Tape-stripping technique

Surber, Christian; Schwarb, F. P.; Smith, Eric W.

doi:10.1081/cus-120001870

Cited by 55 publications

(49 citation statements)

References 47 publications

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“…Tape stripping was performed on the skin after the 12 h diffusion. (21). This study focused on topical drug delivery, so tape stripping was performed in order to determine the amount of drug that was retained in the layers of the skin.…”

Section: Franz Cell Diffusion Studiesmentioning

confidence: 99%

Topical Delivery of 5-Fluorouracil from Pheroid™ Formulations and the In Vitro Efficacy Against Human Melanoma

et al. 2015

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Abstract. Drug delivery vehicles can influence the topical delivery and the efficacy of an active pharmaceutical ingredient (API). In this study, the influence of Pheroid™ technology, which is a unique colloidal drug delivery system, on the skin permeation and antimelanoma efficacy of 5-fluorouracil were investigated. Lotions containing Pheroid™ with different concentrations of 5-fluorouracil were formulated then used in Franz cell skin diffusion studies and tape stripping. The in vitro efficacy of 5-fluorouracil against human melanoma cells (A375) was investigated using a flow cytometric apoptosis assay. Statistically significant concentrations of 5-fluorouracil diffused into and through the skin with Pheroid™ formulations resulting in an enhanced in vitro skin permeation from the 4.0% 5-fluorouracil lotion (p<0.05). The stratum corneum-epidermis and epidermis-dermis retained 5-fluorouracil concentrations of 2.31 and 6.69 μg/ml, respectively, after a diffusion study with the 4.0% Pheroid™ lotion. Subsequent to the apoptosis assay, significant differences were observed between the effect of 13.33 μg/ml 5-fluorouracil in Pheroid™ lotion and the effects of the controls. The results obtained suggest that the Pheroid™ drug delivery system possibly enhances the flux and delivery of 5-fluorouracil into the skin. Therefore, using Pheroid™ could possibly be advantageous with respect to topical delivery of 5-fluorouracil.

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Section: Franz Cell Diffusion Studiesmentioning

confidence: 99%

Topical Delivery of 5-Fluorouracil from Pheroid™ Formulations and the In Vitro Efficacy Against Human Melanoma

et al. 2015

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“…After its description by Pinkus [8], TS has become a standard method in dermatological research [45]. This method can be used to obtain a more susceptible skin, e.g., prior to the application of an irritant [46] or an allergen [47][48][49].…”

Section: Tape Stripping Technique Overviewmentioning

confidence: 99%

The Tape-Stripping Technique as a Method for Drug Quantification in Skin

Merino-Sanjuán²,

et al. 2008

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-Quantification of drugs within the skin is essential for topical and transdermal delivery research. Over the last two decades, horizontal sectioning, consisting of tape stripping throughout the stratum corneum, has become one of the traditional investigative techniques.Tape stripping of human stratum corneum is widely used as a method for studying the kinetics and penetration depth of drugs. This paper shows the applications of the tape stripping technique to quantify drug penetration through the skin, underlining its versatile application in the area of topical and transdermal drugs.

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“…In vivo penetration of the UV filters was determined by tape-stripping as previously described [12]. After informed consent, 6 healthy volunteers aged 25-53 years participated in this study.…”

Section: In Vivo Penetrationmentioning

confidence: 99%

Skin Penetration and Sun Protection Factor of Five UV Filters: Effect of the Vehicle

Chatelain

Gabard

Surber

2003

Skin Pharmacol Physiol

128

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To gain information about efficacy and safety of sunscreens, we compared the skin penetration of ultraviolet (UV) filters from two vehicles, i.e. an oil-in-water (O/W) emulsion gel and petrolatum jelly both in vitro and in vivo, as well as the corresponding pharmacological effect, i.e. the sun protection factor (SPF) in vivo. The UV filters studied were benzophenone-3 (BPH), ethylhexyl methoxycinnamate (EHM), butyl methoxydibenzoyl methane, ethylhexyl salicylate and homosalate. The human skin penetration of these five chemicals from the two vehicles was determined both in vitro using Franz cells and in vivo using a standardized tape-stripping method. The SPF of the two sunscreens was determined in vivo following the COLIPA guidelines. In vitro none of the filters permeated through the skin after 6 h of product application and very little could be found in the skin. BPH and EHM were the only UV filters found in the dermis (both after 30 min and 6 h). An effect of the vehicle could be noticed only for BPH after 30 min in the dermis and 6 h in both dermis and epidermis. In vivo, no differences in the amount of individual UV filters (in % of the applied dose) in the 15 first strips of the stratum corneum (SC) were found following 30 min of application of the formulations; however, the amount of UV filters that were retained in the SC was significantly higher (around 3 times) with the O/W emulsion gel than with the petrolatum jelly. This difference between the two vehicles was also of consequence for the SPF in vivo measured 30 min after application of the products (SPF ≅ 18 with the O/W emulsion gel compared to SPF ≅ 10 with the petrolatum jelly). By choosing the right vehicle or optimizing it, not only sunscreen products can be significantly improved in terms of pharmacological efficacy but the potential toxicological risk associated with the skin penetration of UV filters may be significantly reduced.

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Tape-stripping technique

Cited by 55 publications

References 47 publications

Topical Delivery of 5-Fluorouracil from Pheroid™ Formulations and the In Vitro Efficacy Against Human Melanoma

Topical Delivery of 5-Fluorouracil from Pheroid™ Formulations and the In Vitro Efficacy Against Human Melanoma

The Tape-Stripping Technique as a Method for Drug Quantification in Skin

Skin Penetration and Sun Protection Factor of Five UV Filters: Effect of the Vehicle

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