Targeted Expression of miR-7 Operated by TTF-1 Promoter Inhibited the Growth of Human Lung Cancer through the NDUFA4 Pathway

Le, Lei; Chen, Chao; Zhao, Juanjuan; Wang, Hairong; Guo, Ming; Zhou, Yue; Luo, Jun; Zhang, Ji Dong; Xu, Lin

doi:10.1016/j.omtn.2016.12.005

Cited by 43 publications

(35 citation statements)

References 52 publications

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“…The present study showed that lncMIF‐AS1 can positively regulate the expression of NDUFA4. NDUFA4 encodes a protein belonging to the respiratory chain of mitochondria, which has been implicated in the development of various cancers including renal cell carcinoma and lung cancer . Herein, our study demonstrates that the expression of NDUFA4 is positively regulated by lncMIF‐AS1 in a micro RNA (miR)‐212‐5p‐targeting competitive endogenous way.…”

Section: Introductionmentioning

confidence: 80%

“…NDUFA4 gene is located on chromosome 7p21.3, which encodes a subunit of the electron transport chain complex belonging to the respiratory chain of mitochondria to produce ATP . Lei et al found that downregulation of NDUFA4 could contribute to the growth and metastasis of human lung cancer cells through altering the transduction of the Akt and Erk pathways. Downregulated NDUFA4 has also been detected in clear cell renal cell carcinoma, lung cancer, and esophageal squamous cell carcinoma .…”

Section: Discussionmentioning

confidence: 99%

“…NDUFA4 encodes a protein belonging to the respiratory chain of mitochondria, 13,14 which has been implicated in the development of various cancers including renal cell carcinoma and lung cancer. [15][16][17] Herein, our study demonstrates that the expression of NDUFA4 is positively regulated by lncMIF-AS1 in a micro RNA (miR)-212-5p-targeting competitive endogenous way. The overexpressed NDUFA4 further promotes the proliferation and inhibits the apoptosis of gastric cancer cells through activation of the oxidative phosphorylation pathway.…”

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confidence: 99%

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Long non‐coding RNA MIF‐AS1 promotes gastric cancer cell proliferation and reduces apoptosis to upregulate NDUFA4

Huang

et al. 2018

Cancer Science

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Long non‐coding RNA MIF‐AS1 (lncMIF‐AS1) has been found to be upregulated in the tumor tissues of gastric cancer; however, its importance for the progression of gastric cancer remains unknown. Thus, the present study was designed to determine the role of the lncMIF‐AS1‐based signal transduction pathway in mediating the proliferation and apoptosis of gastric cancer cells. Differentially expressed lncRNAs and mRNAs were screened out using microarray analysis, based on the published data (GSE63288), and validated using quantitative RT‐PCR. Target relationships between lncRNA‐micro RNA (miRNA) and miRNA‐mRNA were predicted by bioinformatics analysis and verified by dual‐luciferase reporter assay. Protein expression of NDUFA4, COX6C and COX5B was detected by western blot. Cell proliferation, cell cycle and apoptosis were determined using colony formation assay and flow cytometry analysis. Oxidative phosphorylation in gastric cancer cells was assessed by levels of oxygen consumption and ATP synthase activity. Expression of lncMIF‐AS1 and NDUFA4 were upregulated in gastric cancer tissues and cells as compared with non‐cancerous gastric tissues and cells (P < .05). MiR‐212‐5p was identified as the most important miRNA linker between lncMIF‐AS1 and NDUFA4, which was negatively regulated by lncMIF‐AS1 and its depletion is the main cause of NDUFA4 overexpression (P < .01). The upregulated expression of NDUFA4 then greatly promoted the proliferation and decreased the apoptosis of gastric cancer cells through activation of the oxidative phosphorylation pathway. Taken together, the present study implies that inhibition of lncMIF‐AS1/miR‐212‐5p/NDUFA4 signal transduction may provide a promising therapeutic target for the treatment of gastric cancer.

