Targeted Small Interfering RNA-Immunoliposomes as a Promising Therapeutic Agent against Highly Pathogenic Avian Influenza A (H5N1) Virus Infection

Khantasup, Kannika; Kopermsub, Phikulthong; Chaichoun, Kridsada; Dharakul, Tararaj

doi:10.1128/aac.02768-13

Cited by 26 publications

(22 citation statements)

References 36 publications

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“…ILs were found to be more potent than nontargeted liposomes in reducing viral titer. These results indicate that the scFv plays a pivotal role in internalization of siRNA into virus-infected cells (33).…”

Section: Safe and Targeted Drug Delivery Using Scfv-viral Peptide Fusmentioning

confidence: 71%

“…Antibody-drug conjugates can be internalized from the cell surface through efficiently than nontargeted liposomes (33). They also compared the antiviral activity of both ILs and nontargeted liposomes on H5N1-infected Madine-Darby canine kidney cells to discover how much the scFv improves the siRNA delivery system.…”

Section: Safe and Targeted Drug Delivery Using Scfv-viral Peptide Fusmentioning

confidence: 99%

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Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery

Safdari

Ahmadzadeh

Khalili

et al. 2016

Mol Med

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Section: Safe and Targeted Drug Delivery Using Scfv-viral Peptide Fusmentioning

confidence: 71%

Section: Safe and Targeted Drug Delivery Using Scfv-viral Peptide Fusmentioning

confidence: 99%

Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery

Safdari

Ahmadzadeh

Khalili

et al. 2016

Mol Med

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“…The coding region close to the stop codon was often used as the target for the action of siRNA [9,12,15–16]. The other regions of the NP gene were also studied as targets [8,11–12 21].…”

Section: Resultsmentioning

confidence: 99%

High antiviral effect of TiO₂·PL–DNA nanocomposites targeted to conservative regions of (−)RNA and (+)RNA of influenza A virus in cell culture

Левина¹,

Репкова²,

Bessudnova³

et al. 2016

Beilstein J. Nanotechnol.

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Summary Background: The development of new antiviral drugs based on nucleic acids is under scrutiny. An important problem in this aspect is to find the most vulnerable conservative regions in the viral genome as targets for the action of these agents. Another challenge is the development of an efficient system for their delivery into cells. To solve this problem, we proposed a TiO2·PL–DNA nanocomposite consisting of titanium dioxide nanoparticles and polylysine (PL)-containing oligonucleotides. Results: The TiO2·PL–DNA nanocomposites bearing the DNA fragments targeted to different conservative regions of (−)RNA and (+)RNA of segment 5 of influenza A virus (IAV) were studied for their antiviral activity in MDCK cells infected with the H1N1, H5N1, and H3N2 virus subtypes. Within the negative strand of each of the studied strains, the efficiency of DNA fragments increased in the direction of its 3’-end. Thus, the DNA fragment aimed at the 3’-noncoding region of (−)RNA was the most efficient and inhibited the reproduction of different IAV subtypes by 3–4 orders of magnitude. Although to a lesser extent, the DNA fragments targeted at the AUG region of (+)RNA and the corresponding region of (−)RNA were also active. For all studied viral subtypes, the nanocomposites bearing the DNA fragments targeted to (−)RNA appeared to be more efficient than those containing fragments aimed at the corresponding (+)RNA regions. Conclusion: The proposed TiO2·PL–DNA nanocomposites can be successfully used for highly efficient and site-specific inhibition of influenza A virus of different subtypes. Some patterns of localization of the most vulnerable regions in IAV segment 5 for the action of DNA-based drugs were found. The (−)RNA strand of IAV segment 5 appeared to be more sensitive as compared to (+)RNA.

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“…The gene silencing effect was reportedly enhanced when the virus-infected MDCK cells were treated with the immunoliposomes 6-12 h post-infection. The study provided a beneficial concept which may further be utilized for development of delivery system for siRNAs in humans [63].…”

Section: • • Ribozymes and Dnazymesmentioning

confidence: 99%

Gene Silencing: A Therapeutic Approach to Combat Influenza Virus Infections

et al. 2015

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Selective gene silencing technologies such as RNA interference (RNAi) and nucleic acid enzymes have shown therapeutic potential for treating viral infections. Influenza virus is one of the major public health concerns around the world and its management is challenging due to a rapid increase in antiviral resistance. Influenza vaccine also has its limitations due to the emergence of new strains that may escape the immunity developed by the previous year's vaccine. Antiviral drugs are the primary mode of prevention and control against a pandemic and there is an urgency to develop novel antiviral strategies against influenza virus. In this review, we discuss the potential utility of several gene silencing mechanisms and their prophylactic and therapeutic potential against the influenza virus.

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Targeted Small Interfering RNA-Immunoliposomes as a Promising Therapeutic Agent against Highly Pathogenic Avian Influenza A (H5N1) Virus Infection

Cited by 26 publications

References 36 publications

Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery

Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery

High antiviral effect of TiO₂·PL–DNA nanocomposites targeted to conservative regions of (−)RNA and (+)RNA of influenza A virus in cell culture

Gene Silencing: A Therapeutic Approach to Combat Influenza Virus Infections

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Targeted Small Interfering RNA-Immunoliposomes as a Promising Therapeutic Agent against Highly Pathogenic Avian Influenza A (H5N1) Virus Infection

Cited by 26 publications

References 36 publications

Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery

Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery

High antiviral effect of TiO2·PL–DNA nanocomposites targeted to conservative regions of (−)RNA and (+)RNA of influenza A virus in cell culture

Gene Silencing: A Therapeutic Approach to Combat Influenza Virus Infections

Contact Info

Product

Resources

About

High antiviral effect of TiO₂·PL–DNA nanocomposites targeted to conservative regions of (−)RNA and (+)RNA of influenza A virus in cell culture