TaNTF2 , a contributor for wheat resistance to the stripe rust pathogen

Zhang, Qiong; Wang, Bing; Wei, Jinping; Wang, Xiaojie; Han, Qingmei; Kang, Zhensheng

doi:10.1016/j.plaphy.2017.12.020

Cited by 14 publications

(10 citation statements)

References 36 publications

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“…The up-representation of NTF2 was observed previously in the compatible interaction of Verticillium dahliae with tomato (Hu et al, 2019). Furthermore, the silencing of TaNTF2 reduced the resistance of wheat to different avirulent isolates of the Puccinia striiformis, which speculated that NTF2 acted as a critical regulator in the Ran-mediated signal transduction in the plant immune system (Zhang et al, 2018b).…”

Section: Translation and Transport-related Proteinssupporting

confidence: 58%

Proteomic Analysis of Fusarium oxysporum-Induced Mechanism in Grafted Watermelon Seedlings

Zhang

Ren

et al. 2021

Front. Plant Sci.

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Grafting can improve the resistance of watermelon to soil-borne diseases. However, the molecular mechanism of defense response is not completely understood. Herein, we used a proteomic approach to investigate the molecular basis involved in grafted watermelon leaf defense against Fusarium oxysporum f.sp. niveum (FON) infection. The bottle gourd rootstock-grafted (RG) watermelon seedlings were highly resistant to FON compared with self-grafted (SG) watermelon plants, with a disease incidence of 3.4 and 89%, respectively. Meanwhile, grafting significantly induced the activity of pathogenesis-related proteases under FON challenge. Proteins extracted from leaves of RG and SG under FON inoculation were analyzed using two-dimensional gel electrophoresis. Thirty-nine differentially accumulated proteins (DAPs) were identified and classified into 10 functional groups. Accordingly, protein biosynthetic and stress- and defense-related proteins play crucial roles in the enhancement of disease resistance of RG watermelon seedlings, compared with that of SG watermelon seedlings. Proteins involved in signal transduction positively regulated the defense process. Carbohydrate and energy metabolism and photosystem contributed to energy production in RG watermelon seedlings under FON infection. The disease resistance of RG watermelon seedlings may also be related to the improved scavenging capacity of reactive oxygen species (ROS). The expression profile of 10 randomly selected proteins was measured using quantitative real-time PCR, among which, 7 was consistent with the results of the proteomic analysis. The functional implications of these proteins in regulating grafted watermelon response against F. oxysporum are discussed.

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Section: Translation and Transport-related Proteinssupporting

confidence: 58%

Proteomic Analysis of Fusarium oxysporum-Induced Mechanism in Grafted Watermelon Seedlings

Zhang

Ren

et al. 2021

Front. Plant Sci.

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“…striiformis f. sp. tritici 60 . Since MLP124017 has been shown to interact with TOPLESS and TOPLESS-related proteins 22 , it is tempting to speculate that the cavity formed by the β-sheet could be involved in the association with these plant partners.…”

Section: Discussionmentioning

confidence: 99%

Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds

Guillen

Lorrain

Tsan

et al. 2019

Sci Rep

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Rust fungi are plant pathogens that secrete an arsenal of effector proteins interfering with plant functions and promoting parasitic infection. Effectors are often species-specific, evolve rapidly, and display low sequence similarities with known proteins. How rust fungal effectors function in host cells remains elusive, and biochemical and structural approaches have been scarcely used to tackle this question. In this study, we produced recombinant proteins of eleven candidate effectors of the leaf rust fungus Melampsora larici-populina in Escherichia coli. We successfully purified and solved the three-dimensional structure of two proteins, MLP124266 and MLP124017, using NMR spectroscopy. Although both MLP124266 and MLP124017 show no sequence similarity with known proteins, they exhibit structural similarities to knottins, which are disulfide-rich small proteins characterized by intricate disulfide bridges, and to nuclear transport factor 2-like proteins, which are molecular containers involved in a wide range of functions, respectively. Interestingly, such structural folds have not been reported so far in pathogen effectors, indicating that MLP124266 and MLP124017 may bear novel functions related to pathogenicity. Our findings show that sequence-unrelated effectors can adopt folds similar to known proteins, and encourage the use of biochemical and structural approaches to functionally characterize effector candidates.

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“…, 2004; Zhang et al. , 2018). There are three GmRan proteins encoded by Glyma.06G070500, Glyma.06G070600 and Glyma.06G070700 in soybean.…”

Section: Resultsmentioning

confidence: 99%

“…, 2004; Zhang et al. , 2018). Soybean GmNTF2B‐1 shares strong sequence similarity with animal GmNTF2B‐1 and exhibits comparable intracellular presence (Zang et al.…”

Section: Discussionmentioning

confidence: 99%

Nuclear transport factor GmNTF2B‐1 enhances soybean drought tolerance by interacting with oxidoreductase GmOXR17 to reduce reactive oxygen species content

Chen

Tang

et al. 2021

The Plant Journal

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SUMMARY Drought is a critical abiotic stressor that modulates soybean yield. Drought stress drastically enhances reactive oxygen species (ROS) formation, and maintaining ROS content above a cytostatic level but below a cytotoxic level is essential for normal biology processes in plants. At present, most of the known ROS‐scavenging systems are in the cytoplasm, and the mechanism of ROS regulation in the nucleus remains unclear. GmNTF2B‐1 is a member of the IV subgroup in the nucleus transporter family. Its expression is localized to the roots and is stimulated by drought stress. In this study, the overexpression of GmNTF2B‐1 was found to improve the drought tolerance of transgenic soybean by influencing the ROS content in plants. An oxidoreductase, GmOXR17, was identified to interact with GmNTF2B‐1 in the nucleus through the yeast two‐hybrid, coimmunoprecipitation and bimolecular fluorescence complementation assays. The drought tolerance of GmOXR17 transgenic soybean was similar to that of GmNTF2B‐1. GmNTF2B‐1 was expressed in both cytoplasm and nucleus, and GmOXR17 transferred from the cytoplasm to the nucleus under drought stress. The overexpression of GmNTF2B‐1 enhanced the nuclear entry of GmOXR17, and the overexpression of GmNTF2B‐1 or GmOXR17 could decrease the H2O2 content and oxidation level in the nucleus. In conclusion, the interaction between GmNTF2B‐1 and GmOXR17 may enhance the nuclear entry of GmOXR17, thereby enhancing nuclear ROS scavenging to improve the drought resistance of soybean.

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TaNTF2 , a contributor for wheat resistance to the stripe rust pathogen

Cited by 14 publications

References 36 publications

Proteomic Analysis of Fusarium oxysporum-Induced Mechanism in Grafted Watermelon Seedlings

Proteomic Analysis of Fusarium oxysporum-Induced Mechanism in Grafted Watermelon Seedlings

Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds

Nuclear transport factor GmNTF2B‐1 enhances soybean drought tolerance by interacting with oxidoreductase GmOXR17 to reduce reactive oxygen species content

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