Tao‐Hong‐Si‐Wu decoction reduces ischemia reperfusion rat myoblast cells calcium overloading and inflammation through the Wnt/IP3R/CAMKII pathway

Zhu, Fu-Ping; Li, Wuping; Linhua, Wang; Cheng-xi, Pan; Fuqiang, Zhou; Zhang, Yi; Wang, Aijun

doi:10.1002/jcb.28582

Cited by 15 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chemical ingredients of six herbs, including Paeoniae Radix Alba (Baishao in Chinese), Rehmanniae Radix Praeparata (Shudihuang in Chinese), Angelicae Sinensis Radi x (Danggui in Chinese), Chuanxiong Rhizoma (Chuanxiong in Chinese), Persicae Semen (Taoren in Chinese), and Carthami Flos (Honghua in Chinese) in TSD prescription (the contents of herbs = 9 g: 10 g: 8 g: 6 g: 15 g: 15 g, sequentially), were retrieved from the TCMSP database [ 15 ]. The parameters of oral bioavailability (OB value) ≥ 30% and drug-like property (DL value) ≥ 0.18 were considered as herb screening and evaluation conditions for the ingredients based on recommendations from the TCMSP database and previous research [ 14 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

Analysis of Potential Mechanism of Herbal Formula Taohong Siwu Decoction against Vascular Dementia Based on Network Pharmacology and Molecular Docking

et al. 2023

BioMed Research International

View full text Add to dashboard Cite

Vascular dementia (VaD) is the second most prevalent dementia, which is attributable to neurovascular dysfunction. Currently, no approved pharmaceuticals are available. Taohong Siwu decoction (TSD) is a traditional Chinese medicine prescription with powerful antiapoptosis and anti-inflammatory properties. In this study, a network pharmacology approach together with molecular docking validation was used to explore the probable mechanism of action of TSD against VaD. A total of 44 active components, 202 potential targets of components, and 3,613 VaD-related targets including 161 intersecting were obtained. The potential chemical components including kaempferol, baicalein, beta-carotene, luteolin, quercetin, and beta-sitosterol involved in the inflammatory response, oxidative stress, and apoptosis might have potential therapeutic effects on the treatment of VaD. The potential core targets including AKT1, CASP3, IL1β, JUN, and TP53 associated with cell apoptosis and inflammatory might account for the essential therapeutic effects of TSD in VaD. The results indicated that TSD protected against VaD through multicomponent and multitarget modes. Though the detailed mechanism of action of various active ingredients needs to be further illustrated, TSD still showed a promising therapeutic agent for VaD due to its biological activity.

show abstract

Section: Methodsmentioning

confidence: 99%

Analysis of Potential Mechanism of Herbal Formula Taohong Siwu Decoction against Vascular Dementia Based on Network Pharmacology and Molecular Docking

et al. 2023

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Taohong Siwu decoction (TSD) is a formula consisting of six traditional CHMs of Semen Persicae , Flos Carthami , Angelica Sinensis , Radix Paeoniae Alba , Rhizoma Chuanxiong , and Radix Rehmanniae Praeparata [ 10 ]. TSD was demonstrated to maintain the effects of blood activation [ 10 ], pain relief, and anti-inflammation [ 11 ]. It is often utilized in gynecological and cardiovascular diseases [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Taohong Siwu Decoction Promotes Osteo-Angiogenesis in Fractures by Regulating the HIF-1α Signaling Pathway

