Targeted High-Resolution Taxonomic Identification of Bifidobacterium longum subsp. infantis Using Human Milk Oligosaccharide Metabolizing Genes

Tso, Lauren; Bonham, Kevin S.; Fishbein, Alyssa; Rowland, Sophie; Klepac‐Ceraj, Vanja

doi:10.3390/nu13082833

Cited by 11 publications

(9 citation statements)

References 54 publications

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“…Our results are consistent with studies reporting a higher prevalence of B. infantis , typically reaching more than 70%, in infants from countries with limited resources, such as Gambia (76.9%) and Bangladesh (86%), 7 or from rural settings in the USA. 10 The prevalence of this subspecies is lower (<20%) in more developed countries with higher rates of immune disorders, 11 , 12 , 49 as recently shown in a global meta-analysis. 13 We then studied the variation of the gut microbiome among the infants of our study cohort.…”

Section: Discussionmentioning

confidence: 79%

Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides

et al. 2023

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The gut microbiota evolves rapidly after birth, responding dynamically to environmental factors and playing a key role in short- and long-term health. Lifestyle and rurality have been shown to contribute to differences in the gut microbiome, including Bifidobacterium levels, between infants. We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants ( n = 105). Shotgun metagenomics showed Bifidobacterium longum to be the dominant species. A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp. infantis ( B. infantis ) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp. longum . Stratification of the gut microbiome into community (GMC) types revealed differences in composition and functional features. GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features. An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III ( Se+, Le -) (22%) than in most previously studied populations, with an enrichment in 2′-fucosyllactose. Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis , and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association. This study sheds light on gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors.

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Section: Discussionmentioning

confidence: 79%

Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides

et al. 2023

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“…longum were F: 5 ′ -TTCCAGTTGATCGCATGGTC-3 ′ and R: 5 ′ -GGGAAGCCGTATCTCTACGA-3 ′ . Primers and amplification methods were determined based on previous reports (22,23). For the determination of bacterial counts, bacterial solutions with already known their counts were used as a standard.…”

Section: Real-time Pcrmentioning

confidence: 99%

Dynamic Associations of Milk Components With the Infant Gut Microbiome and Fecal Metabolites in a Mother–Infant Model by Microbiome, NMR Metabolomic, and Time-Series Clustering Analyses

et al. 2022

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Background: The gut microbiome and fecal metabolites of breastfed infants changes during lactation, and are influenced by breast milk components. This study aimed to investigate dynamic associations of milk components with the infant gut microbiome and fecal metabolites throughout the lactation period in a mother–infant model.Methods: One month after delivery, breast milk and subsequent infant feces were collected in a pair for 5 months from a mother and an exclusively breastfed infant. Composition of the fecal microbiome was determined with 16S rRNA sequencing. Low-molecular-weight metabolites, including human milk oligosaccharides (HMOs), and antibacterial proteins were measured in feces and milk using 1H NMR metabolomics and enzyme-linked immunosorbent assays. The association of milk bioactive components with the infant gut microbiome and fecal metabolites was determined with Python clustering and correlation analyses.Results: The HMOs in milk did not fluctuate throughout the lactation period. However, they began to disappear in infant feces at the beginning of month 4. Notably, at this time-point, a bifidobacterium species switching (from B. breve to B. longum subsp. infantis) occurred, accompanied by fluctuations in several metabolites including acetate and butyrate in infant feces.Conclusions: Milk bioactive components, such as HMOs, might play different roles in the exclusively breastfed infants depending on the lactation period.

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“…Quantification of each Bifidobacteria species was performed using the following primers ( Table S1 ). Primers and amplification methods used were determined based on prior studies [ 39 , 40 , 41 ]. Bacterial copy numbers were determined by utilizing bacterial solutions with established counts as a standard.…”

Section: Methodsmentioning

confidence: 99%

Early Probiotic Supplementation of Healthy Term Infants with Bifidobacterium longum subsp. infantis M-63 Is Safe and Leads to the Development of Bifidobacterium-Predominant Gut Microbiota: A Double-Blind, Placebo-Controlled Trial

Hiraku¹,

Nakata

Murata³

et al. 2023

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Bifidobacteria are important intestinal bacteria that provide a variety of health benefits in infants. We investigated the efficacy and safety of Bifidobacterium longum subsp. infantis (B. infantis) M-63 in healthy infants in a double-blind, randomized, placebo-controlled trial. Healthy term infants were given B. infantis M-63 (n = 56; 1 × 109 CFU/day) or placebo (n = 54) from postnatal age ≤ 7 days to 3 months. Fecal samples were collected, and fecal microbiota, stool pH, short-chain fatty acids, and immune substances were analyzed. Supplementation with B. infantis M-63 significantly increased the relative abundance of Bifidobacterium compared with the placebo group, with a positive correlation with the frequency of breastfeeding. Supplementation with B. infantis M-63 led to decreased stool pH and increased levels of acetic acid and IgA in the stool at 1 month of age compared with the placebo group. There was a decreased frequency of defecation and watery stools in the probiotic group. No adverse events related to test foods were observed. These results indicate that early supplementation with B. infantis M-63 is well tolerated and contributes to the development of Bifidobacterium-predominant gut microbiota during a critical developmental phase in term infants.

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Targeted High-Resolution Taxonomic Identification of Bifidobacterium longum subsp. infantis Using Human Milk Oligosaccharide Metabolizing Genes

Cited by 11 publications

References 54 publications

Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides

Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides

Dynamic Associations of Milk Components With the Infant Gut Microbiome and Fecal Metabolites in a Mother–Infant Model by Microbiome, NMR Metabolomic, and Time-Series Clustering Analyses

Early Probiotic Supplementation of Healthy Term Infants with Bifidobacterium longum subsp. infantis M-63 Is Safe and Leads to the Development of Bifidobacterium-Predominant Gut Microbiota: A Double-Blind, Placebo-Controlled Trial

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