Tapetum-specific expression of harpin&lt;sub&gt;Pss&lt;/sub&gt; causes male sterility in transgenic tobacco

Madhuri, B.; Raut, Sachin; Dey, Debadeepta; Nazneen, A.; Uma, Battepati; Podile, Appa Rao

doi:10.1007/s10535-012-0123-1

Cited by 5 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hybrid vigor is highly valued in agricultural industry, and thus male sterile lines are favored in generating hybrids commercially. In addition, male sterility is a trait critical for biomass production, as plant senescence proceeds much faster in fertile strains than in sterile strains [ 1 , 2 ]. Therefore, breeding new varieties of male sterile lines is not only important for the seed industry to generate hybrids at low cost but also critical for delaying senescence and increasing plant biomass.…”

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species mediate tapetal programmed cell death in tobacco and tomato

Feng

Xie

et al. 2017

BMC Plant Biol

View full text Add to dashboard Cite

BackgroundHybrid vigor is highly valued in the agricultural industry. Male sterility is an important trait for crop breeding. Pollen development is under strict control of both gametophytic and sporophytic factors, and defects in this process can result in male sterility. Both in the dicot Arabidopsis and in the moncot rice, proper timing of programmed cell death (PCD) in the tapetum ensures pollen development. Dynamic ROS levels have been reported to control tapetal PCD, and thus pollen development, in Arabidopsis and rice. However, it was unclear whether it is evolutionarily conserved, as only those two distantly related species were studied.ResultsHere, we performed histological analyses of anther development of two economically important dicot species, tobacco and tomato. We identified the same ROS amplitude during anther development in these two species and found that dynamic ROS levels correlate with the initiation and progression of tapetal PCD. We further showed that manipulating ROS levels during anther development severely impaired pollen development, resulting in partial male sterility. Finally, real-time quantitative PCR showed that several tobacco and tomato RBOHs, encoding NADPH oxidases, are preferentially expressed in anthers.ConclusionThis study demonstrated evolutionarily conserved ROS amplitude during anther development by examining two commercially important crop species in the Solanaceae. Manipulating ROS amplitude through genetic interference of RBOHs therefore may provide a practical way to generate male sterile plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1025-3) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species mediate tapetal programmed cell death in tobacco and tomato

Feng

Xie

et al. 2017

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…In wheat, optimization of the existing hybridization systems is urgently required for heterosis breeding. To date, use of heterosis in wheat production has been mainly realized through cytoplasmic male sterility (CMS)‐based breeding methods or by means of chemical hybridizing agents (CHAs) (Jordaan, 1996; Mahajan et al, 2000). Compared with CMS, an effective CHA allows the production of a great number of parental combinations and allows the evaluation of a high number of lines for combining capacity and genetic value.…”

mentioning

confidence: 99%

“…It plays an important role in regulating programmed anther development, microspore and pollen formation, and pollen wall formation (Li et al, 2006;Parish and Li, 2010). Abiotic stresses and mutation during tapetal development cause abortion of microgametogenesis, resulting in pollen sterility (Madhuri et al, 2012;Oliver et al, 2005;Phan et al, 2011;Shi et al, 2009).…”

mentioning

confidence: 99%

Carbohydrate Metabolism and Gene Regulation during Anther Development Disturbed by Chemical Hybridizing Agent in Wheat

Zhu

Zhang

et al. 2015

Crop Science

View full text Add to dashboard Cite

Induction of male sterility by chemical‐hybridizing agents is important in heterosis breeding, especially for self‐pollinated crops like wheat (Triticum aestivum L.). This study was designed to investigate the association of carbohydrate metabolism with chemical‐induced male sterility (CIMS) in wheat anthers. Transverse section observation showed that during the young microspore (YM) stage, the tapetum of fertile line anthers began to degenerate, while the CIMS line tapetum showed delayed degradation. The levels of nonreducing sugars in the CIMS line anthers always remained higher than in the fertile line, and the starch content of the CIMS line anthers was approximately 45% of those from the fertile line. Compared with the fertile line, the activities of cell wall bound invertase (CWIN) and vacuolar invertase (VIN) were significantly reduced. Quantitative real‐time polymerase chain reaction (qRT‐PCR) analyses showed that the expression levels of one CWIN gene (IVR1) and one VIN gene (IVR5) were significantly downregulated at the YM stage and the vacuolate microspore (VM) stage, and one sucrose transporter gene (TaSUT1) expression was decreased at the YM stage in CIMS anther. The results of the present work imply that sugar transport disruption is a possible crucial cause for CIMS.

