Targeted Transfer of Polyethylenimine–Avidin–DNA Bioconjugates to Hematopoietic Cells Using Biotinylated Monoclonal Antibodies

A Potential Targeting Gene Vector Based on Biotinylated Polyethyleneimine/Avidin Bioconjugates

“…Wojda et al (42,43) reported that targeting PEI-avidin-DNA to antibody biotinylated cells increased transfection efficiency several fold over untargeted PEI, and the surface membrane biotinylation could enhance the endocytosis of avidin bioconjugates into nucleated cells. In this paper, the transfection efficiency of ABP/pDNA complexes was compared with PEI/pDNA complexes in three different cell lines: HepG2 cells, 293 T cells and HeLa cells (shown in Fig.…”

Preparation and characterisation of antibody modified gelatin nanoparticles as drug carrier system for uptake in lymphocytes

“…Therefore, the binding efficiency of biotinylated monoclonal anti-CD3 antibody as targeting ligand for lymphocytic cells was evaluated. This antibody can specifically bind to the CD3 surface antigen of the T-cell receptor complex of T cells, leading to internalisation of the complex by a receptor mediated endocytotic pathway [24][25][26].…”

Polyethyleneimine‐based immunopolyplex for targeted gene transfer in human lymphoma celllines

“…The targeting of PEI‐polyplexes through an avidin–biotin antibody system was previously reported by Wodja et al 37. as a targeted transfer of PEI–avidin bioconjugates.…”

“…polylysine13, 28 or polyethyleneimine29–36. A more versatile targeting method is based on the attachment of biotinylated antibodies to specific proteins on the cell surface, with the subsequent addition of polyethyleneimine–avidin–DNA complexes37.…”