Targeted detection of 65kDa heat shock protein gene in endometrial biopsies for reliable diagnosis of genital tuberculosis

Prasad, Sudha; Singhal, Megha; Negi, Sanjay Singh; Gupta, Sunil; Singh, Sukhbir; Rawat, Devendra Singh; Rai, Arvind

doi:10.1016/j.ejogrb.2011.11.015

Cited by 23 publications

(27 citation statements)

References 20 publications

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“…10 (26%), the positive results were lower in this study. The study by Kohli et al 11 (13%) and Prasad et al 12 (14.6%) showed low positivity as compared with the present study.…”

Section: Discussioncontrasting

confidence: 51%

Does Thin Endometrium in Infertile Women Indicate Genital Tuberculosis?

Pai¹,

Roopa²,

Kumar³

2016

jemds

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BACKGROUNDFemale genital tuberculosis (FGTB) primarily an asymptomatic disease, is one of the most important causes of female infertility in developing countries. The diagnosis of GTB has been a challenge. In recent years, polymerase chain reaction (PCR) technique has evolved as a useful and rapid technique for the diagnosis of GTB, but this method has some limitations. Ultrasound of the endometrium is easy to perform and therefore the imaging modality of choice in a clinical setting, however its role in diagnosing genital tuberculosis has not been explored to a large extent.The aim of the study was to determine whether thin (<8mm) endometrium in infertile women indicates genital tuberculosis (GTB) and to ascertain the validity of thin (<8mm) endometrium in the diagnosis of genital tuberculosis.

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“…10 (26%), the positive results were lower in this study. The study by Kohli et al 11 (13%) and Prasad et al 12 (14.6%) showed low positivity as compared with the present study.…”

Section: Discussioncontrasting

confidence: 51%

Does Thin Endometrium in Infertile Women Indicate Genital Tuberculosis?

Pai¹,

Roopa²,

Kumar³

2016

jemds

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“…In an earlier study, we used 1H NMR metabonomics and could successfully characterize the metabolic profile of the endometrium obtained from women with GTB and healthy controls 17 . This study revealed the possible association of dysregulated energy metabolism and amino acid biosynthesis metabolites with repeated implantation failure in dormant GTB women 4 .…”

Section: Introductionmentioning

confidence: 77%

“…Importantly, GTB is associated with implantation failure, even in its dormant form 2, 3 . The 65 kDa heat shock protein (HSP65), secreted by Mycobacterium tuberculosis during intracellular survival, is one of the major immunologically active antigens and considered to be a diagnostic marker of GTB 4 . This protein plays an important role in the pathogenesis of tuberculosis 4, 5 and may contribute to the implantation failure in GTB women.…”

Section: Introductionmentioning

confidence: 99%

Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells

Subramani

Rameshbabu

Jothiramajayam

et al. 2017

Sci Rep

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Successful implantation is dependent on the appropriate decidualization of endometrial stromal cells for the establishment of pregnancy in women. Mycobacterial heat shock protein 65 (HSP65) is involved in pathogenesis of the genital tuberculosis (GTB), one of the common causes of infertility in emerging countries. Though implantation failure appears to be the major cause, understanding the status of decidualizaiton process in women diagnosed with GTB has not been thoroughly addressed. We, therefore, explored the effect of HSP65 protein on the endometrial cell metabolism during in vitro decidualization. In order to identify the cellular metabolism of decidual cells with and without HSP65 treatment, proton NMR based characterization of metabolites extracted from cells and culture media were performed. In presence of HSP65, significant reduction in the decidual phenotype of endometrial stromal cells and prolactin expression is suggestive of impairment in decidualization. The intracellular and extracellular metabolic changes in HSP65 treated endometrial stromal cells produced a distinct pattern, reflecting the interaction between the protein and cellular metabolism. HSP65 mediated dysregulation in cellular metabolism is associated with poor decidualization. Besides enriching the present knowledge on metabolic changes underlying stromal cells decidualization, these findings assist in identifying potential molecular causes for decidualization failure in GTB women.

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“…According to Prasad et al (2012), a total of 22 (14.6%) women were diagnosed with genital tuberculosis on the basis of laboratory tests and laparoscopic/hysteroscopic findings.…”

Section: Discussionmentioning

confidence: 99%

To evaluate the prevalence of female genital tuberculosis in Hyderabad

Rao

Pavani

et al. 2013

Int J Res Med Sci

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Background: Tuberculosis (TB) is an increasing public health concern worldwide. It is one of the most important causes of infectious morbidity and mortality. Genital TB is one form of extra pulmonary TB and is not uncommon, particularly in communities where pulmonary TB is prevalent. The objective of the study was to evaluate the prevalence of female genital tuberculosis in Hyderabad and its presentational symptoms and methods of diagnosis. Methods: A total of 1102 cases of suspected genital TB who had been registered and treated in the AIMSR and few selected government urban health post and private hospitals and clinics in different zones of Hyderabad, from 2012 January to 2013 March were retrospectively and prospectively studied. From this group, 23 women were diagnosed as having genital TB based on the standard pathological and microbiological criteria of tissue specimens. Results: Out of 23 cases diagnosed as having genital tuberculosis, 3 patients (13.04 %) presented with abdominal or pelvic pain. In 17cases (73.91%) tuberculosis was diagnosed during studies performed to evaluate the cause of their infertility, and the most common diagnostic procedure was endometrial curettage. Remaining 3 cases (13.05 %) have past, present or contact history of tuberculosis. Female genital TB accounted for 2.08 % of all tuberculosis patients in this study. Conclusions: This study indicates the presence of a strong relationship between genital TB and infertility; therefore genital TB would be more frequently diagnosed if this possibility was considered in the evaluation of every infertile patient in areas where tuberculosis is endemic. [Int J Res Med Sci 2013; 1(4.000): 421-423

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Targeted detection of 65kDa heat shock protein gene in endometrial biopsies for reliable diagnosis of genital tuberculosis

Cited by 23 publications

References 20 publications

Does Thin Endometrium in Infertile Women Indicate Genital Tuberculosis?

Does Thin Endometrium in Infertile Women Indicate Genital Tuberculosis?

Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells

To evaluate the prevalence of female genital tuberculosis in Hyderabad

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