Targeted metabolomics reveals reduced levels of polyunsaturated choline plasmalogens and a smaller dimethylarginine/arginine ratio in the follicular fluid of patients with a diminished ovarian reserve

Barca, Juan Manuel Chao de la; Boueilh, Thomas; Simard, Gilles; Boucret, Lisa; Ferré-L’Hôtellier, Véronique; Tessier, Lydie; Gadras, Cédric; Bouet, Pierre‐Emmanuel; Descamps, Philippe; Procaccio, Vincent; Reynier, Pascal; May‐Panloup, Pascale

doi:10.1093/humrep/dex303

Cited by 33 publications

(29 citation statements)

References 51 publications

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“…Changes in follicular microenvironment in older women may be responsible for altered protein role thus affecting the viability of oocytes. This certainly causes adverse pregnancy outcomes (13,14) .…”

Section: Discussionmentioning

confidence: 99%

Assessment Viability of Frozen-Thawed Oocytes Using Non- Invasive Technique based on Buffalo Follicular Fluid

M-R.Fakhrildin,

Abed Alkhazreji,

Mudhafar Abdulhussein

2021

Indian Journal of Forensic Medicine & Toxicology

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Back ground: Cryopreservation method, equilibrium rapid freezing (vitrification), used to preserve oocytes. Vitrification is a common method for cryopreservation of gametes and early embryos.The follicular fluid (FF) contains a complex mixture of steroids, metabolites, polysaccharides, proteins and small peptides, reactive oxygen species (ROS) and antioxidant enzymes (1) .Objectives: The present study was aimed to improve rate of buffalo oocyte viability using follicular fluid with Trypan blue, after thawing.Materials and Methods: Cryopreservation of oocytes using vitrification method using common solution, then thawing oocytes after 2 months and assessment oocyte viability. Collection of oocytes from buffalo ovaries buffalo after sacrifice, then assessment viability of oocytes using trypan blue stain for 30 seconds. Using vitrification solution (DMSO, EG), then the oocytes viable loaded on vitricare and plunged in liquid nitrogen (-196℃) for 2 months, after thawing, the oocytes viability was assessed, using two different concentrations of buffalo follicular fluid (low and high concentration) mixed with trypan blue, the SMART medium that used in the present research.Results: Results of the present study appeared significant increment (P<0.05) in the percentages of oocytes viability using follicular fluid with trypan blue as compared to the trypan blue only. Conclusion:From the results of the present study it was concluded that the follicular fluid enriched to the trypan blue improved percentage of oocytes viability post thawing.

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Section: Discussionmentioning

confidence: 99%

Assessment Viability of Frozen-Thawed Oocytes Using Non- Invasive Technique based on Buffalo Follicular Fluid

M-R.Fakhrildin,

Abed Alkhazreji,

Mudhafar Abdulhussein

2021

Indian Journal of Forensic Medicine & Toxicology

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“…Since FF constitutes a vital microenvironment for the development of oocytes, it seems logical to assume that some biochemical characteristics of FF surrounding the oocyte may imply ovarian reserve and may play a critical role in oocyte quality, fertilization potential, embryo development, and pregnancy rate. [18][19][20][21][22] Accordingly, we assessed whether FF activity of ADAT is different between infertile women with different infertility etiologies.…”

Section: Introductionmentioning

confidence: 99%

ADA activity in the follicular fluid of infertile women with diminished ovarian reserve can act as a predictor of ovarian reserve

İlhan¹,

Bacanakgıl²,

Köse³

et al. 2021

Int J Reprod Contracept Obstet Gynecol

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Background: Adenosine deaminase (ADA) catalyses the deamination of adenosine to inosine. In the human reproductive tract, the importance of enzymes that affect metabolism of adenosine, particularly ADA, has been emphasized. It is aimed to evaluate the plasma and follicular fluid (FF) activities of total ADA (ADAT) in infertile women and to determine its relation with ovarian reserve markers and in vitro fertilization (IVF) outcomes.Methods: Plasma and FF activities of ADAT were measured in 106 infertile women. Its relation with ovarian reserve markers and IVF outcomes were determined.Results: There was a significant difference in the ADAT activities between plasma and FF of infertile women (p<0.01). The activity of plasma ADAT was higher than FF ADAT in infertile women (p<0.01). The activity of FF ADAT in DOR group was higher than that of the others (p<0.01). In DOR group; the activity of FF ADAT activity had a negative correlation with BMI and a positive correlation with FSH and no relation with IVF outcomes.Conclusions: Increased ADAT activity can lead to reduced adenosine levels, which might be resulted in disturbed fertility process. The activity of FF ADAT activity might be important for fertility work-up. Further studies are needed.

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“…In some of the cases, the “unexplained” reason can be further explored later during in vitro fertilization treatments and reveal abnormalities such as low fertilization rate or abnormal eggs. However, in most cases, the “unexplained” etiology for infertility remains obscure [ 2 , 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Aging and the body mass index (BMI) are tightly associated with female fertility. Both factors have been suggested to influence the metabolic content of the FF and, in particular, the energy metabolism and lipid metabolism [ 4 , 5 , 6 , 7 , 8 , 9 ]. Surprisingly, while the mean number of mature oocytes was associated with fatty acid serum levels [ 10 ], BMI values did not associate with the fatty acid composition of the FF [ 11 ], leaving the mechanism for the association of maternal overweight to infertility thus far unknown [ 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

A Shift in Glycerolipid Metabolism Defines the Follicular Fluid of IVF Patients with Unexplained Infertility

et al. 2020

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Follicular fluid (FF) constitutes the microenvironment of the developing oocyte. We recently characterized its lipid composition and found lipid signatures of positive pregnancy outcome after in vitro fertilization (IVF). In the current study, we aimed to test the hypothesis that unexplained female infertility is related to lipid metabolism, given the lipid signature of positive-outcome IVF patients we previously found. Assuming that FF samples from IVF patients with male factor infertility can represent a non-hindered metabolic microenvironment, we compared them to FF taken from women with unexplained infertility. FF from patients undergoing IVF was examined for its lipid composition. We found highly increased triacylglycerol levels, with a lower abundance of monoacylglycerols, phospholipids and sphingolipids in the FF of patients with unexplained infertility. The alterations in the lipid class accumulation were independent of the body mass index (BMI) and were altogether kept across the age groups. Potential lipid biomarkers for pregnancy outcomes showed a highly discriminative abundance in the FF of unexplained infertility patients. Lipid abundance distinguished IVF patients with unrecognized infertility and provided a potential means for the evaluation of female fertility.

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Targeted metabolomics reveals reduced levels of polyunsaturated choline plasmalogens and a smaller dimethylarginine/arginine ratio in the follicular fluid of patients with a diminished ovarian reserve

Abstract: This study was supported by the University Hospital of Angers, the University of Angers, and the French national research centers, INSERM and the CNRS. There were no competing interests.

Cited by 33 publications

References 51 publications

Assessment Viability of Frozen-Thawed Oocytes Using Non- Invasive Technique based on Buffalo Follicular Fluid

Assessment Viability of Frozen-Thawed Oocytes Using Non- Invasive Technique based on Buffalo Follicular Fluid

ADA activity in the follicular fluid of infertile women with diminished ovarian reserve can act as a predictor of ovarian reserve

A Shift in Glycerolipid Metabolism Defines the Follicular Fluid of IVF Patients with Unexplained Infertility

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