Tamoxifen Inhibits TGF‐β‐Mediated Activation of Myofibroblasts by Blocking Non‐Smad Signaling Through ERK1/2

Carthy

Sundqvist

Heldin

et al. 2015

Journal Cellular Physiology

View full text Add to dashboard Cite

Transforming growth factor-β (TGF-β) is a multifunctional cytokine which stimulates the differentiation of fibroblasts into myofibroblasts. Myofibroblasts are critical for normal wound healing, but also accumulate pathologically in a number of chronic inflammatory conditions where they are key contributors to aberrant tissue remodeling and fibrosis, and in cancer stroma. In the current study, we identified a role for tamoxifen as a potent inhibitor of the TGF-β-mediated activation of primary human skin and breast fibroblasts. Our data indicate that tamoxifen does not interfere with canonical Smad signaling downstream of TGF-β but rather blocks non-Smad signaling through ERK1/2 MAP-kinase and the AP-1 transcription factor FRA2. We further demonstrate by siRNA-mediated knockdown that FRA2 is critical for the induced expression of myogenic proteins in response to TGF-β. Functionally, TGF-β-stimulated fibroblast-mediated contraction of collagen gels was impaired in the presence of tamoxifen. Altogether, these data demonstrate that tamoxifen prevents myofibroblast differentiation and, therefore, may provide therapeutic benefits to patients suffering from chronic inflammatory conditions or cancer.

show abstract

Tamoxifen citrate: a glimmer of hope for silicosis

Cited by 8 publications

References 16 publications

Evaluation of Dynamic Disulphide/Thiol Homeostasis in Silica Exposed Workers

Evaluation of Dynamic Disulphide/Thiol Homeostasis in Silica Exposed Workers

A Novel Technique for Conjunctivoplasty in a Rabbit Model: Platelet-Rich Fibrin Membrane Grafting

Tamoxifen Inhibits TGF‐β‐Mediated Activation of Myofibroblasts by Blocking Non‐Smad Signaling Through ERK1/2

Contact Info

Product

Resources

About