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Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Long non‐coding RNA MIF‐AS1 promotes gastric cancer cell proliferation and reduces apoptosis to upregulate NDUFA4

Huang

et al. 2018

Cancer Science

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“…MicroRNA-7 (miR-7) was first identified by Lagos-Quintana in 2001 [9] and has been reported to regulate the biology of various tumor cells and the development of inflammation diseases by repressing the expression of different target molecules [10,11]. For instance, Fan et al found that miR-7 suppressed angiogenesis of colorectal cancer cells through ERK signaling by downregulation epidermal growth factor receptor [12].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-7, synergizes with RORα, negatively controls the pathology of brain tissue inflammation

Yue

Zhao

Chen

et al. 2020

J Neuroinflammation

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Background: Accumulating evidence has documented that microRNA-7 (miR-7) plays an important role in the pathology of various diseases. However, the potential role of miR-7 in brain tissue inflammation (BTI) remains unclear. Methods: We detected the expression of miR-7 in LPS-induced murine BTI model and observed the possible effects of miR-7 deficiency on the pathology of BTI. To elucidate the mechanism, the target gene of miR-7 was screened out by Gene chip assay and its potential roles in BTI were evaluated by Western blot, immunofluorescence, and RNAi assay, respectively.Results: MiR-7 was upregulated in brain tissue in BTI mice and its deficiency could significantly aggravate the pathology of brain tissue. Moreover, RORα, a new target molecule of miR-7, was upregulated in brain tissue from miR-7 deficiency BTI mice. Of note, downregulation of RORα could remarkably exacerbate the pathology of brain tissue and elevate the transduction of NF-κB and ERK1/2 signaling pathways in brain tissue from miR-7 deficiency BTI mice. Furthermore, RORα and miR-7 were dominantly co-expressed in neurons of BTI mice. Finally, RORα synergized with miR-7 to control the inflammatory reaction of neuronal cells in response to LPS stimulation.Conclusions: MiR-7 expression is upregulated in BTI model. Moreover, miR-7 synergizes with its target gene RORα to control the inflammation reaction of neurons, thereby orchestrating the pathology of BTI.

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“…The RNA duplex is incorporated into the RNA-induced silencing complex, where one of the strands undergoes degradation while the other forms the mature miRNA [13]. Mature miRNAs can bind to the 3′-untranslated region of complementary or partially complementary target gene mRNA and promote the degradation of target gene mRNA at posttranscriptional level or mediate its translation inhibition, thus exerting a wide range of physiological regulatory functions [14]. In humans, more than 1,000 miRNA family members have been reported to participate in important biological processes such as cell development, differentiation, proliferation, apoptosis, tumorigenesis, and development.…”

Section: Introductionmentioning

confidence: 99%

Functional Role of MicroRNAs in Thymocyte Development

Mao

Liu

et al. 2019

Int Arch Allergy Immunol

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MicroRNAs (miRNAs) are a class of endogenous noncoding single-stranded RNAs widely distributed in eukaryotes, which can modulate target gene expression at posttranscriptional level and participate in cell proliferation, differentiation, and apoptosis. Related studies have shown that miRNAs are instrumental to many aspects of immunity, including various levels of T-cell immunity. In addition, multiple miRNAs have been ascribed key roles in T-cell development, differentiation, and function. In this review, we highlight the current literature regarding the functional role of miRNAs at various stages of thymocyte development. A better understanding of the relationship between miRNAs and thymocyte development is helpful for the exploration of the exact roles of miRNAs in the development and function of the immune system, as well as related clinical diseases.

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Targeted Expression of miR-7 Operated by TTF-1 Promoter Inhibited the Growth of Human Lung Cancer through the NDUFA4 Pathway

Cited by 43 publications

References 52 publications

Long non‐coding RNA MIF‐AS1 promotes gastric cancer cell proliferation and reduces apoptosis to upregulate NDUFA4

Long non‐coding RNA MIF‐AS1 promotes gastric cancer cell proliferation and reduces apoptosis to upregulate NDUFA4

MicroRNA-7, synergizes with RORα, negatively controls the pathology of brain tissue inflammation

Functional Role of MicroRNAs in Thymocyte Development

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