show abstract

“…This enzyme is involved in the polyhydroxybutyrate (PHB) synthesis pathway, and the transgenic plants exhibited male sterility as a result of the accelerated development pattern of anthers, aberrant tissues, and collapsed pollen grains. Madhuri et al [86] developed an approach of expressing cytotoxic harpin Pss , an elicitor molecule of bacterial origin, in the tapetum of tobacco. Harpin Pss induced a hypersensitive response (HR) in the tapetum and resulted in male sterility because of premature tapetal cell death.…”

Section: Male Sterility Complete Sterility and Seed Sterilitymentioning

confidence: 99%

Can Prunus serotina be Genetically Engineered for Reproductive Sterility and Insect Pest Resistance?

Wang

Pijut

2014

Springer Science Reviews

View full text Add to dashboard Cite

Black cherry (Prunus serotina) is a valuable hardwood timber species, and its value highly depends on the wood quality which is often threatened by insect pests. Transgenic black cherry plants that are more resistant to cambial-mining insects may reduce the occurrence of gummosis and have great economic benefits to landowners and the forest products industries utilizing black cherry lumber and logs. In this review, general information about black cherry and the problem of gummosis are introduced. The various strategies for gene containment, the possibility of using cyanogenesis to enhance host resistance, and the current status of micropropagation, adventitious shoot regeneration, rooting, and Agrobacterium-mediated transformation in P. serotina and several other Prunus species are briefly discussed. Keywords Agrobacterium Á Cyanogenesis Á Flowering Á Gummosis Á In vitro culture Á Prunus Á Reproductive sterility Á Transgene containment Black Cherry Black cherry (Prunus serotina Ehrh.), also known as wild black cherry, rum cherry, and mountain black cherry, is the only member in the genus Prunus that is of commercial importance as a timber species [96]. It is native to North America and is widely distributed throughout the eastern United States [28]. There are five varieties that are usually recognized based on the height of the tree and the thickness of the leaves: (1) P. serotina var. eximia (Small) Little; (2) P. serotina var. rufula (Woot. & Standl.) McVaugh; (3) P. serotina var. virens (Woot. & Standl.) McVaugh; (4) P. serotina var. salicifolia (Kunth) Koehne; and (5) P. serotina var. serotina. Among them, P. serotina var. serotina is the most common and widespread variety in the eastern United States and Canada [126]. Black cherry is one of the most valuable hardwoods for cabinets, furniture, veneer, architectural millwork, and musical instruments [93]. However, large and high-quality trees suited for commercial use (belonging to var. serotina) are only found in a restricted area on the Allegheny Plateau of Pennsylvania, New York, and West Virginia [57, 92]. Black cherry (subgenus Padus) is a deciduous and monoecious tree with moderate size. The leaves are 5-13cm in length and elliptical with serrated leaf margins. Its elongated racemes are small and have numerous white, perfect flowers in a leafy shoot, and its fruits are fleshy [14]. The fruits are acyanogenic throughout the developmental stages as they lack the catabolic enzymes, whereas the seeds become highly cyanogenic during the process of maturation with the accumulation of both the catabolic enzymes and cyanogenic glycosides [166]. Black cherry grows best in areas that are cool, moist, and temperate [41]. Black cherry serves as a host to a large number of pathogens. It is susceptible to soil-borne pathogens that are Endorsed by Paula M. Pijut.

show abstract

Tapetum-specific expression of harpin<sub>Pss</sub> causes male sterility in transgenic tobacco

Cited by 5 publications

References 21 publications

Reactive oxygen species mediate tapetal programmed cell death in tobacco and tomato

Reactive oxygen species mediate tapetal programmed cell death in tobacco and tomato

Carbohydrate Metabolism and Gene Regulation during Anther Development Disturbed by Chemical Hybridizing Agent in Wheat

Can Prunus serotina be Genetically Engineered for Reproductive Sterility and Insect Pest Resistance?

Contact Info

Product

Resources

About