Target-site basis for fomesafen resistance in redroot pigweed (<i>Amaranthus retroflexus</i>) from China

Du, Long; Xiao, Li; Jiang, Xiaojing; Ju, Qian; Guo, Wenlei; Li, Lingxu; Qu, Chunjuan; Qu, Mingjing

doi:10.1017/wsc.2021.14

Cited by 7 publications

(17 citation statements)

References 46 publications

(75 reference statements)

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“…The effect of ΔG210 and R128G mutations on PPO activity has already been investigated and our results somewhat agrees with previous studies. 15,16,18,25 These mutations are known to affect PPO activity and substrate binding differently. While ΔG210 did not affect substrate binding (no differences in Km between WT and mutant), the mutation reduced enzyme activity by a factor of 10.…”

Section: Evaluation Of Double-mutant R128g δG210 Ppo2 Enzymatic Activitymentioning

confidence: 99%

“…In addition, the substitution of the bulky, charged Arg to the small, non-polar Gly, increases the opening of the binding pocket, further speeding the proto egress. 18,25 To confirm the double-mutant viability, Amaranthus palmeri PPO2 carrying R128G ΔG210 was overexpressed in Arabidopsis and the resulting transgenic plants were treated with two rates of saflufenacil (10 and 25 g ha -1 ). If active, transgenic plants expressing R128G ΔG210 should be resistant to saflufenacil.…”

Section: Evaluation Of Double-mutant R128g δG210 Ppo2 Enzymatic Activitymentioning

confidence: 99%

“…In contrast, R128G impairs protein binding efficacy, but doubles its activity. 18 The decrease in substrate binding is caused by the loss of the salt bridge interaction between R128 and protogen. However, this also facilitates the release of proto from the binding site.…”

Section: Evaluation Of Double-mutant R128g δG210 Ppo2 Enzymatic Activitymentioning

confidence: 99%

“…In addition, the substitution of the bulky, charged Arg to the small, non-polar Gly, increases the opening of the binding pocket, further speeding the proto egress. 18,25 should be resistant to saflufenacil. The R128G ΔG210 Arabidopsis transgenics did not survive the field dose of saflufenacil, indicating that protein activity of this variant is too low to confer herbicide tolerance (Fig 2).…”

Section: Evaluation Of Double-mutant R128g δG210 Ppo2 Enzymatic Activitymentioning

confidence: 99%

“…12 This mutation led to a 97% reduction in enzyme activity compared to the WT PPO2. Lastly, mutations at the Arg128 position (homologous to Arg98, in Ambrosia artemisiifolia) has been reported in several species, such as A. tuberculatus, 10 A. palmeri, 17 A. retroflexus, 18,19 A. artemisiifolia, 20 and Euphorbia heterophylla. 21 The most common substitution is R128G (4 species), while R128M, R128I and R128L are present in only one species each (A. palmeri, A. tuberculatus and A. artemisiifolia, respectively).…”

Section: Introductionmentioning

confidence: 99%

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Can double PPO mutations exist in a same allele and are such mutants functional?

Porri

Roma‐Burgos²

2022

Preprint

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Background: Resistance to PPO-inhibiting herbicides is primarily endowed by target site mutations at the PPO2 gene that compromise binding of the herbicide to the catalytic domain. In Amaranthus ssp. PPO2, the most prevalent target mutations are deletion of the G210 codon and the R128G and G339A substitutions. These mutations strongly affect the dynamic of the PPO2 binding pocket resulting in reduced affinity with the ligand. Here we investigated the likelihood of co-occurrence of the most widespread target site mutations in the same ppo2 allele. Results: Plants carrying R128G+/+ ΔG210+/-, where + indicates presence of the mutation, were crossed with each other. The ppo2 of the offspring was subjected to pyrosequencing and E. coli- based Sanger sequencing to determine mutation frequencies and allele co-occurrence. The data show that R128G ΔG210 can occur only in one allele; the second allele carries only one mutation. Double mutation in both alleles is less likely because of significant loss of enzyme activity. The segregation of offspring populations derived from a cross between heterozygous plants carrying ΔG210 G399A also showed no co-occurrence in the same allele. The offspring exhibited the expected mutation distribution patterns with few exceptions. Conclusions: Homozygous double-mutants are not physiologically viable. Double-mutant plants can only exist in a heterozygous state. Alternatively, if two mutations are detected in one plant, each mutation would occur in a separate allele

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Section: Evaluation Of Double-mutant R128g δG210 Ppo2 Enzymatic Activitymentioning

confidence: 99%

Section: Evaluation Of Double-mutant R128g δG210 Ppo2 Enzymatic Activitymentioning

confidence: 99%

Section: Evaluation Of Double-mutant R128g δG210 Ppo2 Enzymatic Activitymentioning

confidence: 99%

Section: Evaluation Of Double-mutant R128g δG210 Ppo2 Enzymatic Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Can double PPO mutations exist in a same allele and are such mutants functional?

Porri

Roma‐Burgos²

2022

Preprint

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Can double PPO mutations exist in the same allele and are such mutants functional?

Porri

Noguera

Betz

et al. 2022

Pest Management Science

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BACKGROUND: Resistance to protoporphyrinogen oxidase (PPO)-inhibiting herbicides is endowed primarily by target-site mutations at the PPX2 gene that compromise binding of the herbicide to the catalytic domain. In Amaranthus spp. PPX2, the most prevalent target mutations are deletion of the G210 codon, and the R128G and G339A substitutions. These mutations strongly affect the dynamic of the PPO2 binding pocket, resulting in reduced affinity with the ligand. Here we investigated the likelihood of co-occurrence of the most widespread target site mutations in the same PPX2 allele. RESULTS: Plants carrying R128G+/+ ΔG210+/−, where + indicates presence of the mutation, were crossed with each other. The PPX2 of the offspring was subjected to pyrosequencing and E. coli-based Sanger sequencing to determine mutation frequencies and allele co-occurrence. The data show that R128G ΔG210 can occur in one allele only; the second allele carries only one mutation. Double mutation in both alleles is less likely because of significant loss of enzyme activity. The segregation of offspring populations derived from a cross between heterozygous plants carrying ΔG210 G399A also showed no co-occurrence in the same allele. The offspring exhibited the expected mutation distribution patterns with few exceptions. CONCLUSIONS: Homozygous double-mutants are not physiologically viable. Double-mutant plants can only exist in a heterozygous state. Alternatively, if two mutations are detected in one plant, each mutation would occur in a separate allele.

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Herbicidal activity and biochemical characteristics of the botanical drupacine against Amaranthus retroflexus L.

TIAN

SHEN

et al. 2023

Journal of Integrative Agriculture

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Target-site basis for fomesafen resistance in redroot pigweed (Amaranthus retroflexus) from China

Cited by 7 publications

References 46 publications

Can double PPO mutations exist in a same allele and are such mutants functional?

Can double PPO mutations exist in a same allele and are such mutants functional?

Can double PPO mutations exist in the same allele and are such mutants functional?

Herbicidal activity and biochemical characteristics of the botanical drupacine against Amaranthus retroflexus L.

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