Targeted Killing of Cancer Cells <i>in Vivo</i> and <i>in Vitro</i> with EGF-Directed Carbon Nanotube-Based Drug Delivery

Bhirde, Ashwin; Patel, Vyomesh; Gavard, Julie; Zhang, Guofeng; Sousa, Alioscka A.; Masedunskas, Andrius; Leapman, Richard D.; Weigert, Roberto; Gutkind, J. Silvio; Rusling, James F.

doi:10.1021/nn800551s

Cited by 813 publications

(542 citation statements)

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“…A particularly interesting is their use in the field of biomedicine: in biosensors [6,7], medical diagnostics [8,9], transplantology [10,11], tissue engineering [12] or in pharmacology -including the field of targeted therapies [13][14][15][16].…”

Section: Methods Of Dispersing Carbon Nanotubes -The Search For Optimmentioning

confidence: 99%

Congo Red Interactions with Single-Walled Carbon Nanotubes

Jagusiak

Piekarska

Chłopaś

et al. 2017

Self-Assembled Molecules – New Kind of Protein Ligands

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A new method of dispersion of single-wall carbon nanotubes (SWNT) in aqueous solution using supramolecular compounds is proposed in this chapter. The described system consists of SWNT overlaid by Congo red. SWNT are formed from a rolled layer of graphene, providing a large surface area for binding compounds with planar, aromatic structures (including drugs). Congo red is able to associate with proteins in the form of supramolecular, ribbon-like structures, and may bind various drugs by intercalation.The study reveals strong interactions between Congo red and the surface of SWNT. The authors' aim was to explain the mechanism driving this interaction. Spectral analysis of the SWNT-CR complex, effects of sonication on CR binding, microscopic imaging and molecular modelling analyses are all discussed. Results indicate that binding of supramolecular Congo red to the surface of nanotubes is based on face-to-face stacking. Having attached itself to the surface of a nanotube, a dye molecule may attract other similarly oriented molecules, giving rise to a protruding supramolecular appendage. This explains the high affinity of CR for nanotubes and the resulting system's capability to bind drugs.Analysis of complexes formed by SWNT-CR with the model drug (DOX) and with other planar compounds (EB, TY) indicates that it may be possible to construct complexes capable of binding multiple compounds simultaneously.

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Section: Methods Of Dispersing Carbon Nanotubes -The Search For Optimmentioning

confidence: 99%

Congo Red Interactions with Single-Walled Carbon Nanotubes

Jagusiak

Piekarska

Chłopaś

et al. 2017

Self-Assembled Molecules – New Kind of Protein Ligands

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“…poor solubility) and potential as the fullerene counterparts, such as drug design, drug delivery, tumor therapy, tissue engineering, DNA recognition, and biosensor design. [58][59][60][61][62] Besides, CNT is often used as a representative hydrophobic nanoparticle to study the role hydrophobic interaction plays in the protein-nanoparticle interaction, and the impact on the protein structure and function. Ruhong Zhou and co-workers took WW domains (i.e.…”

Section: Carbon Nanotube Cntmentioning

confidence: 99%

Computational studies on the interactions of nanomaterials with proteins and their impacts

An¹,

Su²,

Li³

et al. 2015

Chinese Phys. B

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Intensive concerns about the biosafety of nanomaterials demand the systematic study of the mechanisms about their biological effects. Many biological effects can be attributed to the interaction of nanomaterials with protein and their impacts on protein function. On the other hand, nanomaterials exhibit the potential in a variety of biomedical applications, many of which also involve the direct interaction with protein. In this paper, we review some recent computational studies about this subject, especially the interaction of carbon and gold nanomaterials. Besides the hydrophobic and π-stacking interactions, the interaction mode of carbon nanomaterials can be regulated by their functional groups. And the coating of gold nanomaterials also adjusts their interaction mode, in addition to the coordination interaction with cysteine's sulfur group and histidine's imidazole group. Moreover, nanomaterials can interact with multiple proteins and the impacts on protein activity are attributed to a wide spectrum of mechanisms. The findings about the mechanisms of nanomaterial-protein interaction can further guide the design and development of nanomaterial to realize the applications in disease diagnosis and treatment.

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“…Thus, cisplatin and EGF were attached to single-wall carbon nanotubes (SWNTs) to target squamous cancer cells HNSCC which overexpress EGFR. Through Qdot luminescence and confocal microscopy, it was shown that SWNT-Qdot-EGF bioconjugates was rapidly internalized into the cancer cells, and HNSCC cells were selectively killed in vitro, while tumor growth was regressed in vivo [31]. A current cancer treatment that targets EGFR is the monoclonal antibody Cetuximab, which targets the extracellular domain of EGFR and small-molecule inhibitors of tyrosine kinase activity [66].…”

Section: Human Epidermal Receptormentioning

confidence: 99%