Tapetal Expression of BnaC.MAGL8.a Causes Male Sterility in Arabidopsis

Gao, Jie; Li, Qun; Wang, Nan; Tao, Baolong; Wen, Jing; Yi, Bin; Ma, Chaozhi; Tu, Jinxing; Fu, Tingdong; Li, Qiang; Zou, Jitao; Shen, Jinxiong

doi:10.3389/fpls.2019.00763

Cited by 10 publications

(8 citation statements)

References 80 publications

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“…At late microsporogenesis stages, tapetal cells undergo PCD and the precise occurrence of this process is important for tapetum degeneration and pollen development (especially for the pollen wall formation) (Sun et al, 2018b;Bai et al, 2019;Gao et al, 2019;Mondol et al, 2019;Shukla et al, 2019;Xu et al, 2019;Yang et al, 2019c;Yang et al, 2019d;Zheng et al, 2019). In both Arabidopsis and crops, GAMYB genes regulate tapetum PCD in a microRNAcontrolled manner and are believed to act under the control of gibberellic acid (GA) signaling (Kaneko et al, 2004;Millar and Gubler, 2005;Allen et al, 2007;Guo and Ho, 2008;Alonso-Peral et al, 2010;Liu et al, 2010).…”

Section: Pcd Of Tapetal Cells Is Required For Meiotic Cytokinesis and Tetrad Formationmentioning

confidence: 99%

Tapetum-Dependent Male Meiosis Progression in Plants: Increasing Evidence Emerges

Lei

Liu

2020

Front. Plant Sci.

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In higher plants, male meiosis is a key process during microsporogenesis and is crucial for male fertility and seed set. Meiosis involves a highly dynamic organization of chromosomes and cytoskeleton and specifically takes place within sexual cells. However, studies in multiple plant species have suggested that the normal development of tapetum, the somatic cell layer surrounding the developing male meiocytes, is indispensable for the completion of the male meiotic cell cycle. Disrupted tapetum development causes alterations in the expression of a large range of genes involved in male reproduction. Moreover, recent experiments suggest that small RNAs (sRNAs) present in the anthers, including microRNAs (miRNAs) and phased, secondary, small interfering RNAs (phasiRNAs), play a potential but important role in controlling male meiosis, either by influencing the expression of meiotic genes in the meiocytes or through other unclear mechanisms, supporting the hypothesis that male meiosis is non-cell autonomously regulated. In this mini review, we summarize the recorded meiotic defects that occur in plants with defective tapetum development in both Arabidopsis and crops. Thereafter, we outline the latest understanding on the molecular mechanisms that potentially underpin the tapetum-dependent regulation of male meiosis, and we especially discuss the regulatory role of sRNAs. At the end, we propose several outstanding questions that should be addressed in future studies.

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Section: Pcd Of Tapetal Cells Is Required For Meiotic Cytokinesis and Tetrad Formationmentioning

confidence: 99%

Tapetum-Dependent Male Meiosis Progression in Plants: Increasing Evidence Emerges

Lei

Liu

2020

Front. Plant Sci.

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“…In addition, a large number of sterile genes have been identified, such as BnaC. MAGL8a caused abnormal development of the tapetum layer and led to male infertility [53]. In Brassica juncea, transgenic plants expressing Arachis diogoi cysteine protease (AdCP) under the TA-29 promoter showed abnormal development of the tapetum layer [54].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the SPP/SPPL Gene Family and BnaSPPL4 Regulating Male Fertility in Rapeseed (Brassica napus L.)

Li,

Zhu,

Tian

et al. 2024

IJMS

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Signal peptide peptidase (SPP) and its homologs, signal peptide peptidase-like (SPPL) proteases, are members of the GxGD-type aspartyl protease family, which is widespread in plants and animals and is a class of transmembrane proteins with significant biological functions. SPP/SPPLs have been identified; however, the functions of SPP/SPPL in rapeseed (Brassica napus L.) have not been reported. In this study, 26 SPP/SPPLs were identified in rapeseed and categorized into three groups: SPP, SPPL2, and SPPL3. These members mainly contained the Peptidase_A22 and PA domains, which were distributed on 17 out of 19 chromosomes. Evolutionary analyses indicated that BnaSPP/SPPLs evolved with a large number of whole-genome duplication (WGD) events and strong purifying selection. Members are widely expressed and play a key role in the growth and development of rapeseed. The regulation of rapeseed pollen fertility by the BnaSPPL4 gene was further validated through experiments based on bioinformatics analysis, concluding that BnaSPPL4 silencing causes male sterility. Cytological observation showed that male infertility caused by loss of BnaSPPL4 gene function occurs late in the mononucleate stage due to microspore dysplasia.

