Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis

Zhang, Honglai; Yu, Ying; Zhang, Kejun; Liu, Xinfeng; Dai, Yan; Jiao, Xuelong

doi:10.1080/21655979.2019.1642722

Cited by 24 publications

(30 citation statements)

References 28 publications

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“… Cell line/tumor tissue Kim/2016 LOC100507661 3q26.2 C643 8505C – Up ↑ Cell proliferation, migration, and invasion a [ 11 ] Gu/2017 GAS5 1q25.1 – 33 Down ↑ Cell cycle progression and proliferation probably by upregulating of CDK6 ↓ Survival rate b Poor prognosis [ 12 ] Zhang/2019 H19 11p15.5 8505C SW1736 KAT18 CAL62 19 Up ↑ Proliferation, migration, and invasion ↓ Apoptosis [ 13 ] Wang/2018 UCA1 19p13.12 SW1736 KAT18 8 Up ↑ cell viability, proliferation, migration, and invasion via binding to miR-135a and regulation of c-myc expression [ 14 ] Wang/2019 HOTAIRM1 7p15.2 – 10 Up ↑ HOTAIRM1 expression during thyroid cancer development and progression [ 15 ] Wang/2019 PCA3 9q21.2 – 10 Up ↑ PCA3 expression during thyroid cancer development and progression [ 15 ] Chen/2019 HCP5 ...…”

Section: Resultsmentioning

confidence: 99%

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Molecular mechanisms of long non-coding RNAs in anaplastic thyroid cancer: a systematic review

et al. 2020

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Background anaplastic thyroid cancer (ATC) is one of the most lethal and aggressive cancers. Evidence has shown that the tumorigenesis of ATC is a multistep process involving the accumulation of genetic and epigenetic changes. Several studies have suggested that long non-coding RNAs (lncRNAs) may play an important role in the development and progression of ATC. In this article, we have collected the published reports about the role of lncRNAs in ATC. Methods “Scopus”, “Web of Science”, “PubMed”, “Embase”, etc. were systematically searched for articles published since 1990 to 2020 in English language, using the predefined keywords. Results 961 papers were reviewed and finally 33 papers which fulfilled the inclusion and exclusion criteria were selected. Based on this systematic review, among a lot of evidences on examining the function of lncRNAs in thyroid cancer, there are only a small number of studies about the role of lncRNAs and their molecular mechanisms in the pathogenesis of ATC. Conclusions lncRNAs play a crucial role in regulation of different processes involved in the development and progression of ATC. Currently, just a few lncRNAs have been identified in ATC that may serve as prognosis markers such as GAS5, MIR22HG, and CASC2. Also, because of the dysregulation of Klhl14-AS, HOTAIRM1, and PCA3 during ATC development and progression, they may act as therapeutic targets. However, for most lncRNAs, only a single experiment has evaluated the expression profile in ATC tissues/cells. Therefore, further functional studies and expression profiling is needed to resolve this limitation and identify novel and valid biomarkers.

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Section: Resultsmentioning

confidence: 99%

“…In this study, targeted inhibition of H19 with siRNA decreased proliferation, migration, and invasion and increased apoptosis in ATC cell line, 8505C, in vitro and inhibited tumorigenesis and metastasis in vivo. Therefore, the H19 may be an effective target for ATC molecular therapy [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanisms of long non-coding RNAs in anaplastic thyroid cancer: a systematic review

et al. 2020

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“…The dysregulation of lncRNA H19 has been studied widely in different cancers such as bladder (114), breast (115,116) and liver (117). The dysregulation of H19 in thyroid cancer is subject of controversy since the down-regulation was reported in FTC (118) and PTC (119), while the overexpression is reported in PTC, ATC (120) and PTC/PTC stem cells (PTCSCs) (121). Given the functional studies performed in these notes, the oncogenic nature of H19 in thyroid cancer is more conceivable since H19 knockdown resulted in the suppression of proliferation, migration, and invasion in ATC cells in vitro and inhibited tumorigenesis and metastasis in vivo (120).…”

