Tanning of the tarsal and mandibular cuticle in adult Anax imperator (Insecta: Odonata) during the emergence sequence

Abstract

The arthropod cuticle offers strength, protection, and lightweight. Due to its limit in expandability, arthropods have to moult periodically to grow. While moulting is beneficial in terms of parasite or toxin control, growth and adaptation to environmental conditions, it costs energy and leaves the soft animal's body vulnerable to injuries and desiccation directly after ecdysis. To investigate the temporal change in sclerotization and pigmentation during and after ecdysis, we combined macrophotography, confocal laser scanning microscopy, scanning electron microscopy and histological sectioning. We analysed the tarsal and mandibular cuticle of the blue emperor dragonfly to compare the progress of tanning for structures that are functionally involved during emergence (tarsus/tarsal claws) with structures whose functionality is required much later (mandibles). Our results show that: (i) the tanning of the tarsal and mandibular cuticle increases during emergence; (ii) the tarsal cuticle tans faster than the mandibular cuticle; (iii) the mandibles tan faster on the aboral than on the oral side; and (iv) both the exo- and the endocuticle are tanned. The change in the cuticle composition of the tarsal and mandibular cuticle reflects the demand for higher mechanical stability of these body parts when holding on to the substrate during emergence and during first walking or hunting attempts.

Editorial: composite materials in biological and bioinspired systems

“…The tanned arthropod cuticle provides essential strength, protection, and lightweight properties, yet its limited expandability necessitates periodic moulting for growth. Investigating the temporal changes in sclerotization and pigmentation during and after moulting, the study by Preuss et al [ 17 ] on the tarsal and mandibular cuticle of the dragonfly Anax imperator reveals differing tanning rates and patterns. Specifically, the faster tanning of the tarsal cuticle compared to the mandibular cuticle suggests a demand for increased mechanical stability in structures involved in emergence and initial locomotion or hunting attempts.…”