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“…Although plant pollen is very small, it is extremely abundant in nutrients such as lipid droplets and/or starch, which are needed to ensure that there is sufficient energy and material to promote the normal germination of pollen. Any abnormal accumulation of nutrients during pollen development may cause pollen sterility ( Zhang et al ., 2002 , 2009 ; Nashilevitz et al ., 2009 ; Li et al ., 2012 ; Yang et al ., 2017 ; Gao et al ., 2019 ). As the main component of lipid stored in pollen grains, triacylglycerol (TAG) not only serves as an energy and carbon source, but it also participates in important metabolic processes such as membrane lipid remodeling and has great significance in regard to normal development and successful pollination ( Yang and Benning, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…Throughout the entire process of pollen development, the content of endoplasmic reticulum in WT tapetal cells was generally richer than that in as11 , and the lipidosomes were primarily formed by rough endoplasmic reticulum budding ( Platt et al ., 1998 ; Ting et al ., 1998 ; Hsieh and Huang, 2004 , 2005 ). The MAGL gene (a homolog of AtMAGL8 ) which encodes the monoacylglycerol lipase (MAGL) in Arabidopsis thaliana , results in male sterility, and the lipidosome content in the tapetum was higher than that in the mutant ( Gao et al ., 2019 ). Based on this, the lipidosomes that were rich in TAG in the mutants appeared later and were less abundant than those observed in the WT.…”

Section: Discussionmentioning

confidence: 99%

Changes in the content of pollen total lipid and TAG inArabidopsis thaliana DGAT1mutantas11

et al. 2023

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In mature pollen grains, lipids are primarily stored in the form of lipid droplets that provide energy and act as a carbon source for normal pollen development and germination. Triacylglycerol (TAG) is the major form of stored plant lipids. Diacylglycerol transferase, which is encoded by DGAT1 in Arabidopsis thaliana, is an important enzyme regulating triacylglycerol synthesis. Within the seeds of the DGAT1 mutant as11, the content of TAG is significantly decreased and the fatty acid composition also differs from the wild type. Transcriptome data of mature anthers showed that the genes involved in the TAG synthesis pathway were downregulated in as11. Analysis of gene expression patterns via transcriptome data also revealed that the expression of PDAT1, which functions in a manner complementary to the DGAT1 gene, was significantly decreased in as11, whereas the amylopectin synthase genes SS1 and SS2 were upregulated in mutant as11. We also detected lower total lipid, TAG and fatty acid contents in mature as11 pollen, with palmitic acid (C16:0) and linolenic acid (C18:3) being the major fatty acids in mature pollen. The cytological results showed that the lipid droplet content was reduced in mature as11 pollen. In the binuclear pollen grain II stage, WT pollen contained lipid droplets that were primarily accumulated around the generative nucleus, whereas the pollen in the mutant as11 was rich in starch grains that were primarily distributed around the vegetative nucleus. Ultrastructural analysis indicated that during pollen development in as11, the amount of endoplasmic reticulum in tapetal cells and pollen grains decreased whereas the Golgi body content increased, which directly or indirectly led to a decrease in the levels of lipidosomes and an increase in the starch content in as11. Changes in the lipid content and fatty acid composition of the mutant as11 differ from those in the wild type during pollen development.

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Tapetal Expression of BnaC.MAGL8.a Causes Male Sterility in Arabidopsis

Cited by 10 publications

References 80 publications

Tapetum-Dependent Male Meiosis Progression in Plants: Increasing Evidence Emerges

Tapetum-Dependent Male Meiosis Progression in Plants: Increasing Evidence Emerges

Genome-Wide Identification of the SPP/SPPL Gene Family and BnaSPPL4 Regulating Male Fertility in Rapeseed (Brassica napus L.)

Changes in the content of pollen total lipid and TAG inArabidopsis thaliana DGAT1mutantas11

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