1998
DOI: 10.1074/jbc.273.40.25889
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Tarantula Hemocyanin Shows Phenoloxidase Activity

Abstract: An enzyme generally catalyzes one well defined reaction with high specificity and efficiency. We report here in contrast that the copper protein hemocyanin of the tarantula Eurypelma californicum exhibits two different functions. These occur at the same active site. While hemocyanin usually is an oxygen carrier, its function can be transformed totally to monophenoloxidase and o-diphenoloxidase activity after limited proteolysis with trypsin or chymotrypsin. N-acetyldopamine (NADA) is more effectively oxidized … Show more

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Cited by 205 publications
(148 citation statements)
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“…These experimental observations suggest that CuðIIÞ 2 ðμ-η 2 ∶η 2 -O 2 2− Þ cores are intrinsically able to perform all of the CBC protein functions. Consistent with this hypothesis was the recent observation that partial denaturation or proteolytic digestion of hemocyanin induces monophenolase activity (14,15).…”
supporting
confidence: 67%
“…These experimental observations suggest that CuðIIÞ 2 ðμ-η 2 ∶η 2 -O 2 2− Þ cores are intrinsically able to perform all of the CBC protein functions. Consistent with this hypothesis was the recent observation that partial denaturation or proteolytic digestion of hemocyanin induces monophenolase activity (14,15).…”
supporting
confidence: 67%
“…If hemocyanins did evolve from phenoloxidases and tyrosinases the substrate binding capacity of the enzyme must have been inhibited without hindering O 2 binding. This seems to have been accomplished by the addition of residues blocking the active site to large substrates (17,23,24). Indeed, just such a difference has been noted by comparing the crystal structure of sweet potato catechol oxidase with that of molluscan hemocyanin (15).…”
Section: From What Did Hemocyanins Evolve?mentioning
confidence: 99%
“…Under certain conditions, such as limited proteolysis or binding to small molecules (e.g., SDS), hemocyanins can be converted to enzymes displaying PO activities (30,31). Although o-di-PO activity of hemocyanins was observed most of the time (32), mono-oxygenase activity was observed from hemocyanins of a scorpion (33), a tarantula (34), and an octopus (35).…”
mentioning
confidence: 99%