Targeted doxorubicin delivery to hepatocarcinoma cells by lactobionic acid-modified laponite nanodisks

Abstract

Lactobionic acid-modified laponite can deliver doxorubicin specifically to hepatocarcinoma cells overexpressing the asialoglycoprotein receptor and display a significantly enhanced therapeutic efficacy.

Capturing hepatocellular carcinoma cells using lactobionic acid-functionalized electrospun polyvinyl alcohol/polyethyleneimine nanofibers

“…The peak at 3.5 ppm can be assigned to the PEG -CH 2 -protons, and the characteristic proton peaks at 3.4 to 4.3 ppm are associated to the LA moieties, confirming the successful PEGylation of LA, in agreement with 45 our previous reports. 43,44 Based on NMR peak integration associated to the NH 2 -PEG-COOH and the LA, the number of LA moieties attached to each NH 2 -PEG-COOH was estimated to be 1.0.…”

“…This suggest that the LAmodification onto the nanofibers is able to greatly enhance the capture ability of the fibrous mat presumably through the specific 40 targeting of LA to ASGPR-overexpressing HCC cells. It is also noted that the efficiency of unmodified crosslinked PVA/PEI nanofibrous mats to capture HepG2 cells increases with the incubation time ( Figure S2, ESI), and at 240 min, the cell capture efficiency reaches 70.37%, which is quite similar to that of the 45 mPEG-PVA/PEI-Ac nanofibrous mat (73.06%). In contrast, for HeLa cells without ASGPR expression, almost the same capture efficiency can be achieved by the mPEG-PVA/PEI-Ac and the LA-PEG-PVA/PEI-Ac nanofibrous mat at the same time point (Figure 7b).…”

“…Then, the two ends of sample were gripped in the top and bottom chucks, respectively, and the gauge of two chucks connected to the testing machine was fixed at 30 mm. The stress and strain data were calculated 45 using Equations 1 and 2:…”

“…4 35 Electrospinning has been recognized as a versatile and powerful technique to fabricate ultralong synthetic or natural polymer nanofibers with fiber diameter ranging from tens of nanometers to several micrometers. [5][6][7][8] The intriguing physicochemical properties of electrospun nanofibers such as 40 extremely large surface area to volume ratio, high porosity, superior mechanical durability, similar dimensions to cellular surface components (e.g., microvilli and filopodia), and capability to mimic the extracellular matrix (ECM) enable them to be widely used in the fields of tissue engineering [9][10][11][12][13] and drug 45 delivery. 14- 17 Recently, electrospun nanofibers have been employed as substrates to capture circulating tumor cells (CTCs).…”

Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems

“…LA has been used to functionalize a number of different carriers such as magnetite nanoparticles (Song, et al, 2015) and laponite (a synthetic clay) (Chen, et al, 2015) for targeted drug delivery, or dendrimer-entrapped gold nanoparticles for computed tomography imaging applications . However, the possibility of using functionalized GO to target hepatocytes via this route has not been explored.…”