Targeted dual-color silica nanoparticles provide univocal identification of micrometastases in preclinical models of colorectal cancer

Soster, Marco; Juris, Riccardo; Bonacchi, Sara; Genovese, Damiano; Montalti, Marco; Rampazzo, Enrico; Zaccheroni, Nelsi; Garagnani, Paolo; Bussolino, Federico; Prodi, Luca; Marchiò, Serena

doi:10.2147/ijn.s33825

Cited by 17 publications

(7 citation statements)

References 15 publications

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“…In particular, in vivo imaging is the leading biological application of FSNs [17,18]. The encapsulation of designated fluorophores determines the optical properties of the FSNs and their application, such as multicolor imaging [19] or NIR imaging [20,21]. As we expected, when fluorophores are incorporated into silica nanoparticles, FSN possesses a better photostability because the silica matrix (or shell) protects the fluorophores from oxygen and other harmful species, which may cause photobleaching.…”

Section: Introductionmentioning

confidence: 92%

Fabrication of Double Emission Enhancement Fluorescent Nanoparticles with Combined PET and AIEE Effects

Chang

2020

Molecules

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The major challenge in the fabrication of fluorescent silica nanoparticles (FSNs) based on dye-doped silica nanoparticles (DDSNs) is aggregation-caused fluorescence quenching. Here, we constructed an FSN based on a double emission enhancement (DEE) platform. A thio-reactive fluorescence turn-on molecule, N-butyl-4-(4-maleimidostyryl)-1,8-naphthalimide (CS), was bound to a silane coupling agent, (3-mercaptopropyl)-trimethoxysilane (MPTMS), and the product N-butyl-4-(3-(trimethoxysilyl-propylthio)styryl)-1,8-naphthalimide (CSP) was further used to fabricate a core–shell nanoparticle through the Stöber method. We concluded that the turn-on emission by CSP originated from the photoinduced electron transfer (PET) between the maleimide moiety and the CSP core scaffold, and the second emission enhancement was attributed to the aggregation-induced emission enhancement (AIEE) in CSP when encapsulated inside a core–shell nanoparticle. Thus, FSNs could be obtained through DEE based on a combination of PET and AIEE effects. Systematic investigations verified that the resulting FSNs showed the traditional solvent-independent and photostable optical properties. The results implied that the novel FSNs are suitable as biomarkers in living cells and function as fluorescent visualizing agents for intracellular imaging and drug carriers.

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Section: Introductionmentioning

confidence: 92%

Fabrication of Double Emission Enhancement Fluorescent Nanoparticles with Combined PET and AIEE Effects

Chang

2020

Molecules

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“…Indeed, efficacy of treatment can be radically improved through earlier detection of metastatic foci, which depends on sensitivity and specificity of the analytic tool. Soster and colleagues recently developed Rhodamine and/or Cy5 fluorescent silica-poly(ethylene glycol) nanoparticles (SPNs) coupled to two metastasis-specific peptides [64 ]. A poly(ethylene glycol) (PEG) shell embeds a dye-bound silica core, and presents carbonyl groups to enable covalent attachment of targeting ligands, such as antibodies, proteins, or short peptides.…”

Section: 2 Nanomaterials For Labeling and In Vivo Tracking Of Stemmentioning

confidence: 99%

Stem Cells and Nanomaterials

Hofmann

2014

Advances in Experimental Medicine and Biology

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Because of their ability to self-renew and differentiate into many cell types, stem cells offer the potential to be used for tissue regeneration and engineering. Much progress has recently been made in our understanding of the biology of stem cells and our ability to manipulate their proliferation and differentiation to obtain functional tissues. Similarly, nanomaterials have been recently developed that will accelerate discovery of mechanisms driving stem cell fate and their utilization in medicine. Nanoparticles have been developed that allow the labeling and tracking of stem cells and their differentiated phenotype within an organism. Nanosurfaces are engineered that mimic the extracellular matrix to which stem cells adhere and migrate. Scaffolds made of functionalized nanofibers can now be used to grow stem cells and regenerate damaged tissues and organs. However, the small scale of nanomaterials induces changes in their chemical and physical properties that might modify their interactions with cells and tissues, and render them toxic to stem cells. Therefore a thorough understanding of stem cell-nanomaterial interactions is still necessary not only to accelerate the success of medical treatments but also to ensure the safety of the tools provided by these novel technologies.

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“…64 The deriving nanoprobes selectively homed to and accumulated at target tissues, resulting in specific visualization even of submillimetric metastatic foci . 40 Other examples of silica nanoparticles containing more than one fluorophoric species are present in literature to allow the simultaneous imaging of multiple targets. In particular W. Tan and co-authors 65 proposed the use of fluorescence resonance energy transfer (FRET) NPs conjugated with aptamers to perform multiplexed cancer cell monitoring.…”

Section: Imaging With Silica-based Nanoparticles In Cancermentioning

confidence: 99%

Luminescent Silica Nanoparticles for Cancer Diagnosis

Arap¹,

Pasqualini²,

Montalti³

et al. 2013

CMC

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Fluorescence imaging techniques are becoming essential in preclinical investigations, and the research of suitable tools for in vivo measurements is gaining more and more importance and attention. Nanotechnology entered the field to try to find solutions for many limitation at the state of the art, and luminescent nanoparticles (NPs) are one of the most promising materials proposed for future diagnostic implementation. NPs constitute also a versatile platform that can allow facile multi-functionalization to perform multimodal imaging or theranostic (simultaneous diagnosis and therapy). In this contribution we have focussed our attention only on dye doped silica or silica-based NPs conjugated with targeting moieties to enable specific cancer cells imaging and differentiation, even if also a few non targeted systems have been cited and discussed for completeness. We have summarized common synthetic approaches to these materials and then surveyed the most recent imaging applications of silica-based nanoparticles in cancer. The field of theranostic is so important and stimulating that, even if it is not the central topic of this paper, we have included some significant examples. We have then concluded with short hints on systems already in clinical trials and examples of specific applications in children tumours. This review tries to describe and discuss, through focussed examples, the great potentialities of these materials in the medical field, with the aim to encourage further research to implement applications that are still rare.

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Targeted dual-color silica nanoparticles provide univocal identification of micrometastases in preclinical models of colorectal cancer

Cited by 17 publications

References 15 publications

Fabrication of Double Emission Enhancement Fluorescent Nanoparticles with Combined PET and AIEE Effects

Fabrication of Double Emission Enhancement Fluorescent Nanoparticles with Combined PET and AIEE Effects

Stem Cells and Nanomaterials

Luminescent Silica Nanoparticles for Cancer Diagnosis

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