Target‐Induced and Equipment‐Free DNA Amplification with a Simple Paper Device

Li, Meng; Hui, Christy Y.; Zhang, Qiang; Gu, Jimmy; Kannan, Balamurali; Jahanshahi‐Anbuhi, Sana; Filipe, Carlos D. M.; Brennan, John D.; Li, Yingfu

doi:10.1002/ange.201509389

Cited by 52 publications

(27 citation statements)

References 57 publications

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“…63,64 However, although these types of test are appropriate for immunoassays, their application for nucleic acid detection is more challenging. Although isothermal amplification assays can be conducted on paper, 65 these tests are generally for nucleic acid detection alone, whereas quantitative analysis of nucleic acid concentration has yet to be achieved.…”

Section: Emerging Technologiesmentioning

confidence: 99%

Genome‐wide host RNA signatures of infectious diseases: discovery and clinical translation

et al. 2017

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SummaryThe use of whole blood gene expression to derive diagnostic biomarkers capable of distinguishing between phenotypically similar diseases holds great promise but remains a challenge. Differential gene expression analysis is used to identify the key genes that undergo changes in expression relative to healthy individuals, as well as to patients with other diseases. These key genes can act as diagnostic, prognostic and predictive markers of disease. Gene expression ‘signatures’ in the blood hold the potential to be used for the diagnosis of infectious diseases, where current diagnostics are unreliable, ineffective or of limited potential. For diagnostic tests based on RNA signatures to be useful clinically, the first step is to identify the minimum set of gene transcripts that accurately identify the disease in question. The second requirement is rapid and cost‐effective detection of the gene expression levels. Signatures have been described for a number of infectious diseases, but ‘clinic‐ready’ technologies for RNA detection from clinical samples are limited, though existing methods such as RT‐PCR are likely to be superseded by a number of emerging technologies, which may form the basis of the translation of gene expression signatures into routine diagnostic tests for a range of disease states.

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Section: Emerging Technologiesmentioning

confidence: 99%

Genome‐wide host RNA signatures of infectious diseases: discovery and clinical translation

et al. 2017

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“…Chemical biology, drug development, medical diagnosis and environmental monitoring have employed a large number of biological assays. 1 – 5 Many of these assays use labile enzymes and bioreactive organic molecules to generate easily-tracked signals such as color changes, 6 fluorescence, chemiluminescence 7 and electrochemical readouts. 8 , 9 For such assays, maintaining the long-term stability of enzymes and signal-generating small molecules remains a significant challenge, which is particularly important in the context of point-of care-diagnostics, where reagents need to be used in the field and far away from the well-controlled conditions of a laboratory.…”

Section: Introductionmentioning

confidence: 99%

Simple and ultrastable all-inclusive pullulan tablets for challenging bioassays

et al. 2016

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“…Significant efforts have been devoted to the development of rapid quantitative readouts in POCT assays, such as optical detection [21][22][23][24][25] , electrochemical detection [26][27][28][29] , and commercial portable instrument-based detection [30][31][32][33] . While these methods are sensitive and user-friendly, most of them are unsuitable for application with the broad population.…”

Section: Introductionmentioning

confidence: 99%

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

et al. 2018

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Herein, we demonstrate a contact angle (CA)-based naked-eye point-of-care testing platform with rapid pH-responsive superwettability that can switch between superhydrophilic and superhydrophobic properties for quantitative biosensing. The CA of the droplet on the pH-responsive surface approached~0°at pH 1 and conversely reached 161.4°± 6.2°at pH 13. We realized the sensitive detection of the pH, urea, and glucose by monitoring the changes in the CA. The traditional invasive diagnosis of diabetes causes pain and brings the risk of infections, such as acquired immune deficiency syndrome (AIDS), hepatitis B, hepatitis C, and syphilis, to the user. To address this issue, we implemented a method for the non-invasive diagnosis of diabetes in human saliva and urine, which avoided the significant drawbacks mentioned above. The accuracy of this method was demonstrated by comparing the results with those from commercial glucometers and theoretical calculations. Interestingly, we successfully monitored glucose levels in sweat before, during, and after cycling. The sensing performance was barely influenced by the temperature, elevation, and droplet color, illustrating promise for expansion to hundreds of millions of potential customers, especially those with color blindness or color weakness. Given its low cost, lack of instruments, and rapid response (within 1 s), this strategy might overcome the limitations of the mechanical stability and durability of superwettable materials and thus might extend the industrial-scale application of bioinspired superwettable systems.

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Target‐Induced and Equipment‐Free DNA Amplification with a Simple Paper Device

Cited by 52 publications

References 57 publications

Genome‐wide host RNA signatures of infectious diseases: discovery and clinical translation

Genome‐wide host RNA signatures of infectious diseases: discovery and clinical translation

Simple and ultrastable all-inclusive pullulan tablets for challenging bioassays

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

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