Tandem mass tagging combined with liquid chromatography–tandem mass spectrometry technique to detect protein markers in gastroesophageal junction adenocarcinoma

Zheng, Xiuli; Bi, Yanna; Yang, None Tianshuo; Zhao, Lianmei; Wu, Ming-li; Er, Limian; Liu, Yao; Li, Shengmian

doi:10.1002/rcm.9355

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…The peptide mixture obtained in the previous step was subjected to LC–MS/MS analysis as described in the previous report 23 …”

Section: Methodsmentioning

confidence: 99%

Lung proteomics combined with metabolomics reveals molecular characteristics of inflammation‐related lung tumorigenesis induced by B(a)P and LPS

Miao

et al. 2023

Environmental Toxicology

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Inflammatory microenvironment may take a promoting role in lung tumorigenesis. However, the molecular characteristics underlying inflammation‐related lung cancer remains unknown. In this work, the inflammation‐related lung tumorigenesis mouse model was established by treated with B(a)P (1 mg/mouse, once a week for 4 weeks), followed by LPS (2.5 μg/mouse, once every 3 weeks for five times), the mice were sacrificed 30 weeks after exposure. TMT‐labeled quantitative proteomics and untargeted metabolomics were used to interrogate differentially expressed proteins and metabolites in different mouse cancer tissues, followed by integrated crosstalk between proteomics and metabolomics through Spearman's correlation analysis. The result showed that compared with the control group, 103 proteins and 37 metabolites in B(a)P/LPS group were identified as significantly altered. By searching KEGG pathway database, proteomics pathways such as Leishmaniasis, Asthma and Intestinal immune network for IgA production, metabolomics pathways such as Vascular smooth muscle contraction, Linoleic acid metabolism and cGMP‐PKG signaling pathway were enriched. A total of 22 pathways were enriched after conjoint analysis of the proteomic and metabolomics, and purine metabolism pathway, the unique metabolism‐related pathway, which included significantly altered protein (adenylate cyclase 4, ADCY4) and metabolites (L‐Glutamine, guanosine monophosphate (GMP), adenosine and guanosine) was found. Results suggested purine metabolism may contribute to the inflammation‐related lung tumorigenesis, which may provide novel clues for the therapeutic strategies of inflammation‐related lung cancer.

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“…The peptide mixture obtained in the previous step was subjected to LC–MS/MS analysis as described in the previous report 23 …”

Section: Methodsmentioning

confidence: 99%

Lung proteomics combined with metabolomics reveals molecular characteristics of inflammation‐related lung tumorigenesis induced by B(a)P and LPS

Miao

et al. 2023

Environmental Toxicology

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New insights into pathogenesis of congenital pseudarthrosis of tibia in children using periosteum proteomics analysis

Liu

Qin

Zheng

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale: The exact etiology and pathogenesis of congenital pseudarthrosis of tibia (CPT) are not clear. Quantitative proteomics analysis plays a vital role in disease pathology research. Tandem mass tag (TMT)-based proteomics techniques were employed to identify and analyze the differentially expressed proteins (DEP) in the tibia periosteum tissues of CPT patients. Methods: The samples were divided into three groups: CPT with NF1 group, CPT without NF1 group (non-NF1-CPT), and control group (patients with open tibial fracture). A fold change ≥1.5 or ≤0.66 and P-value <0.05 were used as the thresholds to screen DEPs. Subsequently, bioinformatics resources such as online tools DAVID and String were used to generate gene ontology (GO) annotation, KEGG pathways enrichment, and protein-protein interaction (PPI) network for these DEPs. Results:The results show that a total of 347 proteins were differentially expressed in NF1-CPT groups, 212 of which were upregulated and 135 were downregulated.There were more DEPs in non-NF1-CPT groups; we identified 467 DEPs, including 281 upregulated and 186 downregulated. Among them, NF1-CPT groups and non-NF1-CPT groups shared 231 DEPs, and the remaining 230 DEPs showed the same expression trend in the two disease groups, with 117 upregulated and 113 downregulated. In particular, 116 proteins were altered only in NF1-CPT groups (94 were upregulated and 22 were downregulated), whereas 236 proteins were altered only in non-NF1-CPT groups (164 were upregulated and 72 were downregulated). Finally, compared with non-NF1-CPT groups, 47 proteins changed 1.5-fold and P-value < 0.05 in NF1-CPT groups.Conclusions: To sum up, we found that common DEPS in periosteum of NF1-CPT and non-NF1-CPT groups are mainly involved in cell matrix assembly, cell adhesion, AKT-PI3K signal pathway activation, and vascular agglutination, which indicate that these are the pathological characteristics of CPT. The osteogenic ability is weak, the osteoclastic ability is strong, the vascular lumen is narrow, the invasive growth and the proliferation of fibroblasts are enhanced in CPT patients.

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Tandem mass spectrometry tag proteomic analysis of milk and colostrum of Guanzhong dairy goat

Lu,

Mengqi,

Yuxin

et al. 2024

International Dairy Journal

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Tandem mass tagging combined with liquid chromatography–tandem mass spectrometry technique to detect protein markers in gastroesophageal junction adenocarcinoma

Cited by 3 publications

References 42 publications

Lung proteomics combined with metabolomics reveals molecular characteristics of inflammation‐related lung tumorigenesis induced by B(a)P and LPS

Lung proteomics combined with metabolomics reveals molecular characteristics of inflammation‐related lung tumorigenesis induced by B(a)P and LPS

New insights into pathogenesis of congenital pseudarthrosis of tibia in children using periosteum proteomics analysis

Tandem mass spectrometry tag proteomic analysis of milk and colostrum of Guanzhong dairy goat

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