Tanycytes in the medial habenular nucleus of the rat

Cupedo, Rnj; Deweerd, H

doi:10.1007/bf00318938

Cited by 10 publications

(3 citation statements)

References 21 publications

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“…The location of their cell body, their morphology, the connection of their single basal process to blood vessels, and our finding of ADAM21 in hypothalamic tanycytes raise the possibility that the subependymal cells are related to tanycytes (Bruni, 1998). Although tanycytes have been described and studied predominantly around the third ventricle, they have also been found around the cerebral aqueduct, the fourth ventricle, and the central canal in the spinal cord and in the medial habenular nucleus Felten et al, 1981;Cupedo and de Weerd, 1985;Rafols and Goshgarian, 1985). A few tanycytes have also been described in the adult mouse SVZ (Doetsch et al, 1997).…”

Section: Adam21-positive Processes May Direct Adult Neuroblast Migrationmentioning

confidence: 71%

A disintegrin and metalloprotease 21 (ADAM21) is associated with neurogenesis and axonal growth in developing and adult rodent CNS

Yang

Baker

Hagg

2005

J of Comparative Neurology

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We have reported that alpha6beta1 integrin regulates the directed migration of neuroblasts from the adult rodent subventricular zone (SVZ) through the rostral migratory stream (RMS). ADAM (a disintegrin and metalloprotease) proteins bind integrins. Here, we show that ADAM21, but not ADAM2, -3, -9, -10, -12, -15, or -17, is expressed in adult rats and mice by ependyma and SVZ cells with long basal processes, and in radial glia at early postnatal times. ADAM21-positive processes projected into the RMS, contacted blood vessels, and were present within the RMS intermingled with neuroblasts up to where neuroblasts start their radial migration and differentiation in the olfactory bulb. Tissue inhibitors of metalloproteases (TIMPs) 1, 2, and 3 are present in the ependymal layer but not in the SVZ and RMS. Thus, ADAM21 could regulate neurogenesis and guide neuroblast migration through cleavage-dependent activation of proteins and integrin binding. ADAM21 is also present in growing axonal tracts during postnatal development and in growing primary olfactory axons in adults. In the olfactory nerve layer, ADAM21 often, but not always, colocalizes with OMP, a marker of mature olfactory neurons, but is not colocalized with the immature marker betaIII-tubulin. This suggests that ADAM21 is involved in the final axonal outgrowth phase and/or synapse formation. TIMP3 is present in periglomerular neurons, where it could restrict ADAM21-mediated axonal growth to the glomeruli. ADAM21's unique disintegrin and metalloprotease sequences and its restricted expression suggest that it might be a good target for influencing neurogenesis and neuronal plasticity.

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Section: Adam21-positive Processes May Direct Adult Neuroblast Migrationmentioning

confidence: 71%

A disintegrin and metalloprotease 21 (ADAM21) is associated with neurogenesis and axonal growth in developing and adult rodent CNS

Yang

Baker

Hagg

2005

J of Comparative Neurology

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“…In the MHb, very weakly rhythmic temporal expression of PER2::LUC bioluminescence was observed in 5 of 11 cultures, and appeared linked to the waves of expression radiating up the ependymal cell layer (period 24±0.9 h; Movie 1, Figs. 3 and 4), and hence may reflect expression in ependymal tanycytes projecting into this structure (Cupedo and de Weerd, 1985). Forskolin treatment restarted damped rhythms in the ependymal cell layer to a much greater extent than in the LHb (Fig.…”

Section: Resultsmentioning

confidence: 99%

Circadian oscillators in the epithalamus

Guilding¹,

Hughes²,

Piggins³

2010

Neuroscience

107

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The habenula complex is implicated in a range of cognitive, emotional and reproductive behaviors, and recently this epithalamic structure was suggested to be a component of the brain's circadian system. Circadian timekeeping is driven in cells by the cyclical activity of core clock genes and proteins such as per2/PER2. There are currently no reports of rhythmic clock gene/protein expression in the habenula and therefore the question of whether this structure has an intrinsic molecular clock remains unresolved. Here, using videomicroscopy imaging and photon-counting of a PER2::luciferase (LUC) fusion protein together with multiunit electrophysiological recordings, we tested the endogenous circadian properties of the mouse habenula in vitro. We show that a circadian oscillator is localized primarily to the medial portion of the lateral habenula. Rhythms in PER2:: LUC bioluminescence here are visualized in single cells and oscillations continue in the presence of the sodium channel blocker, tetrodotoxin, indicating that individual cells have intrinsic timekeeping properties. Ependymal cells lining the dorsal third ventricle also express circadian oscillations of PER2. These findings establish that neurons and non-neuronal cells in the epithalamus express rhythms in cellular and molecular activities, indicating a role for circadian oscillators in the temporal regulation of habenula controlled processes and behavior.

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“…Cilia on MHb cells are thought to detect regulatory factors (growth factors, hormones, etc.) and xenobiotic agents contained in the cerebrospinal fluid (CSF) of the third ventricle (59,87). Thus, it is an interesting possibility that HHIP/PTCH-1 signaling in primary cilia regulates the detection by MHb neurons of nicotine contained in CSF and to engage nicotine avoidance behaviors.…”

Section: Discussionmentioning

confidence: 99%

Hedgehog-interacting protein acts in the habenula to regulate nicotine intake

Caligiuri

Howe

Wills

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Hedgehog-interacting protein (HHIP) sequesters Hedgehog ligands to repress Smoothened (SMO)-mediated recruitment of the GLI family of transcription factors. Allelic variation in HHIP confers risk of chronic obstructive pulmonary disease and other smoking-related lung diseases, but underlying mechanisms are unclear. Using single-cell and cell-type-specific translational profiling, we show that HHIP expression is highly enriched in medial habenula (MHb) neurons, particularly MHb cholinergic neurons that regulate aversive behavioral responses to nicotine. HHIP deficiency dysregulated the expression of genes involved in cholinergic signaling in the MHb and disrupted the function of nicotinic acetylcholine receptors (nAChRs) through a PTCH-1/cholesterol-dependent mechanism. Further, CRISPR/Cas9-mediated genomic cleavage of the Hhip gene in MHb neurons enhanced the motivational properties of nicotine in mice. These findings suggest that HHIP influences vulnerability to smoking-related lung diseases in part by regulating the actions of nicotine on habenular aversion circuits.

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Tanycytes in the medial habenular nucleus of the rat

Cited by 10 publications

References 21 publications

A disintegrin and metalloprotease 21 (ADAM21) is associated with neurogenesis and axonal growth in developing and adult rodent CNS

A disintegrin and metalloprotease 21 (ADAM21) is associated with neurogenesis and axonal growth in developing and adult rodent CNS

Circadian oscillators in the epithalamus

Hedgehog-interacting protein acts in the habenula to regulate nicotine intake

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