Targeted mutagenesis of amino acid transporter genes for rice quality improvement using the CRISPR/Cas9 system

Wang, Shiyu; Yang, Ya-Chun; Guo, Ming; Zhong, Chongyuan; Yan, Chuan; Sun, Shengyuan

doi:10.1016/j.cj.2020.02.005

Cited by 67 publications

(32 citation statements)

References 34 publications

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“…Generally, all proteinaceous amino acids are transported through phloem from source leaves to seeds, and then are used for synthesis of proteins. Altering amino acid transport via regulating transporters can ultimately affect the amino acid and protein content of seeds [26,[97][98][99][100][101]. Rice is generally thought of as a cereal with lower grain protein content (GPC) [102], while significant genetic variations in GPC exist among different rice varieties [103].…”

Section: Seed/fruit Quality Improvementmentioning

confidence: 99%

Amino Acid Transporters in Plant Cells: A Brief Review

Yang

Wei

Huang

et al. 2020

Plants

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Amino acids are not only a nitrogen source that can be directly absorbed by plants, but also the major transport form of organic nitrogen in plants. A large number of amino acid transporters have been identified in different plant species. Despite belonging to different families, these amino acid transporters usually exhibit some general features, such as broad expression pattern and substrate selectivity. This review mainly focuses on transporters involved in amino acid uptake, phloem loading and unloading, xylem-phloem transfer, import into seed and intracellular transport in plants. We summarize the other physiological roles mediated by amino acid transporters, including development regulation, abiotic stress tolerance and defense response. Finally, we discuss the potential applications of amino acid transporters for crop genetic improvement.

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Section: Seed/fruit Quality Improvementmentioning

confidence: 99%

Amino Acid Transporters in Plant Cells: A Brief Review

Yang

Wei

Huang

et al. 2020

Plants

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“…By targeted mutagenesis of transporter genes of amino acids could bring significant variation among the mutants. The OsAAP6 and OsAAP10 are two mutants generated in rice high-yielding cultivars by Cas9, and these mutants showed significant improvement in the yield and cooking quality of rice [114]. From the above findings, we have reached a conclusion that further mutagenesis of amino acid genes could lead to the production of more high-yielding and good quality cultivars in rice.…”

Section: Use Of Crispr/cas9 For Improvement Of Yield and Qualitymentioning

confidence: 81%

A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises

Rasheed

Gill

Hassan

et al. 2021

CIMB

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Genome editing (GE) has revolutionized the biological sciences by creating a novel approach for manipulating the genomes of living organisms. Many tools have been developed in recent years to enable the editing of complex genomes. Therefore, a reliable and rapid approach for increasing yield and tolerance to various environmental stresses is necessary to sustain agricultural crop production for global food security. This critical review elaborates the GE tools used for crop improvement. These tools include mega-nucleases (MNs), such as zinc-finger nucleases (ZFNs), and transcriptional activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, this review addresses the latest advancements in the role of CRISPR/Cas9 for genome manipulation for major crop improvement, including yield and quality development of biotic stress- and abiotic stress-tolerant crops. Implementation of this technique will lead to the production of non-transgene crops with preferred characteristics that can result in enhanced yield capacity under various environmental stresses. The CRISPR/Cas9 technique can be combined with current and potential breeding methods (e.g., speed breeding and omics-assisted breeding) to enhance agricultural productivity to ensure food security. We have also discussed the challenges and limitations of CRISPR/Cas9. This information will be useful to plant breeders and researchers in the thorough investigation of the use of CRISPR/Cas9 to boost crops by targeting the gene of interest.

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“…Generally, the higher rice grain protein content (GPC) will lead to the worse ECQ; thus, the cultivars with good ECQ always are required to have relatively lower GPC, usually less than 7%. Wang et al (2020) used CRISPR/Cas9 system to knock out OsAAP6 and OsAAP10 in three high-yielding japonica varieties and one japonica line, respectively. The protein content of the mutants was decreased, and the ECQ was increased significantly.…”

Section: Proteinsmentioning

confidence: 99%

Crop Quality Improvement Through Genome Editing Strategy

Yang

Shen

et al. 2022

Front. Genome Ed.

Self Cite

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Good quality of crops has always been the most concerning aspect for breeders and consumers. However, crop quality is a complex trait affected by both the genetic systems and environmental factors, thus, it is difficult to improve through traditional breeding strategies. Recently, the CRISPR/Cas9 genome editing system, enabling efficiently targeted modification, has revolutionized the field of quality improvement in most crops. In this review, we briefly review the various genome editing ability of the CRISPR/Cas9 system, such as gene knockout, knock-in or replacement, base editing, prime editing, and gene expression regulation. In addition, we highlight the advances in crop quality improvement applying the CRISPR/Cas9 system in four main aspects: macronutrients, micronutrients, anti-nutritional factors and others. Finally, the potential challenges and future perspectives of genome editing in crop quality improvement is also discussed.

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Targeted mutagenesis of amino acid transporter genes for rice quality improvement using the CRISPR/Cas9 system

Cited by 67 publications

References 34 publications

Amino Acid Transporters in Plant Cells: A Brief Review

Amino Acid Transporters in Plant Cells: A Brief Review

A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises

Crop Quality Improvement Through Genome Editing Strategy

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