Section: Lncrnas Regulating Thyroid Carcinogenesismentioning

confidence: 99%

“…The dysregulation of H19 in thyroid cancer is subject of controversy since the down-regulation was reported in FTC (118) and PTC (119), while the overexpression is reported in PTC, ATC (120) and PTC/PTC stem cells (PTCSCs) (121). Given the functional studies performed in these notes, the oncogenic nature of H19 in thyroid cancer is more conceivable since H19 knockdown resulted in the suppression of proliferation, migration, and invasion in ATC cells in vitro and inhibited tumorigenesis and metastasis in vivo (120). Moreover, the depletion of H19 was shown to suppress the sphere formation ability (121), collectively suggest that H19 acts as an oncogene in thyroid cancer.…”

Section: Lncrnas Regulating Thyroid Carcinogenesismentioning

confidence: 99%

Non-Coding RNAs: Uncharted Mediators of Thyroid Cancer Pathogenesis

Tabatabaeian¹,

Yang²,

Tay³

2020

Preprint

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Thyroid cancer is the most prevalent malignancy of the endocrine system and the ninth most common cancer globally. Despite the advances in the management of thyroid cancer, there are critical issues with the diagnosis and treatment of thyroid cancer that result in the poor overall survival of undifferentiated and metastatic thyroid cancer patients. Recent studies have revealed the role of different non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) that are dysregulated during thyroid cancer development or the acquisition of resistance to therapeutics, and may play key roles in treatment failure and poor prognosis of the thyroid cancer patients. Here, we systematically review the emerging roles and molecular mechanisms of ncRNAs that regulate thyroid tumorigenesis and drug response. We then propose the potential clinical implications of ncRNAs as novel diagnostic and prognostic biomarkers for thyroid cancer.

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“…9 Increasing studies demonstrated that H19 played a positive role in the embryonic development and tumorigenesis processes in multiple cancers. For example, zhang et al discovered that H19 expression was increased and H19 knockdown largely inhibited the progression of anaplastic thyroid carcinoma cells; 10 also, H19 significantly enhanced the malignant development of HCC cells by targeting miR-22. 11 Moreover, H19 has been identified to associate with the cell progression, cell chemosensitivity, poor prognosis and cell metastasis.…”

Section: Introductionmentioning

confidence: 99%

<p>Long Non-Coding RNA H19 Promotes Proliferation, Migration and Invasion and Inhibits Apoptosis of Breast Cancer Cells by Targeting miR-491-5p/ZNF703 Axis</p>

Wang

et al. 2020

CMAR

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Background: Breast cancer is one of the most common cancers worldwide. Long noncoding RNAs and microRNAs act as important regulators in human cancers. This study aims to explore the molecular mechanism among H19, miR-491-5p and zinc finger 703 (ZNF703) in breast cancer. Materials and Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to detect the expression of H19, miR-491-5p and ZNF703. Cell Counting Kit 8 (CCK-8) assay was performed to evaluate cell proliferation. Cell apoptosis was assessed by flow cytometry assay. The number of migrated and invaded cells was counted by transwell assay. Dual luciferase reporter assay was carried out to test luciferase activity. Protein level of ZNF703 was measured by Western blot assay. Results: H19 was highly expressed in breast tissues and cells. H19 knockdown inhibited proliferation, induced apoptosis and blocked migration and invasion. Moreover, H19 bound to miR-491-5p and negatively regulated miR-491-5p expression. MiR-491-5p inhibition abrogated the activities of proliferation, apoptosis, migration and invasion affected by H19 knockdown. Furthermore, miR-491-5p directly targeted ZNF703 and inversely modulated ZNF703 expression. ZNF703 up-regulation rescued the effects of miR-491-5p overexpression on proliferation, apoptosis, migration and invasion. In addition, H19 knockdown reduced ZNF703 expression by targeting miR-491-5p/ZNF703 axis. Conclusion: H19 promoted proliferation, migration and invasion and retarded apoptosis of breast cancer cells via sponging miR-491-5p to down-regulate ZNF703 expression.

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Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis

Cited by 24 publications

References 28 publications

Molecular mechanisms of long non-coding RNAs in anaplastic thyroid cancer: a systematic review

Molecular mechanisms of long non-coding RNAs in anaplastic thyroid cancer: a systematic review

Non-Coding RNAs: Uncharted Mediators of Thyroid Cancer Pathogenesis

<p>Long Non-Coding RNA H19 Promotes Proliferation, Migration and Invasion and Inhibits Apoptosis of Breast Cancer Cells by Targeting miR-491-5p/ZNF703 Axis</p>

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