Taraxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-κB modulation in RAW 264.7 cells

Park, Chung Mu; Park, Ji Young; Noh, Kyung Hee; Shin, Jin Hyuk; Song, Young Sun

doi:10.1016/j.jep.2010.11.015

Cited by 101 publications

(55 citation statements)

References 44 publications

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“…A wide range concentrations of LPS (10-10000 ng/mL) [21,27,28] can induce oxidative stress in macrophage of mice. LPS from different bacterial sources will not always elicit identical biological responses, especially when tested in different target species.…”

Section: Discussionmentioning

confidence: 99%

“…Lipopolysaccharide (LPS) is a component of cell walls and the major virulence factor of gram-negative bacteria. Oxidative stress can be induced by LPS in mouse macrophages [2,3] and in rats [4].…”

Section: Introductionmentioning

confidence: 99%

“…There are reports [21][22][23] that 1 μg/mL of LPS induces oxidative stress in macrophages of mice. Preliminary testing with different concentrations of LPS (0, 100, 400 ng/mL) to induce oxidative stress in chicken macrophages found that 100 ng/mL of LPS was the most effective (data not shown).…”

Section: Introductionmentioning

confidence: 99%

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Equol Inhibits LPS-Induced Oxidative Stress and Enhances the Immune Response in Chicken HD11 Macrophages

Gou

Jiang

Zheng

et al. 2015

Cell Physiol Biochem

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Background/Aims: There has been increasing recent attention on the antioxidative capacity of equol. This study tested the effect of equol on oxidative stress induced by lipopolysaccharide (LPS) and regulation of immunity in chicken macrophages. Methods: Chicken HD11 macrophages were challenged with LPS (100 ng/mL) alone or with LPS (100 ng/mL) and (±)equol (10, 20, 40, 80, 160 μmol/L) together for 24h. Evaluated responses included the contents of malondialdehyde (MDA) and reduced glutathione (GSH), activities of total superoxide dismutase (T-SOD) and inducible nitric oxide synthase (iNOS), transcript abundance of superoxide dismutase 2 (SOD2), catalase (CAT), glutathione transferase (GST), Toll-like receptor 4 (TLR4), tumor necrosis factor alpha (TNFα) and interleukin-1 beta (IL-1β), and contents of the cytokines TNFα, IL-1β, interleukin-2 (IL-2) and interferon beta (IFNβ). Results: Exposure to LPS induced oxidative stress as contents of MDA increased and GSH decreased in LPS-treated cells (P < 0.05) compared to those in control cells. Compared to LPS alone, co-treatment with equol (20 μmol/L, 40 μmol/L or 80 μmol/L) reduced contents of MDA and increased those of GSH (both P < 0.05). Activity of T-SOD increased (P < 0.05) in cells treated with the higher contentration of equol (80 μmol/L or 160 μmol/L), however, all concentrations (20 μmol/L to 160 μmol/L) increased activity of iNOS (P < 0.05). The highest concentration of equol (160 μmol/L) increased SOD2 and GST transcripts (P < 0.05). Equol treatment increased transcripts of TLR4, TNFα and IL-1β (P < 0.05). And there were similar changes in contents of IL-1β, IL-2, IFNβ and TNFα in the cells (P < 0.05). Conclusions: It concluded that equol can protect chicken HD11 macrophages from oxidative stress induced by LPS through reducing lipid peroxidation products and enhancing contents of antioxidants, and activities of relevant antioxidase enzymes; effects were also seen in gene expression related to the immune response and increased contents of cytokines. The optimal concentration of equol on antioxidation and immune enhancement in chicken macrophages was 40 μmol/L.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Equol Inhibits LPS-Induced Oxidative Stress and Enhances the Immune Response in Chicken HD11 Macrophages

Gou

Jiang

Zheng

et al. 2015

Cell Physiol Biochem

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“…Separate but simultaneous PCR amplifications were performed with aliquots of cDNA (10 µl) at a final concentration of 1 × PCR buffer, 4 mM MgCl 2 , 400 µM dNTPs, and 2 U Taq DNA polymerase (TaKaRa, Dalian, China) in a total volume of 20 µl using 1 µl (20 µM) each of forward and reverse primers. The primer sequences of iNOS were selected according to Park et al [28]. The sequences of GPx, GR, Cu/Zn SOD, Mn SOD, and CAT were selected according to Ferret et al [29].…”

Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

The Suppressive Effects of Bursopentine (BP5) on Oxidative Stress and NF-ĸB Activation in Lipopolysaccharide-activated Murine Peritoneal Macrophages

Mao-yun

Geng

et al. 2012

Cell Physiol Biochem

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Background/Aim: Bursopentine (BP5) is a novel thiol-containing pentapeptide isolated from chicken bursa of Fabricius, and is reported to exert immunomodulatory effects on B and T lymphocytes. It has been found that some thiol compounds, such as glutathione (GSH) and N-acetylcysteine (NAC) protect living cells from oxidative stress. This led us to investigate whether BP5 had any ability to protect macrophages from oxidative stress as well as any mechanism that might underlie this process. Methods: Murine peritoneal macrophages activated by lipopolysaccharide (LPS) (2 µg/ml) were treated with single bouts (0, 25, 50, and 100 µM) of BP5. Results: BP5 potently suppressed the markers for oxidative stress, including nitric oxide (NO), reactive oxygen species (ROS), lipid peroxidation, and protein oxidation. It also decreased the expression and activity of inducible nitric oxide synthase (iNOS) and promoted a protective antioxidant state by elevating GSH content and by activating the expression and activity of certain key antioxidant and redox enzymes, including glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT). This suppressive effect on oxidative stress was accompanied by down-regulated expression and activity of nuclear factor kappa B (NF-ĸB). Conclusion: These findings demonstrate that BP5 can protect LPS-activated murine peritoneal macrophages from oxidative stress. BP5 may have applications as an anti-oxidative stress reagent.

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“…For example, chromatographic analysis of a dandelion flower ethyl acetate fraction revealed the presence of luteolin, luteolin-7-O-glycoside, caffeic acid and chlorogenic acid, 14,15 while the luteolin, chicoric and total phenolic content leaf extracts has also been measured spectrophotometrically. 16 In the root itself, the presence of phenolic compounds such as chicoric acid, chlorogenic acid and monocaffeyltartaric acid have been reported from commercial root coffee and capsules. 17 In the present study we have adopted a sequential extraction approach in combination with molecular weight cut off (MWCO) dialysis in an attempt to reveal more detailed information about the antioxidant activity and phenolic content of multiple crude extracts.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant properties and quantitative UPLC-MS/MS analysis of phenolic compounds in dandelion (Taraxacum officinale) root extracts

Kenny

Smyth

Hewage³

et al. 2014

Free Rad. Antiox.

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Taraxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-κB modulation in RAW 264.7 cells

Cited by 101 publications

References 44 publications

Equol Inhibits LPS-Induced Oxidative Stress and Enhances the Immune Response in Chicken HD11 Macrophages

Equol Inhibits LPS-Induced Oxidative Stress and Enhances the Immune Response in Chicken HD11 Macrophages

The Suppressive Effects of Bursopentine (BP5) on Oxidative Stress and NF-ĸB Activation in Lipopolysaccharide-activated Murine Peritoneal Macrophages

Antioxidant properties and quantitative UPLC-MS/MS analysis of phenolic compounds in dandelion (Taraxacum officinale) root extracts

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