Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma

Abstract

The expression of annexin A2 (ANXA2) in nasopharyngeal carcinoma (NPC) cells induces the immunosuppressive response in dendritic cells; however, the oncogenic effect and clinical significance of ANXA2 have not been fully investigated in NPC cells. Immunohistochemical staining for ANXA2 was performed in 61 patients and the association with clinicopathological status was determined. Short hairpin (sh)RNA knockdown of ANXA2 was used to examine cellular effects of ANXA2, by investigating alterations in cell proliferation, migration, invasion, adhesion, tube-formation assay, and chemo-and radiosensitivity assays were performed. RT-qPCR, Western blotting, and immunofluorescence were applied to determine molecular expression levels. Clinical association studies showed that the expression of ANXA2 was significantly correlated with metastasis (p = 0.0326) and poor survival (p = 0.0256). Silencing of ANXA2 suppressed the abilities of cell proliferation, adhesion, migration, invasion, and vascular formation in NPC cell. ANXA2 up-regulated epithelial-mesenchymal transition associated signal proteins. Moreover, ANXA2 reduced sensitivities to irradiation and chemotherapeutic drugs. These results define ANXA2 as a novel prognostic factor for malignant processes, and it can serve as a molecular target of therapeutic interventions for NPC.

Annexin A2 is an independent prognostic biomarker for evaluating the malignant progression of laryngeal cancer

“…These results indicate that Annexin 2 may be an independent prognostic factor for hepatocellular carcinoma (20). Previous studies have demonstrated that the expression of Annexin A2 is significantly associated with metastasis and poor survival in nasopharyngeal and endometrial carcinoma, bladder cancer and serous ovarian cancer (21)(22)(23)(24). Using a proteomic assay, the present study identified that Annexin A2 was highly expressed in laryngeal carcinoma tissues, consistent with the results of the aforementioned studies.…”

“…The predictive efficiency of Annexin A2 combined with other Annexins in laryngeal carcinoma requires further investigation. Mechanically, silencing of Annexin A2 expression suppresses cell proliferation, adhesion, migration, invasion and vascular formation in nasopharyngeal carcinoma cells by downregulating epithelial-mesenchymal transition-associated signaling proteins (22). Decreased expression of Annexin A2 causes defects in tumor growth in vivo and cell proliferation in vitro without causing cytotoxicity.…”

Piperlongumine inhibits cancer stem cell properties and regulates multiple malignant phenotypes in oral cancer

“…The concentration, purity, and amount of total RNA were quantified using the Nano-Drop ND-1000 ultraviolet spectrophotometer (NanoDrop Technologies; Thermo Fisher Scientific, Inc.). Reverse transcription was conducted as previously described (31). qPCR was performed using the Fast SYBR ® -Green Master Mix (Invitrogen; Thermo Fisher Scientific, Inc.) on the ABI Step One Real-Time PCR system (ABI Corporation, Lee's Summit, MO, USA), according to the manufacturer's protocol.…”

Measurement based chip impedance determination on validation board

“…Such a method is expected to suffer from accuracy issue since the effect of IC mounting is not considered. Reference [2] precisely addresses the issue of erroneous chip impedance. Through measurement, antenna is redesigned iteratively to account for error in chip impedance [2].…”

“…Reference [2] precisely addresses the issue of erroneous chip impedance. Through measurement, antenna is redesigned iteratively to account for error in chip impedance [2]. However to reduce design cycle for inexpensive consumer wireless products it is preferable to have reasonably accurate chip impedance to start with.…”