Tang

Yin

Guo

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Background. Vascular damage is a major consequence of bone fracture. Taohong Siwu decoction (TSD) can raise the expression of vascular endothelial growth factor (VEGF) in fracture healing. However, its molecular mechanism in promoting angiogenesis is still unknown. The aim of this study was to investigate the potential mechanisms of TSD in the regulation of osteo-angiogenesis in fracture healing. Methods. A rat tibial fracture model was established. After low- (4.5 g·kg−1), medium- (9 g·kg−1), and high-dose TSD (18 g·kg−1) and panax notoginsenoside (25 mg kg−1) treatment, hematoxylin-eosin staining was employed to visualize pathological changes in bone tissues. The levels of cytokines (interleukin (IL)-2, tumor necrosis factor-α (TNF-α), IL-6, and IL-1β), thromboxane B2 (TXB2), and 6 ketone prostaglandin F1α (6-Keto-PGF1α) were quantified by enzyme-linked immunosorbent assay (ELISA). Immunofluorescence was used to identify the rat aortic endothelial cells (RAECs). Control serum, 10% TSD-containing serum, and 10% TSD-containing serum combined with hypoxia-inducible factor-1α (HIF-1α) inhibitor were used to treat the RAECs and rat osteoblasts. Transwell migration assay was utilized to examine the migration of the RAECs. The Matrigel tubulogenesis assay was used for the assessment of angiogenesis. The expression of angiogenesis- (von Hippel-Lindau tumor suppressor (VHL), HIF-1α, VEGF, angiopoietin-2 (Ang-2), and pVHL) and osteogenesis-related (alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and osteopontin-1 (OPN-1)) protein and gene was detected by western blot and quantitative real-time PCR (qRT-PCR). Results. Compared with the model group, TSD increased the trabecular bone areas, numbers, and thicknesses in fractured rats. In the plasma, the levels of cytokines and TXB2 in the middle- and high-dose TSD group were significantly lower than those in the model group ( P < 0.01 ). The 6-keto-PGF1α content was increased by middle- and high-dose TSD intervention ( P < 0.01 ). Compared to the control serum group, the angiogenesis and migration of the RAECs were enhanced in the TSD group ( P < 0.001 ). The expression of HIF-1α, VEGF, and Ang-2 in the TSD group upregulated significantly ( P < 0.001 ). VHL and pVHL were inhibited under TSD-containing serum treatment ( P < 0.001 ). ALP, Runx2, and OPN-1 were increased obviously in the TSD group ( P < 0.001 ). Nevertheless, the HIF-1α inhibitor reversed these changes ( P < 0.001 ). Conclusion. TSD promotes angiogenesis and osteogenesis by regulating the HIF-1α signaling pathway. Meanwhile, it can effectively reduce the risk of inflammation and improve blood circulation.

show abstract

“…THSWD has also been shown to reduce calcium overload and the inflammatory response of myoblast cells in I/R rats, thereby improving myocardial injury caused by I/R (Fuping et al. 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Modified Taohong Siwu decoction improves cardiac function after myocardial ischaemia and reperfusion in rats by promoting endogenous stem cell mobilization and regulating metabolites

Meng

Xiao

et al. 2022

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Taohong Siwu decoction (THSWD) has been shown to promote heart repair in myocardial infarction. Objective To determine the effects of modified THSWD (THSWD plus four ingredients) on myocardial ischaemia and reperfusion (I/R) injury. Materials and methods Sixty Sprague-Dawley rats were randomly divided into the I/R group and three different modified THSWD dose groups (gavage administration, 1.215, 2.43, and 4.86 g, respectively). 2,3,5-Triphenyltetrazolium chloride and Evans blue staining were used to detect the infarct area at 24 h after treatment. The serum biochemical indexes and cell apoptosis were examined to determine myocardial injury. The number of endogenous stem cells, expression of stromal dell derived factor-1 (SDF-1) and stem cell factor (SCF), and cardiac function were measured at 4 weeks. The serum was collected for metabolomic analysis. Results The high-dose modified THSWD group presented a reduced infarction area (decreased by 21.3%), decreased levels of lactate dehydrogenase and creatinine kinase, attenuated cell apoptosis, and enhanced superoxide dismutase activity in early stage I/R compared with other groups. The serum SCF and SDF-1 levels were higher in the high-dose group than in the I/R group. At 4 weeks, the infarct size and collagen content were the lowest, and the ejection fraction and fractional shortening values were the highest in the high-dose group. Moreover, high-dose modified THSWD affected the metabolism of phosphonate and phosphonate, taurine, and hypotaurine. Conclusions Endogenous stem cell mobilization and metabolic regulation were related to the cardioprotection of modified THSWD. We provided a new strategy and direction for the treatment of cardiovascular diseases with traditional Chinese medicine.

show abstract

Tao‐Hong‐Si‐Wu decoction reduces ischemia reperfusion rat myoblast cells calcium overloading and inflammation through the Wnt/IP3R/CAMKII pathway

Cited by 15 publications

References 29 publications

Analysis of Potential Mechanism of Herbal Formula Taohong Siwu Decoction against Vascular Dementia Based on Network Pharmacology and Molecular Docking

Analysis of Potential Mechanism of Herbal Formula Taohong Siwu Decoction against Vascular Dementia Based on Network Pharmacology and Molecular Docking

Taohong Siwu Decoction Promotes Osteo-Angiogenesis in Fractures by Regulating the HIF-1α Signaling Pathway

Modified Taohong Siwu decoction improves cardiac function after myocardial ischaemia and reperfusion in rats by promoting endogenous stem cell mobilization and regulating metabolites

Contact Info

Product

Resources

About