Tapak liman (Elephantopus scaber L) Extract Induced CD4+ and CD8+ Differentiation from Hematopoietic Stem Cell/Progenitor Cell Proliferation of Mice (Mus musculus)

Djati, Muhammad Sasmito; Habibu, Hindun; Jatiatmaja, Nabilah Azzahra; Rifa’i, Muhaimin

doi:10.21776/ub.jels.2015.005.02.08

Cited by 2 publications

(3 citation statements)

References 7 publications

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“…Djati et al [23] revealed that ES leaf extracts could stimulate the proliferation of the erythroid lineage (TER119 + VLA-4 + ) in the bone marrow. The increasing level of TER119 + VLA-4 + by ES alone results from a high iron (Fe) content in the ethanol extract of ES.…”

Section: Discussionmentioning

confidence: 99%

The combination of Elephantopus scaber and Sauropus androgynus promotes erythroid lineages and modulates follicle-stimulating hormone and luteinizing hormone levels in pregnant mice infected with Escherichia coli

2021

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Background and Aim: Escherichia coli infection produces an adverse effect on the erythrocyte lineage and hormone levels during pregnancy. This study aimed to evaluate the effects of Elephantopus scaber (ES) and Sauropus androgynus (SA) in combination on circulating follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels and erythropoiesis changes in E. coli-infected pregnant mice. Materials and Methods: Female Balb/c mice were mated with normal male mice and pregnancies were identified by the formation of vaginal plugs. Twenty-eight pregnant mice were divided randomly into seven groups: A control group (N), E. coli-infected pregnant mice (K+), and infected pregnant mice received the following five treatments: (1) Only ES; (2) ESSA1 (75:25); (3) ESSA2 (50:50); (4) ESSA3 (25:75); and (5) only SA, beginning from the 1st to the 16th day of pregnancy. Pregnant mice were infected with 107 CFU/mL of E. coli on day 4. Blood serum was collected on days 8, 12, and 16 of pregnancy and LH and FSH levels were measured by enzyme-linked immunosorbent assay. Bone marrow was isolated to determine the relative number of TER-119+VLA4+ and TER-119+CD34+ using flow cytometry. Results: The ESSA1 and SA groups exhibited a marked increase in LH levels. The combination of ES and SA administered at a 25:75 ratio (ESSA3) altered FSH levels and the relative number of TER-119+VLA4+ in infected pregnant mice. Combined with SA at an equal ratio (50:50), ESSA2 group exhibited a significant increase in the expression of TER119+CD34+ compared with the other treatment groups. Conclusion: ES and SA combined at a ratio of 25:75 exhibited optimal results in altering hormonal and erythropoiesis in infected pregnant mice.

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Section: Discussionmentioning

confidence: 99%

The combination of Elephantopus scaber and Sauropus androgynus promotes erythroid lineages and modulates follicle-stimulating hormone and luteinizing hormone levels in pregnant mice infected with Escherichia coli

2021

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“…Previous studies exhibited that the effects of the Elephantopus scaber at doses of 0.5 g. 1.0 g.g -1 BW could increase the CD8 T cells number, but decrease the number of CD4 and CD8 T cells at a dose of 2.0 g.g -1 BW. The lower doses could stimulate the proliferation of T cells, while at high doses, it could prevent the T cell proliferation [19].…”

Section: Analysis the Number Of Cd8+ T Cellsmentioning

confidence: 99%

“…It is due to the content of lupeol and flavonoids, which act as an antiinflammatory agent. Previous research has also shown that the effects of herbs can stimulate or otherwise suppress immunity [19,20]. The low number of CD8 + T cells in mice after administrated by E. scaber extract probably caused by the content of isodeoxyelephantopin on Tapak Liman leaves [8].…”

Section: Analysis the Number Of Cd8+ T Cellsmentioning

confidence: 99%

Analysis Activity of Elephantopus scaber Leaves Extract Against Quantitative Changes of Lymphocytes Cells in BALB/c Mice After Induction of DMBA and Estrogen

Rochmatika¹,

Widyarti

Djati³

2019

JELS

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The carcinogenic compounds such as DMBA known for having the ability to promote the DNA damage that affects the growth regulator genes. Therefore, uncontrolled cell growth will occur and change the immune system. Elephantopus scaber extract is considered as an alternative way of handling breast cancer since it capable to affect the work of the immune system. Hence, this study aims to determine the effect of E. scaber extract on quantitative changes of CD4 + , CD8 + T cells and proinflammatory cytokines CD4 + IFN-γ + and CD4 + TNF-α + after induction of DMBA and estrogen. In vivo experiments were carried out by using 5-6 weeks old of female mice BALB/c strain. The extract of E. scaber leaf powder was obtained from Material Medica Batu, Malang. The experimental animals were divided into five groups such as negative control group (K-), positive control group (K+), mice treated with E. scaber extract for a week (group 1), mice treated with E. scaber extract for 2 weeks (group 2), and mice treated with E. scaber extract for 3 weeks (group 3). Induction of DMBA and estrogen was conducted by injection of DMBA (7.12 dimetylbenz (α) anthracene) 0.56 mg.kgBW-1 and estradiol hormone 0.0504 mg.kgBW-1. Mice were treated with E. scaber extract with a dose of 50 mg.kgBW-1. Lymphocytes were isolated from spleen and observed the CD4 + , CD8 + T cells and proinflammatory cytokines CD4 + IFN-γ + and CD4 + TNF-α + T cells through flow cytometry analysis. One-way ANOVA (p <0.05) and SPSS were used to analyze this data. The results showed that the induction of DMBA and estradiol hormone affected mice fur loss. It also decreased the immune system by lowering the number of CD4 and CD8 T cells. However, E. scaber extracts increased the number of pro-inflammatory cytokines TNF-α. Furthermore, the DMBA and estradiol induction also promoted the number of CD4 + IFN-γ + in the first week, but decreased the number of CD4 + IFN-γ + at weeks 2 and 3, then increase the number of CD4 + IFN-γ +. So, the treatment of E. scaber extracts demonstrated the ability to restore the homeostasis of the immune system in the first and second weeks.

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Tapak liman (Elephantopus scaber L) Extract Induced CD4+ and CD8+ Differentiation from Hematopoietic Stem Cell/Progenitor Cell Proliferation of Mice (Mus musculus)

Cited by 2 publications

References 7 publications

The combination of Elephantopus scaber and Sauropus androgynus promotes erythroid lineages and modulates follicle-stimulating hormone and luteinizing hormone levels in pregnant mice infected with Escherichia coli

The combination of Elephantopus scaber and Sauropus androgynus promotes erythroid lineages and modulates follicle-stimulating hormone and luteinizing hormone levels in pregnant mice infected with Escherichia coli

Analysis Activity of Elephantopus scaber Leaves Extract Against Quantitative Changes of Lymphocytes Cells in BALB/c Mice After Induction of DMBA and Estrogen

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